ID   CRTX_PSEVU              Reviewed;         413 AA.
AC   Q01330;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Zeaxanthin glucosyltransferase;
DE            EC=2.4.1.276;
GN   Name=crtX; Synonyms=ugt101;
OS   Pseudescherichia vulneris (Escherichia vulneris).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Pseudescherichia.
OX   NCBI_TaxID=566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 39368 / Eho10;
RX   PubMed=1409639; DOI=10.1073/pnas.89.19.9321;
RA   Hundle B.S., O'Brien D.A., Alberti M., Beyer P., Hearst J.E.;
RT   "Functional expression of zeaxanthin glucosyltransferase from Erwinia
RT   herbicola and a proposed uridine diphosphate binding site.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:9321-9325(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 39368 / Eho10;
RX   PubMed=7808389; DOI=10.1007/bf00302252;
RA   Hundle B., Alberti M., Nievelstein V., Beyer P., Kleinig H.,
RA   Armstrong G.A., Burke D.H., Hearst J.E.;
RT   "Functional assignment of Erwinia herbicola Eho10 carotenoid genes
RT   expressed in Escherichia coli.";
RL   Mol. Gen. Genet. 245:406-416(1994).
CC   -!- FUNCTION: Catalyzes the glycosylation reaction which converts
CC       zeaxanthin to zeaxanthin bis(beta-D-glucoside). The reaction proceeds
CC       in two steps with the monoglucoside as an intermediate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-zeaxanthin + 2 UDP-alpha-D-glucose = 2 H(+) + 2 UDP
CC         + zeaxanthin bis(beta-D-glucoside); Xref=Rhea:RHEA:31543,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:27547, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885, ChEBI:CHEBI:63067; EC=2.4.1.276;
CC         Evidence={ECO:0000269|PubMed:1409639};
CC   -!- PATHWAY: Carotenoid biosynthesis; zeaxanthin diglucoside biosynthesis.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M87280; AAA64979.1; -; Genomic_DNA.
DR   PIR; S52980; S52980.
DR   AlphaFoldDB; Q01330; -.
DR   SMR; Q01330; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   KEGG; ag:AAA64979; -.
DR   BRENDA; 2.4.1.276; 12671.
DR   UniPathway; UPA00798; -.
DR   GO; GO:0046527; F:glucosyltransferase activity; IDA:CACAO.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR007235; Glyco_trans_28_C.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   InterPro; IPR006326; UDPGT_MGT-like.
DR   Pfam; PF04101; Glyco_tran_28_C; 1.
DR   Pfam; PF00201; UDPGT; 1.
DR   TIGRFAMs; TIGR01426; MGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..413
FT                   /note="Zeaxanthin glucosyltransferase"
FT                   /id="PRO_0000074162"
SQ   SEQUENCE   413 AA;  44853 MW;  353406A12B27D2E1 CRC64;
     MSHFAIVAPP LYSHAVALHA LALEMAQRGH RVTFLTGNVA SLAEQETERV AFYPLPASVQ
     QAQRNVQQQS NGNLLRLIAA MSSLTDVLCQ QLPAILQRLA VDALIVDEME PAGSLVAEAL
     GLPFISIACA LPVNREPGLP LPVMPFHYAE DKRALRRFQV SERIYDALMY PHGQTILRHA
     QRFGLPERRR LDECLSPLAQ ISQSVPALDF PRRALPNCFH YVGALRYQPP PQVERSPRST
     PRIFASLGTL QGHRLRLFQK IARACASVGA EVTIAHCDGL TPAQADSLYA CGATEVVSFV
     DQPRYVAEAN LVITHGGLNT VLDALAAATP VLAVPLSFDQ PAVAARLVYN GLGRRVSRFA
     RQQTLADEIA QLLGDETLHQ RLATARQQLN DAGGTPRAAT LIEQAIAGSE SVS