ID   CRYM_BOVIN              Reviewed;         314 AA.
AC   Q2KHX6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Ketimine reductase mu-crystallin;
DE            EC=1.5.1.25;
DE   AltName: Full=NADP-regulated thyroid-hormone-binding protein;
GN   Name=CRYM;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hypothalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Specifically catalyzes the reduction of imine bonds in brain
CC       substrates that may include cystathionine ketimine (CysK) and
CC       lanthionine ketimine (LK). Binds thyroid hormone which is a strong
CC       reversible inhibitor. Presumably involved in the regulation of the free
CC       intracellular concentration of triiodothyronine and access to its
CC       nuclear receptors (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-1,4-thiomorpholine-3-carboxylate + NAD(+) = 3,4-
CC         dehydrothiomorpholine-3-carboxylate + 2 H(+) + NADH;
CC         Xref=Rhea:RHEA:12504, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:58517, ChEBI:CHEBI:176873;
CC         EC=1.5.1.25;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3R)-1,4-thiomorpholine-3-carboxylate + NADP(+) = 3,4-
CC         dehydrothiomorpholine-3-carboxylate + 2 H(+) + NADPH;
CC         Xref=Rhea:RHEA:12500, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:58517, ChEBI:CHEBI:176873;
CC         EC=1.5.1.25;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ornithine cyclodeaminase/mu-crystallin
CC       family. {ECO:0000305}.
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DR   EMBL; BC112846; AAI12847.1; -; mRNA.
DR   RefSeq; NP_001039379.1; NM_001045914.2.
DR   AlphaFoldDB; Q2KHX6; -.
DR   SMR; Q2KHX6; -.
DR   STRING; 9913.ENSBTAP00000012972; -.
DR   PaxDb; Q2KHX6; -.
DR   Ensembl; ENSBTAT00000012972; ENSBTAP00000012972; ENSBTAG00000009842.
DR   GeneID; 505167; -.
DR   KEGG; bta:505167; -.
DR   CTD; 1428; -.
DR   VEuPathDB; HostDB:ENSBTAG00000009842; -.
DR   VGNC; VGNC:27745; CRYM.
DR   eggNOG; KOG3007; Eukaryota.
DR   GeneTree; ENSGT00390000000237; -.
DR   HOGENOM; CLU_042088_1_1_1; -.
DR   InParanoid; Q2KHX6; -.
DR   OMA; AVKAFTY; -.
DR   OrthoDB; 1345495at2759; -.
DR   TreeFam; TF105309; -.
DR   Reactome; R-BTA-71064; Lysine catabolism.
DR   Proteomes; UP000009136; Chromosome 25.
DR   Bgee; ENSBTAG00000009842; Expressed in Ammon's horn and 91 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR   GO; GO:0005739; C:mitochondrion; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR   GO; GO:0042562; F:hormone binding; IBA:GO_Central.
DR   GO; GO:0050661; F:NADP binding; IEA:Ensembl.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:Ensembl.
DR   GO; GO:0047127; F:thiomorpholine-carboxylate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0070324; F:thyroid hormone binding; IBA:GO_Central.
DR   GO; GO:0003714; F:transcription corepressor activity; IEA:Ensembl.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   GO; GO:0007605; P:sensory perception of sound; IEA:Ensembl.
DR   GO; GO:0042403; P:thyroid hormone metabolic process; IBA:GO_Central.
DR   GO; GO:0070327; P:thyroid hormone transport; IEA:Ensembl.
DR   Gene3D; 3.30.1780.10; -; 1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR003462; ODC_Mu_crystall.
DR   InterPro; IPR023401; ODC_N.
DR   PANTHER; PTHR13812; PTHR13812; 1.
DR   Pfam; PF02423; OCD_Mu_crystall; 1.
DR   PIRSF; PIRSF001439; CryM; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; NAD; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..313
FT                   /note="Ketimine reductase mu-crystallin"
FT                   /id="PRO_0000328261"
FT   BINDING         143..148
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         168
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         169
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   314 AA;  33747 MW;  D9EAE1F47B7CAE2B CRC64;
     MSSRPVFLSA ADVQDHLRSS SLLIAPLETA LANFSSGPDG GVVQPVRTVV PVAKHRGFLG
     VMPAYSAAED ALTTKLVTFY EDHSATSTVP SHQATVLLFQ PSNGSLLAVM DGNVITAKRT
     AAVSAIATKF LKPPNSEVLC ILGAGVQAYS HYEVFTEQFF FKEVRIWNRT KENAEKFVNT
     VPGEVRICSS VQEAVTGADV IITVTMATEP ILFGEWVKPG AHINAIGASR PDWRELDDEL
     MKQAVLYVDS QEAALKESGD VLLSGAEIFA ELGEVVKGVK PAHCEKTTVF KSLGMAVEDM
     VAAKLVYDSW SSGK