ID   CRVP2_NAJKA             Reviewed;         238 AA.
AC   P84808; B5THG9;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 2.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Cysteine-rich venom protein kaouthin-2;
DE   AltName: Full=Cysteine-rich venom protein 23;
DE            Short=CRVP-23k;
DE   Flags: Precursor;
OS   Naja kaouthia (Monocled cobra) (Naja siamensis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8649;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 26-90 AND 202-226.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=19106157; DOI=10.1093/jb/mvn174;
RA   Matsunaga Y., Yamazaki Y., Hyodo F., Sugiyama Y., Nozaki M., Morita T.;
RT   "Structural divergence of cysteine-rich secretory proteins in snake
RT   venoms.";
RL   J. Biochem. 145:365-375(2009).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 26-38, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Venom {ECO:0000269|PubMed:15670767};
RX   PubMed=15670767; DOI=10.1016/j.bbrc.2004.12.154;
RA   Osipov A.V., Levashov M.Y., Tsetlin V.I., Utkin Y.N.;
RT   "Cobra venom contains a pool of cysteine-rich secretory proteins.";
RL   Biochem. Biophys. Res. Commun. 328:177-182(2005).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15670767}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:15670767}.
CC   -!- MASS SPECTROMETRY: Mass=23621; Method=MALDI; Note=It is not clear why
CC       there are two peaks for this protein.;
CC       Evidence={ECO:0000269|PubMed:15670767};
CC   -!- MASS SPECTROMETRY: Mass=23704; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15670767};
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU938340; ACH73168.1; -; mRNA.
DR   AlphaFoldDB; P84808; -.
DR   SMR; P84808; -.
DR   PRIDE; P84808; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IDA:UniProtKB.
DR   CDD; cd05383; CAP_CRISP; 1.
DR   Gene3D; 1.10.10.740; -; 1.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR042076; Crisp-like_dom.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR013871; Cysteine_rich_secretory.
DR   InterPro; IPR034117; SCP_CRISP.
DR   InterPro; IPR003582; ShKT_dom.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   Pfam; PF08562; Crisp; 1.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
DR   PROSITE; PS51670; SHKT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000250"
FT   PROPEP          20..25
FT                   /evidence="ECO:0000269|PubMed:15670767,
FT                   ECO:0000269|PubMed:19106157"
FT                   /id="PRO_0000422143"
FT   CHAIN           26..238
FT                   /note="Cysteine-rich venom protein kaouthin-2"
FT                   /id="PRO_5000399106"
FT   DOMAIN          38..164
FT                   /note="SCP"
FT   DOMAIN          200..233
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        75..153
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        92..165
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        148..162
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        184..191
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        187..196
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        200..233
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        209..227
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        218..231
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
SQ   SEQUENCE   238 AA;  26216 MW;  0B6CB4FEA3272570 CRC64;
     MIAFIVLLSL AAVLQQSSGT VDFASESSNK RENQKQIVDK HNALRRSVRP TARNMLQMEW
     NSNAAQNAKR WADRCSFAHS PPHLRTVGKI GCGENLFMSS QPYAWSRVIQ SWYDENKKFV
     YGVGANPPGS VIGHYTQIVW YNSHLLGCGA AKCSSSKYLY VCQYCPAGNI IGSIATPYKS
     GPPCGDCPSA CVNGLCTNPC KHHNVFSNCQ SLAKQNACQT EWMKSKCAAS CFCRTEII