ID   CRUF_HALJT              Reviewed;         293 AA.
AC   A0A0A1GNF2; M0L9D2;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   04-FEB-2015, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=Bisanhydrobacterioruberin hydratase {ECO:0000303|PubMed:25712483};
DE            EC=4.2.1.161 {ECO:0000269|PubMed:25712483};
GN   Name=cruF {ECO:0000303|PubMed:25712483};
GN   Synonyms=c0505 {ECO:0000303|PubMed:25712483};
GN   ORFNames=C444_12927 {ECO:0000312|EMBL:EMA29703.1};
OS   Haloarcula japonica (strain ATCC 49778 / DSM 6131 / JCM 7785 / NBRC 101032
OS   / NCIMB 13157 / TR-1).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Haloarcula.
OX   NCBI_TaxID=1227453;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 49778 / DSM 6131 / JCM 7785 / NBRC 101032 / NCIMB 13157 / TR-1;
RX   PubMed=25712483; DOI=10.1128/jb.02523-14;
RA   Yang Y., Yatsunami R., Ando A., Miyoko N., Fukui T., Takaichi S.,
RA   Nakamura S.;
RT   "Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from
RT   lycopene in the extremely halophilic archaeon Haloarcula japonica.";
RL   J. Bacteriol. 197:1614-1623(2015).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49778 / DSM 6131 / JCM 7785 / NBRC 101032 / NCIMB 13157 / TR-1;
RX   PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA   Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA   Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT   "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT   strategies for static and dynamic osmo-response.";
RL   PLoS Genet. 10:E1004784-E1004784(2014).
CC   -!- FUNCTION: Involved in the biosynthesis of the acyclic C50 carotenoid
CC       bacterioruberin (BR). Catalyzes the reaction that introduces hydroxyl
CC       groups to C3'' and C3''' of bisanhydrobacterioruberin (BABR) to
CC       generate BR. {ECO:0000269|PubMed:25712483}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=bacterioruberin = bisanhydrobacterioruberin + 2 H2O;
CC         Xref=Rhea:RHEA:38375, ChEBI:CHEBI:15377, ChEBI:CHEBI:49388,
CC         ChEBI:CHEBI:87121; EC=4.2.1.161;
CC         Evidence={ECO:0000269|PubMed:25712483};
CC   -!- PATHWAY: Carotenoid biosynthesis. {ECO:0000305|PubMed:25712483}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene accumulate BABR.
CC       {ECO:0000269|PubMed:25712483}.
CC   -!- SIMILARITY: Belongs to the BABR hydratase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; LC008544; BAP82511.1; -; Genomic_DNA.
DR   EMBL; AOLY01000037; EMA29703.1; -; Genomic_DNA.
DR   RefSeq; WP_004593286.1; NZ_AOLY01000037.1.
DR   AlphaFoldDB; A0A0A1GNF2; -.
DR   STRING; 1227453.C444_12927; -.
DR   EnsemblBacteria; EMA29703; EMA29703; C444_12927.
DR   KEGG; ag:BAP82511; -.
DR   PATRIC; fig|1227453.3.peg.2545; -.
DR   eggNOG; arCOG02835; Archaea.
DR   OrthoDB; 77334at2157; -.
DR   BioCyc; MetaCyc:MON-20364; -.
DR   BRENDA; 4.2.1.161; 13658.
DR   Proteomes; UP000011524; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016836; F:hydro-lyase activity; IDA:UniProtKB.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IMP:UniProtKB.
DR   InterPro; IPR017823; CruF.
DR   InterPro; IPR007354; CruF-like.
DR   PANTHER; PTHR39419; PTHR39419; 1.
DR   Pfam; PF04240; Caroten_synth; 1.
DR   TIGRFAMs; TIGR03460; crt_membr_arch; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Lyase; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..293
FT                   /note="Bisanhydrobacterioruberin hydratase"
FT                   /id="PRO_0000435600"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   293 AA;  32402 MW;  E1BAC8BF9526E547 CRC64;
     MGSGKDRTLA GWTLPETKTD ATAQFDRFVT ENRFTIAVVF PLVGAVTLLA SAEGLLPDPL
     AFNPYFVLFG TFVMRLPLVA GIFPLVDRRA GLALVALTLY SYGIELVGVR TGWPYGEFTY
     GVDLGPMLLG DVPFGLPVFF FPLVLNAYLL VLLLLGNRAA STTVRLLSTL ATVMLVDLVL
     DPGAVAIGFW IYEMPQFYGV PWQNYAGWLL SGSVAVLLFD FGFDRAGLRR RLRDCPFMLD
     DLVSFVLLWG GINLFYTNWV PFGLAALLGA GLLWTDRFDF DLSETRLGRA VWR