CRTSO_ARATH
ID CRTSO_ARATH Reviewed; 595 AA.
AC Q9M9Y8;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2008, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Prolycopene isomerase, chloroplastic;
DE Short=CrtISO;
DE EC=5.2.1.13;
DE AltName: Full=Carotenoid and chloroplast regulation protein 2;
DE AltName: Full=Carotenoid isomerase;
DE Flags: Precursor;
GN Name=CRTISO; Synonyms=CCR2; OrderedLocusNames=At1g06820; ORFNames=F4H5.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP FUNCTION, PATHWAY, AND ENZYME ACTIVITY.
RX PubMed=11884677; DOI=10.1105/tpc.010302;
RA Park H., Kreunen S.S., Cuttriss A.J., DellaPenna D., Pogson B.J.;
RT "Identification of the carotenoid isomerase provides insight into
RT carotenoid biosynthesis, prolamellar body formation, and
RT photomorphogenesis.";
RL Plant Cell 14:321-332(2002).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-57, CLEAVAGE OF TRANSIT PEPTIDE
RP [LARGE SCALE ANALYSIS] AFTER SER-56, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Carotene cis-trans-isomerase that converts 7,9,9'-tri-cis-
CC neurosporene to 9'-cis-neurosporene and 7,9,9',7'-tetra-cis-lycopene
CC (also known as prolycopene) into all-trans-lycopene. Isomerization
CC requires redox-active components, suggesting that isomerization is
CC achieved by a reversible redox reaction acting at specific double
CC bonds. Isomerizes adjacent cis-double bonds at C7 and C9 pairwise into
CC the trans-configuration, but is incapable of isomerizing single cis-
CC double bonds at C9 and C9'. Carotenoid biosynthesis is partly required
CC to form the prolamellar bodies of etioplasts.
CC {ECO:0000269|PubMed:11884677}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7,7',9,9'-tetra-cis-lycopene = all-trans-lycopene;
CC Xref=Rhea:RHEA:30971, ChEBI:CHEBI:15948, ChEBI:CHEBI:62466;
CC EC=5.2.1.13; Evidence={ECO:0000269|PubMed:11884677};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250};
CC Name=NADP(+); Xref=ChEBI:CHEBI:58349; Evidence={ECO:0000250};
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC {ECO:0000269|PubMed:11884677}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC CrtISO subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF63149.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC011001; AAF63149.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28042.1; -; Genomic_DNA.
DR EMBL; BX813870; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A86203; A86203.
DR RefSeq; NP_172167.2; NM_100559.4.
DR AlphaFoldDB; Q9M9Y8; -.
DR SMR; Q9M9Y8; -.
DR BioGRID; 22434; 1.
DR STRING; 3702.AT1G06820.1; -.
DR iPTMnet; Q9M9Y8; -.
DR PaxDb; Q9M9Y8; -.
DR PRIDE; Q9M9Y8; -.
DR ProteomicsDB; 220335; -.
DR EnsemblPlants; AT1G06820.1; AT1G06820.1; AT1G06820.
DR GeneID; 837193; -.
DR Gramene; AT1G06820.1; AT1G06820.1; AT1G06820.
DR KEGG; ath:AT1G06820; -.
DR Araport; AT1G06820; -.
DR TAIR; locus:2033055; AT1G06820.
DR eggNOG; KOG4254; Eukaryota.
DR HOGENOM; CLU_019722_4_1_1; -.
DR InParanoid; Q9M9Y8; -.
DR OMA; INYPKGG; -.
DR OrthoDB; 873686at2759; -.
DR PhylomeDB; Q9M9Y8; -.
DR BioCyc; ARA:AT1G06820-MON; -.
DR BioCyc; MetaCyc:AT1G06820-MON; -.
DR UniPathway; UPA00803; -.
DR PRO; PR:Q9M9Y8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9M9Y8; baseline and differential.
DR Genevisible; Q9M9Y8; AT.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046608; F:carotenoid isomerase activity; IMP:TAIR.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009662; P:etioplast organization; IMP:TAIR.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR014101; CrtISO.
DR InterPro; IPR045892; CrtISO-like.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR PANTHER; PTHR46313; PTHR46313; 1.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02730; carot_isom; 1.
PE 1: Evidence at protein level;
KW Acetylation; Carotenoid biosynthesis; Chloroplast; FAD; Flavoprotein;
KW Isomerase; Membrane; NAD; NADP; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..56
FT /note="Chloroplast"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 57..595
FT /note="Prolycopene isomerase, chloroplastic"
FT /id="PRO_0000225670"
FT MOD_RES 57
FT /note="N-acetylvaline"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 595 AA; 65428 MW; 01437095DF054001 CRC64;
MDLCFQNPVK CGDRLFSALN TSTYYKLGTS NLGFNGPVLE NRKKKKKLPR MVTVKSVSSS
VVASTVQGTK RDGGESLYDA IVIGSGIGGL VAATQLAVKE ARVLVLEKYL IPGGSSGFYE
RDGYTFDVGS SVMFGFSDKG NLNLITQALK AVGRKMEVIP DPTTVHFHLP NNLSVRIHRE
YDDFIAELTS KFPHEKEGIL GFYGDCWKIF NSLNSLELKS LEEPIYLFGQ FFQKPLECLT
LAYYLPQNAG AIARKYIKDP QLLSFIDAEC FIVSTVNALQ TPMINASMVL CDRHYGGINY
PVGGVGGIAK SLAEGLVDQG SEIQYKANVK SIILDHGKAV GVRLADGREF FAKTIISNAT
RWDTFGKLLK GEKLPKEEEN FQKVYVKAPS FLSIHMGVKA EVLPPDTDCH HFVLEDDWKN
LEEPYGSIFL SIPTILDSSL APDGRHILHI FTTSSIEDWE GLPPKEYEAK KEDVAARIIQ
RLEKKLFPGL SSSITFKEVG TPRTHRRFLA RDKGTYGPMP RGTPKGLLGM PFNTTAIDGL
YCVGDSCFPG QGVIAVAFSG VMCAHRVAAD IGLEKKSRVL DVGLLGLLGW LRTLA
