ID   CRVP_SISCA              Reviewed;         239 AA.
AC   B0VXV6;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Cysteine-rich venom protein 2;
DE            Short=CRVP;
DE   AltName: Full=Cysteine-rich secretory protein 2;
DE            Short=CRISP-2;
DE   Flags: Precursor;
OS   Sistrurus catenatus edwardsii (Desert massasauga) (Crotalophorus
OS   edwardsii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Crotalinae; Sistrurus.
OX   NCBI_TaxID=8762;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=18096037; DOI=10.1186/1471-2199-8-115;
RA   Pahari S., Mackessy S.P., Kini R.M.;
RT   "The venom gland transcriptome of the Desert Massasauga rattlesnake
RT   (Sistrurus catenatus edwardsii): towards an understanding of venom
RT   composition among advanced snakes (Superfamily Colubroidea).";
RL   BMC Mol. Biol. 8:115-115(2007).
CC   -!- FUNCTION: Weakly blocks contraction of smooth muscle elicited by high
CC       potassium-induced depolarization, but does not block caffeine-
CC       stimulated contraction. May target voltage-gated calcium channels on
CC       smooth muscle (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   EMBL; DQ464263; ABG26992.1; -; mRNA.
DR   AlphaFoldDB; B0VXV6; -.
DR   SMR; B0VXV6; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd05383; CAP_CRISP; 1.
DR   Gene3D; 1.10.10.740; -; 1.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR042076; Crisp-like_dom.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR013871; Cysteine_rich_secretory.
DR   InterPro; IPR034117; SCP_CRISP.
DR   InterPro; IPR003582; ShKT_dom.
DR   InterPro; IPR002413; V5_allergen-like.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   Pfam; PF08562; Crisp; 1.
DR   PRINTS; PR00838; V5ALLERGEN.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
DR   PROSITE; PS51670; SHKT; 1.
PE   2: Evidence at transcript level;
KW   Calcium channel impairing toxin; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000250"
FT   CHAIN           20..239
FT                   /note="Cysteine-rich venom protein 2"
FT                   /id="PRO_0000380663"
FT   DOMAIN          38..166
FT                   /note="SCP"
FT   DOMAIN          198..234
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        75..153
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        92..167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        148..164
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        186..193
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        189..198
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        202..234
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        219..232
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
SQ   SEQUENCE   239 AA;  26542 MW;  91953EBD71514AD2 CRC64;
     MIALIVLPIL AAVLQQSSGS VDFDSESPRK PEIQNKIVDL HNSLRRSVNP TASNMLKMEW
     YSEAAANAER WAYRCIESHS PRDSRVLEGI KCGENIYMSS VPMKWTEIIH IWHGENKNFK
     YGIGADPPNA VTGHYTQIVW YKSYRAGCAA AYCPSLEYSY FYVCQYCPAG NIRGKTATPY
     KSGPPCGDCP SACDNGLCTN PCPKKISTQL PRFGPQAGCQ DKQMQSDCSA TCFCQNKII