ID   CRTZ_PARS1              Reviewed;         162 AA.
AC   Q44262;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=Beta-carotene hydroxylase;
DE            EC=1.14.15.24 {ECO:0000250|UniProtKB:Q9SZZ8};
GN   Name=crtZ;
OS   Paracoccus sp. (strain PC1) (Alcaligenes sp. (strain PC1)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus; unclassified Paracoccus (in: Bacteria).
OX   NCBI_TaxID=365615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7733978; DOI=10.1006/bbrc.1995.1579;
RA   Misawa N., Kajiwara S., Kondo K., Yokoyama A., Satomi Y., Saito T.,
RA   Miki W., Ohtani T.;
RT   "Canthaxanthin biosynthesis by the conversion of methylene to keto groups
RT   in a hydrocarbon beta-carotene by a single gene.";
RL   Biochem. Biophys. Res. Commun. 209:867-876(1995).
CC   -!- FUNCTION: Catalyzes the hydroxylation reaction from beta-carotene to
CC       zeaxanthin via beta-cryptoxanthin. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-beta-carotene + 4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-
CC         [ferredoxin] = all-trans-zeaxanthin + 2 H2O + 4 oxidized [2Fe-2S]-
CC         [ferredoxin]; Xref=Rhea:RHEA:30331, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17579, ChEBI:CHEBI:27547, ChEBI:CHEBI:33737,
CC         ChEBI:CHEBI:33738; EC=1.14.15.24;
CC         Evidence={ECO:0000250|UniProtKB:Q9SZZ8};
CC   -!- PATHWAY: Carotenoid biosynthesis; astaxanthin biosynthesis.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
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DR   EMBL; D58422; BAA09597.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q44262; -.
DR   BRENDA; 1.14.15.24; 15481.
DR   UniPathway; UPA00387; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR045019; BETA-OHASE-like.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   PANTHER; PTHR31899; PTHR31899; 1.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Oxidoreductase.
FT   CHAIN           1..162
FT                   /note="Beta-carotene hydroxylase"
FT                   /id="PRO_0000079377"
FT   DOMAIN          8..135
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   162 AA;  19157 MW;  0CA5235CBFEB33B5 CRC64;
     MTQFLIVVAT VLVMELTAYS VHRWIMHGPL GWGWHKSHHE EHDHALEKND LYGVVFAVLA
     TILFTVGAYW WPVLWWIALG MTVYGLIYFI LHDGLVHQRW PFRYIPRRGY FRRLYQAHRL
     HHAVEGRDHC VSFGFIYAPP VDKLKQDLKR SGVLRPQDER PS