ID   CRVP2_NAJAT             Reviewed;         238 AA.
AC   Q7ZZN8;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Cysteine-rich venom protein natrin-2;
DE   AltName: Full=Cysteine-rich venom protein 2;
DE   AltName: Full=NA-CRVP2;
DE   AltName: Full=Protein G2b;
DE   Flags: Precursor;
OS   Naja atra (Chinese cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 20-52.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=14529736; DOI=10.1016/s0041-0101(03)00234-4;
RA   Jin Y., Lu Q., Zhou X., Zhu S., Li R., Wang W., Xiong Y.;
RT   "Purification and cloning of cysteine-rich proteins from Trimeresurus
RT   jerdonii and Naja atra venoms.";
RL   Toxicon 42:539-547(2003).
RN   [2]
RP   PROTEIN SEQUENCE OF 26-45, AMINO-ACID COMPOSITION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=15581679; DOI=10.1016/j.toxicon.2004.09.002;
RA   Chang L.-S., Liou J.-C., Lin S.-R., Cheng Y.-C.;
RT   "Purification and characterization of Taiwan cobra venom proteins with weak
RT   toxicity.";
RL   Toxicon 45:21-25(2005).
CC   -!- FUNCTION: Inhibits carbachol-induced muscle contraction and weakly
CC       blocks muscle contraction evoked by potassium.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: Mass=23637; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:15581679};
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   EMBL; AY261468; AAP20603.1; -; mRNA.
DR   AlphaFoldDB; Q7ZZN8; -.
DR   SMR; Q7ZZN8; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd05383; CAP_CRISP; 1.
DR   Gene3D; 1.10.10.740; -; 1.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR042076; Crisp-like_dom.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR013871; Cysteine_rich_secretory.
DR   InterPro; IPR034117; SCP_CRISP.
DR   InterPro; IPR003582; ShKT_dom.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   Pfam; PF08562; Crisp; 1.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
DR   PROSITE; PS51670; SHKT; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:14529736"
FT   CHAIN           20..238
FT                   /note="Cysteine-rich venom protein natrin-2"
FT                   /id="PRO_0000006280"
FT   DOMAIN          38..164
FT                   /note="SCP"
FT   DOMAIN          200..233
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        75..153
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        92..165
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        148..162
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        184..191
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        187..196
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        200..233
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        209..227
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        218..231
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
SQ   SEQUENCE   238 AA;  26246 MW;  5A6DA4EFA227256C CRC64;
     MIAFIVLLSL AAVLQQSSGT VDFASESSNK RENQKQIVDK HNALRRSVRP TARNMLQMEW
     NSNAAQNAKR WADRCSFAHS PPHLRTVGKI GCGENLFMSS QPYAWSRVIQ SWYDENKKFV
     YGVGANPPGS VIGHYTQIVW YNSHLLGCGA AKCSSSKYLY VCQYCPTGNI IGSIATPYKS
     GPPCGDCPSA CVNGLCTNPC KHHNVFSNCQ SLAKQNACQT EWMKSKCAAS CFCRTEII