CRT_PLAF7
ID CRT_PLAF7 Reviewed; 424 AA.
AC Q8IBZ9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Putative chloroquine resistance transporter;
DE AltName: Full=PfCRT;
DE AltName: Full=Probable transporter cg10;
GN Name=CG10; ORFNames=MAL7P1.27;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
CC -!- FUNCTION: May regulate endogenous transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC Note=Localizes to the parasite digestive vacuole, the site of
CC chloroquine action. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CRT-like transporter family. {ECO:0000305}.
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DR EMBL; AL844506; CAD50842.1; -; Genomic_DNA.
DR RefSeq; XP_001349004.1; XM_001348968.1.
DR AlphaFoldDB; Q8IBZ9; -.
DR SMR; Q8IBZ9; -.
DR STRING; 5833.MAL7P1.27; -.
DR SwissPalm; Q8IBZ9; -.
DR PRIDE; Q8IBZ9; -.
DR EnsemblProtists; CAD50842; CAD50842; PF3D7_0709000.
DR GeneID; 2655199; -.
DR KEGG; pfa:PF3D7_0709000; -.
DR VEuPathDB; PlasmoDB:PF3D7_0709000; -.
DR HOGENOM; CLU_648097_0_0_1; -.
DR InParanoid; Q8IBZ9; -.
DR OMA; IWPALMI; -.
DR PhylomeDB; Q8IBZ9; -.
DR Proteomes; UP000001450; Chromosome 7.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:GeneDB.
DR GO; GO:0020020; C:food vacuole; IDA:GeneDB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; TAS:GeneDB.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IMP:GeneDB.
DR GO; GO:0034635; P:glutathione transport; IDA:GeneDB.
DR InterPro; IPR013936; CRT-like.
DR InterPro; IPR017258; Transprt_Chloroquine.
DR PANTHER; PTHR31326; PTHR31326; 1.
DR Pfam; PF08627; CRT-like; 1.
DR PIRSF; PIRSF037671; Transprt_Chloroquine_res; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..424
FT /note="Putative chloroquine resistance transporter"
FT /id="PRO_0000385358"
FT TOPO_DOM 1..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..90
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..127
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..154
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..209
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..248
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..317
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..346
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 368..377
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 424 AA; 48675 MW; EC642763C5C73732 CRC64;
MKFASKKNNQ KNSSKNDERY RELDNLVQEG NGSRLGGGSC LGKCAHVFKL IFKEIKDNIF
IYILSIIYLS VCVMNKIFAK RTLNKIGNYS FVTSETHNFI CMIMFFIVYS LFGNKKGNSK
ERHRSFNLQF FAISMLDACS VILAFIGLTR TTGNIQSFVL QLSIPINMFF CFLILRYRYH
LYNYLGAVII VVTIALVEMK LSFETQEENS IIFNLVLISA LIPVCFSNMT REIVFKKYKI
DILRLNAMVS FFQLFTSCLI LPVYTLPFLK QLHLPYNEIW TNIKNGFACL FLGRNTVVEN
CGLGMAKLCD DCDGAWKTFA LFSFFNICDN LITSYIIDKF STMTYTIVSC IQGPAIAIAY
YFKFLAGDVV REPRLLDFVT LFGYLFGSII YRVGNIILER KKMRNEENED SEGELTNVDS
IITQ
