CRTSO_SOLLC
ID CRTSO_SOLLC Reviewed; 615 AA.
AC Q8S4R4;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Prolycopene isomerase, chloroplastic;
DE Short=CrtISO;
DE EC=5.2.1.13;
DE AltName: Full=Carotenoid isomerase;
DE AltName: Full=Protein tangerine;
DE Flags: Precursor;
GN Name=CRTISO;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, ENZYME ACTIVITY, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=11884678; DOI=10.1105/tpc.010303;
RA Isaacson T., Ronen G., Zamir D., Hirschberg J.;
RT "Cloning of tangerine from tomato reveals a carotenoid isomerase essential
RT for the production of beta-carotene and xanthophylls in plants.";
RL Plant Cell 14:333-342(2002).
RN [2]
RP ENZYME ACTIVITY, COFACTOR, AND SUBCELLULAR LOCATION.
RX PubMed=15557094; DOI=10.1104/pp.104.052092;
RA Isaacson T., Ohad I., Beyer P., Hirschberg J.;
RT "Analysis in vitro of the enzyme CRTISO establishes a poly-cis-carotenoid
RT biosynthesis pathway in plants.";
RL Plant Physiol. 136:4246-4255(2004).
CC -!- FUNCTION: Carotene cis-trans-isomerase that converts 7,9,9'-tri-cis-
CC neurosporene to 9'-cis-neurosporene and 7,9,9',7'-tetra-cis-lycopene
CC (also known as prolycopene) into all-trans-lycopene. Isomerization
CC requires redox-active components, suggesting that isomerization is
CC achieved by a reversible redox reaction acting at specific double
CC bonds. Isomerizes adjacent cis-double bonds at C7 and C9 pairwise into
CC the trans-configuration, but is incapable of isomerizing single cis-
CC double bonds at C9 and C9'.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7,7',9,9'-tetra-cis-lycopene = all-trans-lycopene;
CC Xref=Rhea:RHEA:30971, ChEBI:CHEBI:15948, ChEBI:CHEBI:62466;
CC EC=5.2.1.13; Evidence={ECO:0000269|PubMed:11884678,
CC ECO:0000269|PubMed:15557094};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000269|PubMed:15557094};
CC Name=NADP(+); Xref=ChEBI:CHEBI:58349;
CC Evidence={ECO:0000269|PubMed:15557094};
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000269|PubMed:15557094};
CC -!- PATHWAY: Carotenoid biosynthesis; lycopene biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000305|PubMed:15557094}; Peripheral membrane protein
CC {ECO:0000305|PubMed:15557094}.
CC -!- TISSUE SPECIFICITY: Expressed in fruits, with a strong expression
CC during the breaker stage of fruit ripening.
CC {ECO:0000269|PubMed:11884678}.
CC -!- DISRUPTION PHENOTYPE: Plants display fruits with orange color due to an
CC accumulation of prolycopene, explaining the tangerine mutant name.
CC {ECO:0000269|PubMed:11884678}.
CC -!- SIMILARITY: Belongs to the carotenoid/retinoid oxidoreductase family.
CC CrtISO subfamily. {ECO:0000305}.
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DR EMBL; AF416727; AAL91366.1; -; Genomic_DNA.
DR RefSeq; NP_001296159.1; NM_001309230.1.
DR RefSeq; NP_001296160.1; NM_001309231.1.
DR RefSeq; NP_001296161.1; NM_001309232.1.
DR AlphaFoldDB; Q8S4R4; -.
DR SMR; Q8S4R4; -.
DR STRING; 4081.Solyc10g081650.1.1; -.
DR PaxDb; Q8S4R4; -.
DR PRIDE; Q8S4R4; -.
DR EnsemblPlants; Solyc10g081650.2.1; Solyc10g081650.2.1; Solyc10g081650.2.
DR GeneID; 101267857; -.
DR Gramene; Solyc10g081650.2.1; Solyc10g081650.2.1; Solyc10g081650.2.
DR KEGG; sly:101267857; -.
DR eggNOG; KOG4254; Eukaryota.
DR HOGENOM; CLU_019722_4_1_1; -.
DR InParanoid; Q8S4R4; -.
DR OMA; INYPKGG; -.
DR OrthoDB; 873686at2759; -.
DR PhylomeDB; Q8S4R4; -.
DR BioCyc; MetaCyc:MON-12167; -.
DR UniPathway; UPA00803; -.
DR Proteomes; UP000004994; Chromosome 10.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046608; F:carotenoid isomerase activity; IEA:EnsemblPlants.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009662; P:etioplast organization; IEA:EnsemblPlants.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002937; Amino_oxidase.
DR InterPro; IPR014101; CrtISO.
DR InterPro; IPR045892; CrtISO-like.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR PANTHER; PTHR46313; PTHR46313; 1.
DR Pfam; PF01593; Amino_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR02730; carot_isom; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Chloroplast; FAD; Flavoprotein; Isomerase;
KW Membrane; NAD; NADP; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..615
FT /note="Prolycopene isomerase, chloroplastic"
FT /id="PRO_0000225672"
SQ SEQUENCE 615 AA; 67455 MW; 92734B57F6005D89 CRC64;
MCTLSFMYPN SLLDGTCKTV ALGDSKPRYN KQRSSCFDPL IIGNCTDQQQ LCGLSWGVDK
AKGRRGGTVS NLKAVVDVDK RVESYGSSDV EGNESGSYDA IVIGSGIGGL VAATQLAVKG
AKVLVLEKYV IPGGSSGFYE RDGYKFDVGS SVMFGFSDKG NLNLITQALA AVGRKLEVIP
DPTTVHFHLP NDLSVRIHRE YDDFIEELVS KFPHEKEGII KFYSECWKIF NSLNSLELKS
LEEPIYLFGQ FFKKPLECLT LAYYLPQNAG SIARKYIRDP GLLSFIDAEC FIVSTVNALQ
TPMINASMVL CDRHFGGINY PVGGVGEIAK SLAKGLDDHG SQILYRANVT SIILDNGKAV
GVKLSDGRKF YAKTIVSNAT RWDTFGKLLK AENLPKEEEN FQKAYVKAPS FLSIHMGVKA
DVLPPDTDCH HFVLEDDWTN LEKPYGSIFL SIPTVLDSSL APEGHHILHI FTTSSIEDWE
GLSPKDYEAK KEVVAERIIS RLEKTLFPGL KSSILFKEVG TPKTHRRYLA RDSGTYGPMP
RGTPKGLLGM PFNTTAIDGL YCVGDSCFPG QGVIAVAFSG VMCAHRVAAD LGFEKKSDVL
DSALLRLLGW LRTLA
