ID   CRVP_RHATT              Reviewed;         237 AA.
AC   Q8JGT9; Q2XXP6;
DT   29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Cysteine-rich venom protein tigrin;
DE            Short=CRVP;
DE   AltName: Full=Cysteine-rich secretory protein RHA1;
DE            Short=CRISP-RHA1;
DE   Flags: Precursor;
OS   Rhabdophis tigrinus tigrinus (Tiger keelback snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Colubridae; Natricinae; Rhabdophis.
OX   NCBI_TaxID=193080;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-56; 142-157; 161-171;
RP   205-218; 220-221 AND 223-235, AND FUNCTION.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=12047379; DOI=10.1046/j.1432-1033.2002.02940.x;
RA   Yamazaki Y., Koike H., Sugiyama Y., Motoyoshi K., Wada T., Hishinuma S.,
RA   Mita M., Morita T.;
RT   "Cloning and characterization of novel snake venom proteins that block
RT   smooth muscle contraction.";
RL   Eur. J. Biochem. 269:2708-2715(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=16292255; DOI=10.1038/nature04328;
RA   Fry B.G., Vidal N., Norman J.A., Vonk F.J., Scheib H., Ramjan S.F.R.,
RA   Kuruppu S., Fung K., Blair Hedges S., Richardson M.K., Hodgson W.C.,
RA   Ignjatovic V., Summerhayes R., Kochva E.;
RT   "Early evolution of the venom system in lizards and snakes.";
RL   Nature 439:584-588(2006).
CC   -!- FUNCTION: This protein does not inhibit smooth muscle contraction
CC       elicited by high potassium levels or caffeine.
CC       {ECO:0000269|PubMed:12047379}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   EMBL; AY093955; AAM19739.1; -; mRNA.
DR   EMBL; DQ139899; AAZ75605.1; -; mRNA.
DR   AlphaFoldDB; Q8JGT9; -.
DR   SMR; Q8JGT9; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   CDD; cd05383; CAP_CRISP; 1.
DR   Gene3D; 1.10.10.740; -; 1.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR042076; Crisp-like_dom.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR013871; Cysteine_rich_secretory.
DR   InterPro; IPR034117; SCP_CRISP.
DR   InterPro; IPR003582; ShKT_dom.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   Pfam; PF08562; Crisp; 1.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
DR   PROSITE; PS51670; SHKT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|PubMed:12047379"
FT   CHAIN           19..237
FT                   /note="Cysteine-rich venom protein tigrin"
FT                   /id="PRO_0000006286"
FT   DOMAIN          37..165
FT                   /note="SCP"
FT   DOMAIN          201..232
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        74..152
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        91..166
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        147..163
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        185..192
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        188..197
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        201..232
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        210..226
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        217..230
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   CONFLICT        106
FT                   /note="S -> G (in Ref. 2; AAZ75605)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   237 AA;  26697 MW;  F02A8FAFA7DB8EE0 CRC64;
     MIVFILLSLA AVLRQSFGNV DFNSESPRNP GKQQEIVNIH NSFRRSVRPT ARNMLKMEWY
     SEAASNAERW AYQCAYDHSP RSSRILNGIQ CGENIFMASD PWAWTSIIQD WYDEYRNFVY
     GVGANPPDSV TGHYTQIVWY KSYLVGCAAA YCPSSLYNYF YVCQYCPAGN IQGSTSTPYA
     SGPTCADCPS NCDNGLCTNP CTHKDDYNNC NSLVSDCQSD WDKSHCPATC FCKNKII