ID   CRTZ_PARSN              Reviewed;         162 AA.
AC   P54973; Q33DT9;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Beta-carotene hydroxylase;
DE            EC=1.14.15.24 {ECO:0000250|UniProtKB:Q9SZZ8};
GN   Name=crtZ;
OS   Paracoccus sp. (strain N81106 / MBIC 01143) (Agrobacterium aurantiacum).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Paracoccus; unclassified Paracoccus (in: Bacteria).
OX   NCBI_TaxID=81397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7592436; DOI=10.1128/jb.177.22.6575-6584.1995;
RA   Misawa N., Satomi Y., Kondo K., Yokoyama A., Kajiwara S., Saito T.,
RA   Ohtani T., Miki W.;
RT   "Structure and functional analysis of a marine bacterial carotenoid
RT   biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at
RT   the gene level.";
RL   J. Bacteriol. 177:6575-6584(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Maruyama T., Inomata Y., Haga M., Ide T., Misawa N.;
RT   "Structure of the complete carotenoid biosynthesis gene cluster of
RT   Paracoccus sp. strain N81106.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the hydroxylation reaction from beta-carotene to
CC       zeaxanthin via beta-cryptoxanthin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-beta-carotene + 4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-
CC         [ferredoxin] = all-trans-zeaxanthin + 2 H2O + 4 oxidized [2Fe-2S]-
CC         [ferredoxin]; Xref=Rhea:RHEA:30331, Rhea:RHEA-COMP:10000, Rhea:RHEA-
CC         COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17579, ChEBI:CHEBI:27547, ChEBI:CHEBI:33737,
CC         ChEBI:CHEBI:33738; EC=1.14.15.24;
CC         Evidence={ECO:0000250|UniProtKB:Q9SZZ8};
CC   -!- PATHWAY: Carotenoid biosynthesis; astaxanthin biosynthesis.
CC   -!- SIMILARITY: Belongs to the sterol desaturase family. {ECO:0000305}.
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DR   EMBL; D58420; BAA09592.1; -; Genomic_DNA.
DR   EMBL; AB206672; BAE47466.1; -; Genomic_DNA.
DR   AlphaFoldDB; P54973; -.
DR   BRENDA; 1.14.15.24; 4532.
DR   UniPathway; UPA00387; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR045019; BETA-OHASE-like.
DR   InterPro; IPR006694; Fatty_acid_hydroxylase.
DR   PANTHER; PTHR31899; PTHR31899; 1.
DR   Pfam; PF04116; FA_hydroxylase; 1.
PE   3: Inferred from homology;
KW   Carotenoid biosynthesis; Oxidoreductase.
FT   CHAIN           1..162
FT                   /note="Beta-carotene hydroxylase"
FT                   /id="PRO_0000079376"
FT   DOMAIN          8..135
FT                   /note="Fatty acid hydroxylase"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   162 AA;  18926 MW;  FB8891AA12A92C6C CRC64;
     MTNFLIVVAT VLVMELTAYS VHRWIMHGPL GWGWHKSHHE EHDHALEKND LYGLVFAVIA
     TVLFTVGWIW APVLWWIALG MTVYGLIYFV LHDGLVHQRW PFRYIPRKGY ARRLYQAHRL
     HHAVEGRDHC VSFGFIYAPP VDKLKQDLKM SGVLRAEAQE RT