ID   CRU1_RAPSA              Reviewed;         479 AA.
AC   Q02498;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Cruciferin PGCRURSE5;
DE   AltName: Full=11S globulin;
DE   AltName: Full=12S storage protein;
DE   Contains:
DE     RecName: Full=Cruciferin PGCRURSE5 alpha chain;
DE   Contains:
DE     RecName: Full=Cruciferin PGCRURSE5 beta chain;
DE   Flags: Precursor;
GN   Name=CRURS;
OS   Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX   NCBI_TaxID=3726;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Saxa Knacker;
RX   PubMed=1421150; DOI=10.1007/bf00040606;
RA   Depigny-This D., Raynal M., Aspart L., Delseny M., Grellet F.;
RT   "The cruciferin gene family in radish.";
RL   Plant Mol. Biol. 20:467-479(1992).
CC   -!- FUNCTION: This is a seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; X59808; CAA42478.1; -; Genomic_DNA.
DR   PIR; S26223; S26223.
DR   AlphaFoldDB; Q02498; -.
DR   SMR; Q02498; -.
DR   Proteomes; UP000504610; Unplaced.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   GO; GO:0010431; P:seed maturation; IEA:UniProt.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR022379; 11S_seedstore_CS.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Phosphoprotein; Reference proteome; Seed storage protein;
KW   Signal; Storage protein.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..289
FT                   /note="Cruciferin PGCRURSE5 alpha chain"
FT                   /id="PRO_0000032040"
FT   CHAIN           290..479
FT                   /note="Cruciferin PGCRURSE5 beta chain"
FT                   /id="PRO_0000032041"
FT   DOMAIN          42..241
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          302..451
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          117..144
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          196..219
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          271..291
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        119..143
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         116
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P15455"
FT   MOD_RES         415
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P15456"
FT   MOD_RES         440
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P15456"
FT   DISULFID        37..70
FT                   /evidence="ECO:0000250"
FT   DISULFID        113..296
FT                   /note="Interchain (between alpha and beta chains)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   479 AA;  53256 MW;  4E06960C0974E710 CRC64;
     MVKLAHLLVA TFGVLLVLNG CLARQSLGVP PQLGNACNLD NLDVLQPTET IKSEAGRLEY
     WDHNHPQLRC AGVSVSRLII EQGGLYLPTF FSSPKIAYVV QGMGISGRVV PGCAETFMDS
     QPMQGQGQQG QQGQQGQQQQ GFRDMHQKVE HVRHGDVIAI TAGSAHWIYN TGDQPLVIVC
     LLDIANYQNQ LDRNPRTFRL AGNNPQGGSH QQQQQQQQNM LSGFDPQVLA QALKMQLRLA
     QELQNQQDNR GNIVRVKGPF QVVRPPLRQQ YESEQWRHPR GPPQSPQDNG LEETICSMRT
     HENIDDPARA DVYKPNLGRV TSVNSYTLPI LQYIRLSATR GILQGNAMVL PKYNMNANEI
     LYCTQGQARI QVVNDNGQNV LDQQVQKGQL VVIPQGFAYV VQSHGNNFEW ISFKTNANAM
     VSTLAGRTSA LRALPLEVIT NAFQISLEEA RRIKFNTPET TLTHARGGQP QLIEEIVEA