ID   CRVP_VIPBN              Reviewed;         221 AA.
AC   B7FDI0;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Cysteine-rich venom protein;
DE            Short=CRVP;
DE   Flags: Precursor; Fragment;
OS   Vipera berus nikolskii (Nikolsky's adder) (Vipera nikolskii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Viperidae; Viperinae; Vipera.
OX   NCBI_TaxID=1808362;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=19041663; DOI=10.1016/j.toxicon.2008.11.001;
RA   Ramazanova A.S., Starkov V.G., Osipov A.V., Ziganshin R.H., Filkin S.Y.,
RA   Tsetlin V.I., Utkin Y.N.;
RT   "Cysteine-rich venom proteins from the snakes of Viperinae subfamily
RT   - molecular cloning and phylogenetic relationship.";
RL   Toxicon 53:162-168(2009).
CC   -!- FUNCTION: Blocks contraction of smooth muscle elicited by high
CC       potassium-induced depolarization, but does not block caffeine-
CC       stimulated contraction. May target voltage-gated calcium channels in
CC       smooth muscle (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   EMBL; AM937249; CAP74088.1; -; mRNA.
DR   AlphaFoldDB; B7FDI0; -.
DR   SMR; B7FDI0; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd05383; CAP_CRISP; 1.
DR   Gene3D; 1.10.10.740; -; 1.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR042076; Crisp-like_dom.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR013871; Cysteine_rich_secretory.
DR   InterPro; IPR034117; SCP_CRISP.
DR   InterPro; IPR003582; ShKT_dom.
DR   InterPro; IPR002413; V5_allergen-like.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   Pfam; PF08562; Crisp; 1.
DR   PRINTS; PR00838; V5ALLERGEN.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
DR   PROSITE; PS51670; SHKT; 1.
PE   2: Evidence at transcript level;
KW   Calcium channel impairing toxin; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          <1..1
FT                   /evidence="ECO:0000255"
FT   CHAIN           2..221
FT                   /note="Cysteine-rich venom protein"
FT                   /id="PRO_5000417202"
FT   DOMAIN          21..148
FT                   /note="SCP"
FT   DOMAIN          184..216
FT                   /note="ShKT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        57..135
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        74..149
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        130..146
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        168..175
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        171..180
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        184..216
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        193..210
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   DISULFID        201..214
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01005"
FT   NON_TER         1
SQ   SEQUENCE   221 AA;  24612 MW;  E2D35825EFE4A96E CRC64;
     GNVDFDSESP RKPEIQNEII DLHNSLRRSV NPTASNMLKM EWYPEAAANA ERWAFRCILS
     HSPRDSRVIG GIKCGENIYM STSPMKWTAI IHKWHGEEKD FVYGQGASPA NAVVGHYTQI
     VWYKSYRSGC AAAYCPSSEY KYFYVCQYCP AGNMQGKTAT PYTSGPPCGD CPSACDNGLC
     TNPCTHEDKF TNCKDLVKQG CNNNYLKTNC PASCSCHNEI I