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CRWN3_ARATH
ID   CRWN3_ARATH             Reviewed;        1085 AA.
AC   Q9CA42;
DT   29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Protein CROWDED NUCLEI 3 {ECO:0000303|PubMed:24308514};
DE   AltName: Full=Protein LITTLE NUCLEI 3 {ECO:0000303|PubMed:17873096};
GN   Name=CRWN3 {ECO:0000303|PubMed:24308514};
GN   Synonyms=LINC3 {ECO:0000303|PubMed:17873096};
GN   OrderedLocusNames=At1g68790 {ECO:0000312|Araport:AT1G68790};
GN   ORFNames=F14K14.10 {ECO:0000312|EMBL:AAG52034.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1059.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-318 AND LYS-661, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=cv. Landsberg erecta;
RX   PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA   Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT   "Multidimensional protein identification technology (MudPIT) analysis of
RT   ubiquitinated proteins in plants.";
RL   Mol. Cell. Proteomics 6:601-610(2007).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=17873096; DOI=10.1105/tpc.107.053231;
RA   Dittmer T.A., Stacey N.J., Sugimoto-Shirasu K., Richards E.J.;
RT   "LITTLE NUCLEI genes affecting nuclear morphology in Arabidopsis
RT   thaliana.";
RL   Plant Cell 19:2793-2803(2007).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-843 AND SER-910, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=cv. Columbia;
RX   PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA   Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA   Rathjen J.P., Peck S.C.;
RT   "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT   thaliana.";
RL   J. Proteomics 72:439-451(2009).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-910, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
RN   [8]
RP   FUNCTION, DISRUPTION PHENOTYPE, GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=24308514; DOI=10.1186/1471-2229-13-200;
RA   Wang H., Dittmer T.A., Richards E.J.;
RT   "Arabidopsis CROWDED NUCLEI (CRWN) proteins are required for nuclear size
RT   control and heterochromatin organization.";
RL   BMC Plant Biol. 13:200-200(2013).
RN   [9]
RP   FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=23396599; DOI=10.1093/pcp/pct031;
RA   Sakamoto Y., Takagi S.;
RT   "LITTLE NUCLEI 1 and 4 regulate nuclear morphology in Arabidopsis
RT   thaliana.";
RL   Plant Cell Physiol. 54:622-633(2013).
CC   -!- FUNCTION: Component of SUN-protein-containing multivariate complexes
CC       also called LINC complexes which link the nucleoskeleton and
CC       cytoskeleton by providing versatile outer nuclear membrane attachment
CC       sites for cytoskeletal filaments (By similarity). Required for nucleus
CC       structure organization (e.g. size and shape) (PubMed:24308514,
CC       PubMed:23396599). {ECO:0000250|UniProtKB:Q6ZWR6,
CC       ECO:0000269|PubMed:23396599, ECO:0000269|PubMed:24308514}.
CC   -!- SUBUNIT: Core component of the LINC complex which is composed of inner
CC       nuclear membrane SUN domain-containing proteins coupled to outer
CC       nuclear membrane WIP proteins, the nucleoskeletal CRWN/LINC proteins,
CC       and, possibly, KAKU4. {ECO:0000250|UniProtKB:F4HRT5}.
CC   -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000250|UniProtKB:F4HRT5};
CC       Peripheral membrane protein {ECO:0000250|UniProtKB:F4HRT5}. Nucleus,
CC       nucleoplasm {ECO:0000269|PubMed:23396599}. Cytoplasm
CC       {ECO:0000269|PubMed:23396599}. Nucleus lamina
CC       {ECO:0000250|UniProtKB:F4HRT5}. Note=Recruited to the nucleus envelope
CC       (NE) by SUN proteins and is immobilised therein (By similarity).
CC       Punctate or bundle-like structures are detected, especially in
CC       trichomes where bundle-shape localization pattern along the long axis
CC       of the nucleus is observed. During prometaphase to anaphase, localized
CC       diffusely in the cytoplasm. Later transferred from the cytoplasm to the
CC       chromatin surface, preferentially assembling to the distal surface of
CC       the chromatin. Relocalized to the nuclear periphery during late
CC       telophase (PubMed:23396599). {ECO:0000250|UniProtKB:F4HRT5,
CC       ECO:0000269|PubMed:23396599}.
CC   -!- TISSUE SPECIFICITY: Expressed at low levels in roots, leaves, flowers
CC       and flower stalks. {ECO:0000269|PubMed:23396599}.
CC   -!- DISRUPTION PHENOTYPE: Altered nuclear morphology. Plants lacking both
CC       CRWN1 and CRWN3 exhibit markedly smaller rosettes. Plants lacking
CC       CRWN1, CRWN3 and CRWN4 are extremely stunted and set few seed.
CC       {ECO:0000269|PubMed:23396599, ECO:0000269|PubMed:24308514}.
CC   -!- SIMILARITY: Belongs to the CRWN family. {ECO:0000305}.
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DR   EMBL; AC011914; AAG52034.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE34839.1; -; Genomic_DNA.
DR   EMBL; AY072196; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; F96712; F96712.
DR   RefSeq; NP_177046.1; NM_105552.3.
DR   AlphaFoldDB; Q9CA42; -.
