ID   CRYD_DANRE              Reviewed;         520 AA.
AC   Q4KML2; Q75WS5;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Cryptochrome DASH;
DE   AltName: Full=Protein CRY-DASH;
DE            Short=zCRY-DASH;
GN   Name=cry-dash;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND COFACTOR.
RX   PubMed=15147276; DOI=10.1111/j.1356-9597.2004.00738.x;
RA   Daiyasu H., Ishikawa T., Kuma K., Iwai S., Todo T., Toh H.;
RT   "Identification of cryptochrome DASH from vertebrates.";
RL   Genes Cells 9:479-495(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May have a photoreceptor function (By similarity). Has weak
CC       cyclobutyl pyrimidine photolyase activity when expressed in E.coli and
CC       when tested in vitro. {ECO:0000250, ECO:0000269|PubMed:15147276}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000269|PubMed:15147276};
CC       Note=Binds 1 FAD per subunit. {ECO:0000269|PubMed:15147276};
CC   -!- COFACTOR:
CC       Name=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate;
CC         Xref=ChEBI:CHEBI:15636; Evidence={ECO:0000269|PubMed:15147276};
CC       Note=Binds 1 5,10-methenyltetrahydrofolate (MTHF) non-covalently per
CC       subunit. {ECO:0000269|PubMed:15147276};
CC   -!- SIMILARITY: Belongs to the DNA photolyase class-1 family.
CC       {ECO:0000305}.
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DR   EMBL; AB120759; BAD08600.1; -; mRNA.
DR   EMBL; BC098514; AAH98514.1; -; mRNA.
DR   RefSeq; NP_991249.1; NM_205686.1.
DR   AlphaFoldDB; Q4KML2; -.
DR   SMR; Q4KML2; -.
DR   STRING; 7955.ENSDARP00000121988; -.
DR   PaxDb; Q4KML2; -.
DR   GeneID; 402986; -.
DR   KEGG; dre:402986; -.
DR   CTD; 402986; -.
DR   ZFIN; ZDB-GENE-040617-2; cry-dash.
DR   eggNOG; KOG0133; Eukaryota.
DR   InParanoid; Q4KML2; -.
DR   OrthoDB; 378952at2759; -.
DR   PhylomeDB; Q4KML2; -.
DR   PRO; PR:Q4KML2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0003684; F:damaged DNA binding; IDA:ZFIN.
DR   GO; GO:0003904; F:deoxyribodipyrimidine photo-lyase activity; IDA:ZFIN.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0071949; F:FAD binding; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IDA:ZFIN.
DR   GO; GO:0000719; P:photoreactive repair; IDA:ZFIN.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR014133; Cry_DASH.
DR   InterPro; IPR036134; Crypto/Photolyase_FAD-like_sf.
DR   InterPro; IPR036155; Crypto/Photolyase_N_sf.
DR   InterPro; IPR005101; Cryptochr/Photolyase_FAD-bd.
DR   InterPro; IPR002081; Cryptochrome/DNA_photolyase_1.
DR   InterPro; IPR006050; DNA_photolyase_N.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR11455; PTHR11455; 1.
DR   Pfam; PF00875; DNA_photolyase; 1.
DR   Pfam; PF03441; FAD_binding_7; 1.
DR   PRINTS; PR00147; DNAPHOTLYASE.
DR   SUPFAM; SSF48173; SSF48173; 1.
DR   SUPFAM; SSF52425; SSF52425; 1.
DR   TIGRFAMs; TIGR02765; crypto_DASH; 1.
DR   PROSITE; PS51645; PHR_CRY_ALPHA_BETA; 1.
PE   2: Evidence at transcript level;
KW   Chromophore; FAD; Flavoprotein; Reference proteome.
FT   CHAIN           1..520
FT                   /note="Cryptochrome DASH"
FT                   /id="PRO_0000235316"
FT   DOMAIN          5..141
FT                   /note="Photolyase/cryptochrome alpha/beta"
FT   REGION          479..504
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        479..498
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        38
FT                   /note="C -> S (in Ref. 2; AAH98514)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        93
FT                   /note="C -> F (in Ref. 2; AAH98514)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        324
FT                   /note="N -> K (in Ref. 1; BAD08600)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   520 AA;  59922 MW;  1CFAE97F9C1A7213 CRC64;
     MSASRTVICL LRNDLRLHDN EVFHWAQRNA EHIIPLYCFD PRHYQGTYHY NFPKTGPFRL
     RFLLDSVKDL RALLKKHGST LLVRQGKPED VVCELIKQLG SVSTVAFHEE VASEEKSVEE
     KLKEICCQNK VRVQTFWGST LYHRDDLPFS HIGGLPDVYT QFRKAVEAQG RVRPVLSTPE
     QVKSPPSGLE EGPIPTFDSL GQTEPLDDCR SAFPCRGGET EALARLKHYF WDTNAVATYK
     ETRNGMIGVD FSTKFSPWLA LGCISPRYIY EQIKKYEVER TANQSTYWVI FELLWRDYFK
     FVALKYGNRI FYMNGLQDKH VPWNTDMKMF DAWKEGRTGV PFVDANMREL ALTGFMSNRG
     RQNVASFLTK DLGLDWRLGA EWFEYLLVDH DVCSNYGNWL YSAGIGNDPR ENRKFNMIKQ
     GLDYDNNGDY VRQWVPELRG IKGGDVHTPW TLSNSALSHA QVSLNQTYPC PIITAPEWSR
     HVNNKSSGPS SSKGRKGSSY TARQHKDRGI DFYFSKNKHF