CRT_PLAVS
ID CRT_PLAVS Reviewed; 424 AA.
AC Q9GSD3;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Putative chloroquine resistance transporter;
DE AltName: Full=Probable transporter cg10;
DE Short=pvcg10;
DE AltName: Full=pfcrt ortholog;
DE Short=pvcrt-o;
GN Name=CG10; Synonyms=CRT-O; ORFNames=PVX_087980;
OS Plasmodium vivax (strain Salvador I).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Plasmodium).
OX NCBI_TaxID=126793;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS HIS-34; LEU-38 AND PHE-384.
RC STRAIN=Salvador I;
RX PubMed=11343215; DOI=10.1086/320707;
RA Nomura T., Carlton J.M.-R., Baird J.K., del Portillo H.A., Fryauff D.J.,
RA Rathore D., Fidock D.A., Su X.-Z., Collins W.E., McCutchan T.F.,
RA Wootton J.C., Wellems T.E.;
RT "Evidence for different mechanisms of chloroquine resistance in 2
RT Plasmodium species that cause human malaria.";
RL J. Infect. Dis. 183:1653-1661(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MCQS2;
RA Orjuela-Sanchez P., del Portillo H.A.;
RT "Primary sequences of pvmdr1 and pvcrt-o genes from chloroquine sensitive
RT and chloroquine resistance Brazilian isolates.";
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Salvador I;
RX PubMed=18843361; DOI=10.1038/nature07327;
RA Carlton J.M., Adams J.H., Silva J.C., Bidwell S.L., Lorenzi H., Caler E.,
RA Crabtree J., Angiuoli S.V., Merino E.F., Amedeo P., Cheng Q.,
RA Coulson R.M.R., Crabb B.S., del Portillo H.A., Essien K., Feldblyum T.V.,
RA Fernandez-Becerra C., Gilson P.R., Gueye A.H., Guo X., Kang'a S.,
RA Kooij T.W.A., Korsinczky M., Meyer E.V.-S., Nene V., Paulsen I., White O.,
RA Ralph S.A., Ren Q., Sargeant T.J., Salzberg S.L., Stoeckert C.J.,
RA Sullivan S.A., Yamamoto M.M., Hoffman S.L., Wortman J.R., Gardner M.J.,
RA Galinski M.R., Barnwell J.W., Fraser-Liggett C.M.;
RT "Comparative genomics of the neglected human malaria parasite Plasmodium
RT vivax.";
RL Nature 455:757-763(2008).
CC -!- FUNCTION: May regulate endogenous transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC Note=Localizes to the parasite digestive vacuole, the site of
CC chloroquine action. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CRT-like transporter family. {ECO:0000305}.
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DR EMBL; AF314649; AAG27739.1; -; Genomic_DNA.
DR EMBL; EU333970; ACA35014.1; -; Genomic_DNA.
DR EMBL; AAKM01000014; EDL43730.1; -; Genomic_DNA.
DR RefSeq; XP_001613457.1; XM_001613407.1.
DR AlphaFoldDB; Q9GSD3; -.
DR SMR; Q9GSD3; -.
DR STRING; 126793.Q9GSD3; -.
DR EnsemblProtists; EDL43730; EDL43730; PVX_087980.
DR GeneID; 5472721; -.
DR KEGG; pvx:PVX_087980; -.
DR VEuPathDB; PlasmoDB:PVX_087980; -.
DR InParanoid; Q9GSD3; -.
DR OMA; IWPALMI; -.
DR PhylomeDB; Q9GSD3; -.
DR Proteomes; UP000008333; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042910; F:xenobiotic transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR013936; CRT-like.
DR InterPro; IPR017258; Transprt_Chloroquine.
DR PANTHER; PTHR31326; PTHR31326; 1.
DR Pfam; PF08627; CRT-like; 1.
DR PIRSF; PIRSF037671; Transprt_Chloroquine_res; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..424
FT /note="Putative chloroquine resistance transporter"
FT /id="PRO_0000385360"
FT TOPO_DOM 1..57
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 79..89
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..124
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..153
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..179
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 201..208
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..246
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..316
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338..345
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 346..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 367..376
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 34
FT /note="Q -> H"
FT /evidence="ECO:0000269|PubMed:11343215"
FT VARIANT 38
FT /note="P -> L"
FT /evidence="ECO:0000269|PubMed:11343215"
FT VARIANT 384
FT /note="L -> F"
FT /evidence="ECO:0000269|PubMed:11343215"
SQ SEQUENCE 424 AA; 48670 MW; EB54A287C401BFD9 CRC64;
MTILKKKKKG SPQITPDERY RELDSHAQNE SEIQEDVPIS RKIANFLKLA YNEIRENISI
YLLIIVYLCV CVMNKLLAKR TLKKIGNYSF VTSETHNCIC MVVFFALYFM FGRRVMSAKE
RHRNFGVQFL LISLLDACSV IIAFIGLTRT TGNIQSFVMQ LSIPINMFFC FLILRYRYHL
FNYVGAFIIV VTIAVVEFML SFETQEENSI VFNLVLIASL IPLSFSNMTR EIVFKKYKIN
ILRLNAVVSF FQIFTSCLML PMYTLPFLKQ INLPFSEIGT NIKNGFRCLF LGQNTIVENC
GLGMSKMCDD CEGAWKTFIA YSFFNICDNL ITSFIIEKFS TMTYTIVSCI QGPAIAIAYY
FKFLAGDAVM QPRMLDFVTL FGYLFGSIIY RIGNIILEKK RMMEAGNDDD SEGELTNADS
IITH
