ID   CRT_CLODI               Reviewed;         155 AA.
AC   P45361;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Short-chain-enoyl-CoA hydratase {ECO:0000250|UniProtKB:P52046};
DE            EC=4.2.1.150 {ECO:0000250|UniProtKB:P52046};
DE   AltName: Full=3-hydroxybutyryl-CoA dehydratase {ECO:0000250|UniProtKB:P52046};
DE   AltName: Full=Crotonase {ECO:0000250|UniProtKB:P52046};
DE   Flags: Fragment;
GN   Name=crt; Synonyms=ech;
OS   Clostridioides difficile (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=1496;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E;
RX   PubMed=8001771; DOI=10.1111/j.1574-6968.1994.tb07262.x;
RA   Mullany P., Clayton C.L., Pallen M.J., Slone R., Al-Saleh A.,
RA   Tabaqchali S.;
RT   "Genes encoding homologues of three consecutive enzymes in the
RT   butyrate/butanol-producing pathway of Clostridium acetobutylicum are
RT   clustered on the Clostridium difficile chromosome.";
RL   FEMS Microbiol. Lett. 124:61-67(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain (2E)-
CC         enoyl-CoA + H2O; Xref=Rhea:RHEA:52664, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:87488, ChEBI:CHEBI:136760; EC=4.2.1.150;
CC         Evidence={ECO:0000250|UniProtKB:P52046};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism.
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; X79899; CAA56274.1; -; Genomic_DNA.
DR   PIR; I40678; I40678.
DR   AlphaFoldDB; P45361; -.
DR   SMR; P45361; -.
DR   UniPathway; UPA00863; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.12.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR   InterPro; IPR014748; Enoyl-CoA_hydra_C.
DR   Pfam; PF00378; ECH_1; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
PE   3: Inferred from homology;
KW   Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           <1..155
FT                   /note="Short-chain-enoyl-CoA hydratase"
FT                   /id="PRO_0000109318"
FT   NON_TER         1
SQ   SEQUENCE   155 AA;  16581 MW;  D1AF52EEEC666501 CRC64;
     NSKKVVIAAV NGFALGGCEL AMACDIRIAS AKAKFGQPEV TLGITPGYGG TQRLTRLVGM
     AKAKELIFTG QVIKADEAEK IGLVNRVVEP DILIEEVEKL AKIIAKNAQL AVRYSKEAIQ
     LGAQTDINTG IDIESNLFGL CFSTKDQKEG IVSFR