ID   CRTZ_PANAN              Reviewed;         175 AA.
AC   P21688;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Beta-carotene hydroxylase;
DE            EC=1.14.15.24 {ECO:0000250|UniProtKB:Q9SZZ8};
GN   Name=crtZ;
OS   Pantoea ananas (Erwinia uredovora).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=553;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19321 / DSM 30080 / NCPPB 800 / NRRL B-14773 / 20D3;
RX   PubMed=2254247; DOI=10.1128/jb.172.12.6704-6712.1990;
RA   Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K.,
RA   Harashima K.;
RT   "Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by
RT   functional analysis of gene products expressed in Escherichia coli.";
RL   J. Bacteriol. 172:6704-6712(1990).
CC   -!- FUNCTION: Catalyzes the hydroxylation reaction from beta-carotene to
CC       zeaxanthin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-beta-carotene + 4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-
CC         [ferredoxin] = all-trans-zeaxanthin + 2 H2O + 4 oxidized [2Fe-2S]-
CC         [ferredoxin]; Xref=Rhea:RHEA:30331, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17579, ChEBI:CHEBI:27547, ChEBI:CHEBI:33737,
CC         ChEBI:CHEBI:33738; EC=1.14.15.24;
CC         Evidence={ECO:0000250|UniProtKB:Q9SZZ8};
CC   -!- PATHWAY: Carotenoid biosynthesis; zeaxanthin biosynthesis.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
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DR   EMBL; D90087; BAA14129.1; -; Genomic_DNA.
DR   PIR; F37802; F37802.
DR   AlphaFoldDB; P21688; -.
DR   BioCyc; MetaCyc:MON-14937; -.
DR   BRENDA; 1.14.15.24; 2137.
DR   UniPathway; UPA00843; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR045019; BETA-OHASE-like.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   PANTHER; PTHR31899; PTHR31899; 1.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Oxidoreductase.
FT   CHAIN           1..175
FT                   /note="Beta-carotene hydroxylase"
FT                   /id="PRO_0000079379"
FT   DOMAIN          11..136
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   175 AA;  19816 MW;  4EE1E57011C51F9A CRC64;
     MLWIWNALIV FVTVIGMEVI AALAHKYIMH GWGWGWHLSH HEPRKGAFEV NDLYAVVFAA
     LSILLIYLGS TGMWPLQWIG AGMTAYGLLY FMVHDGLVHQ RWPFRYIPRK GYLKRLYMAH
     RMHHAVRGKE GCVSFGFLYA PPLSKLQATL RERHGARAGA ARDAQGGEDE PASGK