ID   CRT_PLABE               Reviewed;         425 AA.
AC   Q9GSD8;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Putative chloroquine resistance transporter;
DE   AltName: Full=Probable transporter cg10;
DE            Short=pbcg10;
DE   AltName: Full=pfcrt homolog;
GN   Name=CG10;
OS   Plasmodium berghei.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX   NCBI_TaxID=5821;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11343215; DOI=10.1086/320707;
RA   Nomura T., Carlton J.M.-R., Baird J.K., del Portillo H.A., Fryauff D.J.,
RA   Rathore D., Fidock D.A., Su X.-Z., Collins W.E., McCutchan T.F.,
RA   Wootton J.C., Wellems T.E.;
RT   "Evidence for different mechanisms of chloroquine resistance in 2
RT   Plasmodium species that cause human malaria.";
RL   J. Infect. Dis. 183:1653-1661(2001).
CC   -!- FUNCTION: May regulate endogenous transporter. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC       Note=Localizes to the parasite digestive vacuole, the site of
CC       chloroquine action. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CRT-like transporter family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF314645; AAG27734.1; -; mRNA.
DR   AlphaFoldDB; Q9GSD8; -.
DR   SMR; Q9GSD8; -.
DR   VEuPathDB; PlasmoDB:PBANKA_1219500; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR013936; CRT-like.
DR   InterPro; IPR017258; Transprt_Chloroquine.
DR   PANTHER; PTHR31326; PTHR31326; 1.
DR   Pfam; PF08627; CRT-like; 1.
DR   PIRSF; PIRSF037671; Transprt_Chloroquine_res; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Vacuole.
FT   CHAIN           1..425
FT                   /note="Putative chloroquine resistance transporter"
FT                   /id="PRO_0000385356"
FT   TOPO_DOM        1..56
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        78..88
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..126
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        148..157
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        179..181
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        203..210
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        232..249
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        250..270
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        271..318
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        319..339
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        340..347
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        348..368
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        369..378
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        400..425
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        86
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   425 AA;  48937 MW;  0C6973B42B033CDE CRC64;
     MTGIKKGKNK KKNMKNDDRY KELDSLITNG SEIGNNSGRS CVKRFFKIIG NEMKNNVYVY
     LLSILYLCVC VMNKVFAKRT LNKMGNYSFV TSETHNIICI IVFQLLYFIY RKTSSSSVYK
     NESQKNFGWQ FFLISLLDAS TVIISMIGLT RTTGNIQSFI MQLIIPVNMY FWFMFLGYRY
     HLFNYLGAFI ILITIAVVET FLSFETQGEN SIIFNLIMIS AFNTLSFSNM TREVVFKKHK
     INILRLNAMV VLFQFFTSLL VLPVYNIPFL KEIYMPFSEM STNINNGLRC LFYGENTIVE
     NCGVGMVKMC DNCEGAWKTF ITFSFFNICD NLLACYIIDK FSTMTYTIVS CIQGPAITIA
     YYFKFLAGDA VRKPRILDFL TLFGYLFGTI IYRIGNIILE KKQVIKSQNS NDSEAELTSI
     ETSRA