ID   CRT_PLAKN               Reviewed;         424 AA.
AC   Q9GSD7;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Putative chloroquine resistance transporter;
DE   AltName: Full=Probable transporter cg10;
DE            Short=pkcg10;
DE   AltName: Full=pfcrt homolog;
GN   Name=CG10;
OS   Plasmodium knowlesi.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Plasmodium).
OX   NCBI_TaxID=5850;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11343215; DOI=10.1086/320707;
RA   Nomura T., Carlton J.M.-R., Baird J.K., del Portillo H.A., Fryauff D.J.,
RA   Rathore D., Fidock D.A., Su X.-Z., Collins W.E., McCutchan T.F.,
RA   Wootton J.C., Wellems T.E.;
RT   "Evidence for different mechanisms of chloroquine resistance in 2
RT   Plasmodium species that cause human malaria.";
RL   J. Infect. Dis. 183:1653-1661(2001).
CC   -!- FUNCTION: May regulate endogenous transporter. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC       Note=Localizes to the parasite digestive vacuole, the site of
CC       chloroquine action. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CRT-like transporter family. {ECO:0000305}.
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DR   EMBL; AF314646; AAG27735.1; -; mRNA.
DR   AlphaFoldDB; Q9GSD7; -.
DR   SMR; Q9GSD7; -.
DR   VEuPathDB; PlasmoDB:PKA1H_010012400; -.
DR   VEuPathDB; PlasmoDB:PKNH_0107600; -.
DR   VEuPathDB; PlasmoDB:PKNOH_S01015900; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR013936; CRT-like.
DR   InterPro; IPR017258; Transprt_Chloroquine.
DR   PANTHER; PTHR31326; PTHR31326; 1.
DR   Pfam; PF08627; CRT-like; 1.
DR   PIRSF; PIRSF037671; Transprt_Chloroquine_res; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Vacuole.
FT   CHAIN           1..424
FT                   /note="Putative chloroquine resistance transporter"
FT                   /id="PRO_0000385359"
FT   TOPO_DOM        1..57
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        79..89
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        111..124
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        146..153
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        175..179
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        180..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        201..208
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        230..246
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        247..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        268..316
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        317..337
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        338..345
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        367..376
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        377..397
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        398..424
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        87
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   424 AA;  48673 MW;  E3F2872D08898753 CRC64;
     MKILKKKKKG NQQIVPDERY RELDSHAPNE NEIADEAPMS RKILYYLKLV YHEIRENITI
     YLLIILYLCV CVMNKIMAKR TLKKIGNYSF VTSETHNTIC MVVFFSLYFI FGRRVTSAKE
     RHQNFGLQFL LISLLDACSV IIAFIGLTRT TGNIQSFVMQ LSIPINMFFC FLILRYRYHL
     FNYVGASIIV LTIAIVEFIL SFETQEENSI VFNLVLIASL IPMSFSNMTR EIVFKKYKIN
     ILRLNAVVSF FQIFTSCLML PMYTLPFLKQ INLPFSEIGT NIKNGFRCLI LGQNTIVENC
     GLGMAKMCDD CEGAWKTFLA YSFFNICDNL ITSFIIDKFS TMTYTIVSCI QGPAIAIAYY
     FKFLAGDAVM KPRVLDFVTL FGYLFGSIIY RVGNIILEKK KMMEAGNDDD SEGELTNAES
     IITQ