ID   CRU3_BRANA              Reviewed;         509 AA.
AC   P33525;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Cruciferin CRU1;
DE   AltName: Full=11S globulin;
DE   AltName: Full=12S storage protein;
DE   Contains:
DE     RecName: Full=Cruciferin CRU1 alpha chain;
DE   Contains:
DE     RecName: Full=Cruciferin CRU1 beta chain;
DE   Flags: Precursor;
GN   Name=CRU1;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1421158; DOI=10.1007/bf00040615;
RA   Roedin J., Sjoedahl S., Josefsson L.-G., Rask L.;
RT   "Characterization of a Brassica napus gene encoding a cruciferin subunit:
RT   estimation of sizes of cruciferin gene families.";
RL   Plant Mol. Biol. 20:559-563(1992).
CC   -!- FUNCTION: This is a seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; X62120; CAA44042.1; -; Genomic_DNA.
DR   AlphaFoldDB; P33525; -.
DR   SMR; P33525; -.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   GO; GO:0010431; P:seed maturation; IEA:UniProt.
DR   Gene3D; 2.60.120.10; -; 3.
DR   InterPro; IPR022379; 11S_seedstore_CS.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Phosphoprotein; Seed storage protein; Signal;
KW   Storage protein.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..319
FT                   /note="Cruciferin CRU1 alpha chain"
FT                   /id="PRO_0000032036"
FT   CHAIN           320..509
FT                   /note="Cruciferin CRU1 beta chain"
FT                   /id="PRO_0000032037"
FT   DOMAIN          42..271
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          332..481
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          119..175
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          230..249
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          301..321
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        119..174
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         53
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96318"
FT   MOD_RES         97
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96318"
FT   MOD_RES         116
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96318"
FT   MOD_RES         352
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96318"
FT   MOD_RES         445
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96318"
FT   MOD_RES         487
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96318"
FT   DISULFID        37..70
FT                   /evidence="ECO:0000250"
FT   DISULFID        113..326
FT                   /note="Interchain (between alpha and beta chains)"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   509 AA;  56503 MW;  5939845DF2A08695 CRC64;
     MVKVPHLLVA TFGVLLVLNG CLARQSLGVP PQLGNACNLD NLDVLQPTET IKSEAGRVEY
     WDHNNPQIRC AGVSVSRVII EQGGLYLPTF FSSPKISYVV QGMGISGRVV PGCAETFMDS
     QPMQGQQQGQ PWQGQQGQQG QQGQQGQQGQ QGQQGQQGQQ GQQGQQGQQQ QGFRDMHQKV
     EHVRHGDIIA ITAGSSHWIY NTGDQPLVII CLLDIANYQN QLDRNPRTFR LAGNNPQGGS
     QQQQQQQQNM LSGFDPQVLA QALKIDVRLA QELQNQQDSR GNIVRVKGPF QVVRPPLRQP
     YESEQWRHPR GPPQSPQDNG LEETICSMRT HENIDDPARA DVYKPNLGRV TSVNSYTLPI
     LQYIRLSATR GILQGNAMVL PKYNMNANEI LYCTQGQARI QVVNDNGQNV LDQQVQKGQL
     VVIPQGFAYV VQSHQNNFEW ISFKTNANAM VSTLAGRTSA LRALPLEVIT NAFQISLEEA
     RRIKFNTLET TLTRARGGQP QLIEEIVEA