DR   SMR; Q9CA42; -.
DR   IntAct; Q9CA42; 2.
DR   STRING; 3702.AT1G68790.1; -.
DR   iPTMnet; Q9CA42; -.
DR   PaxDb; Q9CA42; -.
DR   PRIDE; Q9CA42; -.
DR   ProteomicsDB; 224502; -.
DR   EnsemblPlants; AT1G68790.1; AT1G68790.1; AT1G68790.
DR   GeneID; 843210; -.
DR   Gramene; AT1G68790.1; AT1G68790.1; AT1G68790.
DR   KEGG; ath:AT1G68790; -.
DR   Araport; AT1G68790; -.
DR   TAIR; locus:2012423; AT1G68790.
DR   eggNOG; ENOG502QUGA; Eukaryota.
DR   HOGENOM; CLU_004241_0_0_1; -.
DR   InParanoid; Q9CA42; -.
DR   OMA; EKKEWTA; -.
DR   OrthoDB; 162808at2759; -.
DR   PhylomeDB; Q9CA42; -.
DR   PRO; PR:Q9CA42; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9CA42; baseline and differential.
DR   Genevisible; Q9CA42; AT.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005652; C:nuclear lamina; IEA:UniProtKB-SubCell.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR   GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR   GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR   GO; GO:0006997; P:nucleus organization; IMP:UniProtKB.
DR   InterPro; IPR040418; CRWN.
DR   PANTHER; PTHR31908; PTHR31908; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Isopeptide bond; Membrane; Nucleus; Phosphoprotein;
KW   Reference proteome; Ubl conjugation.
FT   CHAIN           1..1085
FT                   /note="Protein CROWDED NUCLEI 3"
FT                   /id="PRO_0000432821"
FT   REGION          801..997
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1020..1077
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          51..149
FT                   /evidence="ECO:0000255"
FT   COILED          185..695
FT                   /evidence="ECO:0000255"
FT   MOTIF           404..411
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT   COMPBIAS        809..828
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        829..844
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        880..899
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        945..997
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1049..1068
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         764
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:F4HRT5"
FT   MOD_RES         787
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:F4HRT5"
FT   MOD_RES         825
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:F4HRT5"
FT   MOD_RES         843
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19245862"
FT   MOD_RES         910
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19245862,
FT                   ECO:0007744|PubMed:19376835"
FT   CROSSLNK        318
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0007744|PubMed:17272265"
FT   CROSSLNK        661
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0007744|PubMed:17272265"
SQ   SEQUENCE   1085 AA;  127204 MW;  ED0F40CA45BE355B CRC64;
     MFTPQRNRWP ETDRKGKAIA FSDEIITPPP QRVLLREDDD WQKFKEVGLL DEASLERKDR
     DALIEKILKL EKELFDYQHN MGLLLIEKKQ WTSTNNELQQ AYDEAMEMLK REKTSNAITL
     NEADKREENL RKALIDEKQF VAELENDLKY WQREHSVVKS TSEAKLEEAN ALVIGMKEKA
     LEVDRERAIA EEKFSVMNRK SSELERKLKE VETREKVHQR EHLSLVTERE AHEAVFYKQR
     EDLQEWEKKL TLEEDRLSEV KRSINHREER VMENERTIEK KEKILENLQQ KISVAKSELT
     EKEESIKIKL NDISLKEKDF EAMKAKVDIK EKELHEFEEN LIEREQMEIG KLLDDQKAVL
     DSRRREFEME LEQMRRSLDE ELEGKKAEIE QLQVEISHKE EKLAKREAAL EKKEEGVKKK
     EKDLDARLKT VKEKEKALKA EEKKLHMENE RLLEDKECLR KLKDEIEEIG TETTKQESRI
     REEHESLRIT KEERVEFLRL QSELKQQIDK VKQEEELLLK EREELKQDKE RFEKEWEALD
     KKRANITREQ NEVAEENEKL RNLQISEKHR LKREEMTSRD NLKRELDGVK MQKESFEADM
     EDLEMQKRNL DMEFQRQEEA GERDFNERAR TYEKRSQEEL DNINYTKKLA QREMEEMQYE
     KLALEREREQ ISVRKKLLKE QEAEMHKDIT ELDVLRSSLK EKRKEFICER ERFLVFLEKL
     KSCSSCGEIT ENFVLSDLRL PDVEDGDKRF GKQKLKAEEA LNISPSAENS KRTSLLGKIA
     SKLLSISPIG KTDKVTDLGI TVKLPESSQP DDSLDRVSGE DHEPSATEQS FTDSRIQEGP
     EGSLQSEMKS DKPRRGRGRG RGRGKSVRGR SQATKAVSRD SKPSDGETPR KRQREQTSRI
     TESEQAAGDS DEGVDSITTG GRRKKRQIAV PVSQTPGQTR YQLRRHRNVG TEEDKAQASK
     GATEKQERVN DDIRKVPSPK ETRTPPEGEN RENGKAEVLV ETVTHEEIVT VETETVFKVN
     NTGKNPVEDP QLEVGGSGEI REHGEEDDEN ISMIEEENEG EEEEETERQG NDASIGKKIW
     VFFTT
 
 
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