ID   CRT_CLOPA               Reviewed;          22 AA.
AC   P81357;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   02-JUN-2021, entry version 50.
DE   RecName: Full=Short-chain-enoyl-CoA hydratase {ECO:0000250|UniProtKB:P52046};
DE            EC=4.2.1.150 {ECO:0000250|UniProtKB:P52046};
DE   AltName: Full=3-hydroxybutyryl-CoA dehydratase {ECO:0000250|UniProtKB:P52046};
DE   AltName: Full=CP 24;
DE   AltName: Full=Crotonase {ECO:0000250|UniProtKB:P52046};
DE   Flags: Fragment;
GN   Name=crt;
OS   Clostridium pasteurianum.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1501;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 6013 / DSM 525 / NCIB 9486 / VKM B-1774 / W5;
RX   PubMed=9629918; DOI=10.1002/elps.1150190533;
RA   Flengsrud R., Skjeldal L.;
RT   "Two-dimensional gel electrophoresis separation and N-terminal sequence
RT   analysis of proteins from Clostridium pasteurianum W5.";
RL   Electrophoresis 19:802-806(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain (2E)-
CC         enoyl-CoA + H2O; Xref=Rhea:RHEA:52664, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:87488, ChEBI:CHEBI:136760; EC=4.2.1.150;
CC         Evidence={ECO:0000250|UniProtKB:P52046};
CC   -!- PATHWAY: Lipid metabolism; butanoate metabolism.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this unknown protein
CC       is 5.2, its MW is 30.9 kDa.
CC   -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC       {ECO:0000305}.
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DR   UniPathway; UPA00863; -.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019605; P:butyrate metabolic process; IEA:UniProtKB-UniPathway.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Fatty acid metabolism; Lipid metabolism; Lyase.
FT   CHAIN           1..>22
FT                   /note="Short-chain-enoyl-CoA hydratase"
FT                   /id="PRO_0000109319"
FT   VARIANT         20
FT                   /note="N -> D"
FT   NON_TER         22
SQ   SEQUENCE   22 AA;  2298 MW;  95CD79FAD3FE7CDB CRC64;
     MGFNGGILEG EEXKAVVTIN XP