ID   CRVP_ECHCO              Reviewed;         220 AA.
AC   P0DMT4;
DT   01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-2015, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Cysteine-rich venom protein;
DE            Short=CRVP;
DE   AltName: Full=ECO_00025;
OS   Echis coloratus (Carpet viper).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Viperinae; Echis.
OX   NCBI_TaxID=64175;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=23747272; DOI=10.1016/j.toxicon.2013.05.017;
RA   Conlon J.M., Attoub S., Arafat H., Mechkarska M., Casewell N.R.,
RA   Harrison R.A., Calvete J.J.;
RT   "Cytotoxic activities of [Ser49]phospholipase A(2) from the venom of the
RT   saw-scaled vipers Echis ocellatus, Echis pyramidum leakeyi, Echis carinatus
RT   sochureki, and Echis coloratus.";
RL   Toxicon 71:96-104(2013).
CC   -!- FUNCTION: Blocks contraction of smooth muscle elicited by high
CC       potassium-induced depolarization, but does not block caffeine-
CC       stimulated contraction. May target voltage-gated calcium channels in
CC       smooth muscle. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=24682; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:23747272};
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   AlphaFoldDB; P0DMT4; -.
DR   SMR; P0DMT4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd05383; CAP_CRISP; 1.
DR   Gene3D; 1.10.10.740; -; 1.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR042076; Crisp-like_dom.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR013871; Cysteine_rich_secretory.
DR   InterPro; IPR034117; SCP_CRISP.
DR   InterPro; IPR003582; ShKT_dom.
DR   InterPro; IPR002413; V5_allergen-like.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   Pfam; PF08562; Crisp; 1.
DR   PRINTS; PR00838; V5ALLERGEN.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
DR   PROSITE; PS51670; SHKT; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Toxin.
FT   CHAIN           1..220
FT                   /note="Cysteine-rich venom protein"
FT                   /evidence="ECO:0000250|UniProtKB:B7FDI0"
FT                   /id="PRO_0000432599"
FT   DOMAIN          20..147
FT                   /note="SCP"
FT                   /evidence="ECO:0000250|UniProtKB:B7FDI0"
FT   DOMAIN          183..215
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        56..134
FT                   /evidence="ECO:0000250"
FT   DISULFID        73..148
FT                   /evidence="ECO:0000250"
FT   DISULFID        129..145
FT                   /evidence="ECO:0000250"
FT   DISULFID        167..174
FT                   /evidence="ECO:0000250"
FT   DISULFID        170..179
FT                   /evidence="ECO:0000250"
FT   DISULFID        183..215
FT                   /evidence="ECO:0000250"
FT   DISULFID        192..209
FT                   /evidence="ECO:0000250"
FT   DISULFID        200..213
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   220 AA;  24699 MW;  7F454E35DDF7064E CRC64;
     NVDFDSESPR KPEIQNEIID LHNSLRRSVN PTASNMLRME WYPEAAANAE RWAFRCTLNH
     SPRDSRVIDG IKCGENIYMS PYPIKWTAII HKWHDEKKNF VYGIGASPAN AVIGHYTQIV
     WYKSYRGGCA AAYCPSSAYK YFYVCQYCPA GNIIGKTATP YKSGPPCGDC PSACDNGLCT
     NPCTREDEFI NCNDLVKQGC QTDYLKSNCA ASCFCHSEIK