ID   CRTX_PANAN              Reviewed;         431 AA.
AC   P21686;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Zeaxanthin glucosyltransferase;
DE            EC=2.4.1.276;
GN   Name=crtX; Synonyms=ugt101;
OS   Pantoea ananas (Erwinia uredovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=553;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19321 / DSM 30080 / NCPPB 800 / NRRL B-14773 / 20D3;
RX   PubMed=2254247; DOI=10.1128/jb.172.12.6704-6712.1990;
RA   Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K.,
RA   Harashima K.;
RT   "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by
RT   functional analysis of gene products expressed in Escherichia coli.";
RL   J. Bacteriol. 172:6704-6712(1990).
CC   -!- FUNCTION: Catalyzes the glycosylation reaction which converts
CC       zeaxanthin to zeaxanthin bis(beta-D-glucoside). The reaction proceeds
CC       in two steps with the monoglucoside as an intermediate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-zeaxanthin + 2 UDP-alpha-D-glucose = 2 H(+) + 2 UDP
CC         + zeaxanthin bis(beta-D-glucoside); Xref=Rhea:RHEA:31543,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:27547, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885, ChEBI:CHEBI:63067; EC=2.4.1.276;
CC   -!- PATHWAY: Carotenoid biosynthesis; zeaxanthin diglucoside biosynthesis.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; D90087; BAA14125.1; -; Genomic_DNA.
DR   PIR; B37802; B37802.
DR   AlphaFoldDB; P21686; -.
DR   SMR; P21686; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   BRENDA; 2.4.1.276; 2137.
DR   UniPathway; UPA00798; -.
DR   GO; GO:0016758; F:hexosyltransferase activity; IEA:InterPro.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   InterPro; IPR006326; UDPGT_MGT-like.
DR   Pfam; PF00201; UDPGT; 1.
DR   TIGRFAMs; TIGR01426; MGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..431
FT                   /note="Zeaxanthin glucosyltransferase"
FT                   /id="PRO_0000074163"
SQ   SEQUENCE   431 AA;  47241 MW;  9ECE7A798608BC9D CRC64;
     MSHFAAIAPP FYSHVRALQN LAQELVARGH RVTFIQQYDI KHLIDSETIG FHSVGTDSHP
     PGALTRVLHL AAHPLGPSML KLINEMARTT DMLCRELPQA FNDLAVDGVI VDQMEPAGAL
     VAEALGLPFI SVACALPLNR EPDMPLAVMP FEYGTSDAAR ERYAASEKIY DWLMRRHDRV
     IAEHSHRMGL APRQKLHQCF SPLAQISQLV PELDFPRKAL PACFHAVGPL RETHAPSTSS
     SRYFTSSEKP RIFASLGTLQ GHRYGLFKTI VKACEEIDGQ LLLAHCGRLT DSQCEELARS
     RHTQVVDFAD QSAALSQAQL AITHGGMNTV LDAINYRTPL LALPLAFDQP GVASRIVYHG
     IGKRASRFTT SHALARQMRS LLTNVDFQQR MAKIQTALRL AGGTMAAADI IEQVMCTGQP
     VLSGSGYATA L