ID   CRTW_PARSN              Reviewed;         242 AA.
AC   P54972; Q33DU0;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=Beta-carotene ketolase;
DE            EC=1.14.99.63;
DE   AltName: Full=Beta-carotene oxygenase;
GN   Name=crtW;
OS   Paracoccus sp. (strain N81106 / MBIC 01143) (Agrobacterium aurantiacum).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus; unclassified Paracoccus (in: Bacteria).
OX   NCBI_TaxID=81397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7592436; DOI=10.1128/jb.177.22.6575-6584.1995;
RA   Misawa N., Satomi Y., Kondo K., Yokoyama A., Kajiwara S., Saito T.,
RA   Ohtani T., Miki W.;
RT   "Structure and functional analysis of a marine bacterial carotenoid
RT   biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at
RT   the gene level.";
RL   J. Bacteriol. 177:6575-6584(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Maruyama T., Inomata Y., Haga M., Ide T., Misawa N.;
RT   "Structure of the complete carotenoid biosynthesis gene cluster of
RT   Paracoccus sp. strain N81106.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Converts beta-carotene to canthaxanthin via echinenone.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 AH2 + all-trans-beta-carotene + 2 O2 = 2 A + echinenone + 3
CC         H2O; Xref=Rhea:RHEA:55660, ChEBI:CHEBI:4746, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:17579; EC=1.14.99.63;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 AH2 + echinenone + 2 O2 = 2 A + canthaxanthin + 3 H2O;
CC         Xref=Rhea:RHEA:55664, ChEBI:CHEBI:3362, ChEBI:CHEBI:4746,
CC         ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17499; EC=1.14.99.63;
CC   -!- PATHWAY: Carotenoid biosynthesis; astaxanthin biosynthesis.
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DR   EMBL; D58420; BAA09591.1; -; Genomic_DNA.
DR   EMBL; AB206672; BAE47465.1; -; Genomic_DNA.
DR   AlphaFoldDB; P54972; -.
DR   BRENDA; 1.14.11.B16; 12605.
DR   BRENDA; 1.14.99.63; 15358.
DR   UniPathway; UPA00387; -.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR005804; FA_desaturase_dom.
DR   InterPro; IPR012171; Fatty_acid_desaturase.
DR   PANTHER; PTHR19353; PTHR19353; 1.
DR   Pfam; PF00487; FA_desaturase; 2.
PE   4: Predicted;
KW   Carotenoid biosynthesis; Oxidoreductase.
FT   CHAIN           1..242
FT                   /note="Beta-carotene ketolase"
FT                   /id="PRO_0000079370"
SQ   SEQUENCE   242 AA;  27128 MW;  6AEBB5BF60BE3D6B CRC64;
     MSAHALPKAD LTATSLIVSG GIIAAWLALH VHALWFLDAA AHPILAIANF LGLTWLSVGL
     FIIAHDAMHG SVVPGRPRAN AAMGQLVLWL YAGFSWRKMI VKHMAHHRHA GTDDDPDFDH
     GGPVRWYARF IGTYFGWREG LLLPVIVTVY ALILGDRWMY VVFWPLPSIL ASIQLFVFGT
     WLPHRPGHDA FPDRHNARSS RISDPVSLLT CFHFGGYHHE HHLHPTVPWW RLPSTRTKGD
     TA