ID   CRTQ_STAAN              Reviewed;         375 AA.
AC   Q7A3E0;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=4,4'-diaponeurosporenoate glycosyltransferase;
DE            EC=2.4.1.-;
GN   Name=crtQ; OrderedLocusNames=SA2350;
OS   Staphylococcus aureus (strain N315).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=N315;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=N315;
RA   Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.;
RT   "Shotgun proteomic analysis of total and membrane protein extracts of S.
RT   aureus strain N315.";
RL   Submitted (OCT-2007) to UniProtKB.
CC   -!- FUNCTION: Catalyzes the glycosylation of 4,4'-diaponeurosporenoate,
CC       i.e. the esterification of glucose at the C1'' position with the
CC       carboxyl group of 4,4'-diaponeurosporenic acid, to form glycosyl-4,4'-
CC       diaponeurosporenoate. This is a step in the biosynthesis of
CC       staphyloxanthin, an orange pigment present in most staphylococci
CC       strains (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Carotenoid biosynthesis; staphyloxanthin biosynthesis;
CC       staphyloxanthin from farnesyl diphosphate: step 4/5.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. CrtQ
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000018; BAB43654.1; -; Genomic_DNA.
DR   PIR; D90061; D90061.
DR   RefSeq; WP_000871744.1; NC_002745.2.
DR   AlphaFoldDB; Q7A3E0; -.
DR   SMR; Q7A3E0; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; BAB43654; BAB43654; BAB43654.
DR   KEGG; sau:SA2350; -.
DR   HOGENOM; CLU_038143_1_0_9; -.
DR   OMA; GWLGKPH; -.
DR   UniPathway; UPA00029; UER00559.
DR   Proteomes; UP000000751; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR001173; Glyco_trans_2-like.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF00535; Glycos_transf_2; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..375
FT                   /note="4,4'-diaponeurosporenoate glycosyltransferase"
FT                   /id="PRO_0000284864"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        277..297
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        330..350
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   375 AA;  42574 MW;  7EF96C86E9084DFD CRC64;
     MKWLSRILTV IVTMSMACGA LIFNRRHQLK TKTLNFNHKA LTIIIPARNE EKRIGHLLHS
     IIQQQVPVDV IVMNDGSTDE TARVARSYGA TVVDVVDDTD GKWYGKSHAC YQGVTHACTN
     RIAFVDADVT FLRKDAVETL INQYQLQGEK GLLSVQPYHI TKRFYEGFSA IFNLMTVVGM
     NVFSTLDDGR TNQHAFGPVT LTNKEDYYAT GGHKSANRHI IEGFALGSAY TSQSLPVTVY
     EGFPFVAFRM YQEGFQSLQE GWTKHLSTGA GGTKPKIMTA IVLWLFGSIA SILGLCLSLK
     YRQMSVRKMV ALYLSYTTQF IYLHRRVGQF SNLLMVCHPL LFMFFTKIFI QSWKQTHRYG
     VVEWKGRQYS ISKEQ