ID   CRYS_DICDI              Reviewed;         550 AA.
AC   P21837; Q54N20;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Crystal protein;
DE   Flags: Precursor;
GN   Name=cryS; ORFNames=DDB_G0285419;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF N-TERMINUS.
RC   STRAIN=AX2, and AX3;
RX   PubMed=2307702; DOI=10.1083/jcb.110.3.669;
RA   Bomblies L., Biegelmann E., Doering V., Gerisch G., Krafft-Czepa H.,
RA   Noegel A.A., Schleicher M., Humbel B.M.;
RT   "Membrane-enclosed crystals in Dictyostelium discoideum cells, consisting
RT   of developmentally regulated proteins with sequence similarities to known
RT   esterases.";
RL   J. Cell Biol. 110:669-679(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, esterosome membrane.
CC       Note=Membrane-enclosed crystals (esterosome).
CC   -!- DEVELOPMENTAL STAGE: Crystals accumulates between growth and
CC       aggregation stage and disappear only after the spore germination.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; X52464; CAA36702.1; -; mRNA.
DR   EMBL; AAFI02000079; EAL64547.1; -; Genomic_DNA.
DR   PIR; A34576; A34576.
DR   RefSeq; XP_638123.1; XM_633031.1.
DR   AlphaFoldDB; P21837; -.
DR   SMR; P21837; -.
DR   ESTHER; dicdi-crysp; Cholinesterase-like.
DR   PaxDb; P21837; -.
DR   ABCD; P21837; 1 sequenced antibody.
DR   EnsemblProtists; EAL64547; EAL64547; DDB_G0285419.
DR   GeneID; 8625172; -.
DR   KEGG; ddi:DDB_G0285419; -.
DR   dictyBase; DDB_G0285419; cryS.
DR   eggNOG; KOG1516; Eukaryota.
DR   HOGENOM; CLU_006586_13_0_1; -.
DR   InParanoid; P21837; -.
DR   OMA; PLEMFIP; -.
DR   PhylomeDB; P21837; -.
DR   Reactome; R-DDI-112311; Neurotransmitter clearance.
DR   Reactome; R-DDI-1483191; Synthesis of PC.
DR   Reactome; R-DDI-211945; Phase I - Functionalization of compounds.
DR   Reactome; R-DDI-8964038; LDL clearance.
DR   Reactome; R-DDI-9749641; Aspirin ADME.
DR   PRO; PR:P21837; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0032009; C:early phagosome; IMP:dictyBase.
DR   GO; GO:0033118; C:esterosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031012; C:extracellular matrix; HDA:dictyBase.
DR   GO; GO:0005764; C:lysosome; IMP:dictyBase.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IDA:dictyBase.
DR   GO; GO:0006897; P:endocytosis; IMP:dictyBase.
DR   GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR   GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR019819; Carboxylesterase_B_CS.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR   PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasmic vesicle; Direct protein sequencing; Disulfide bond;
KW   Glycoprotein; Hydrolase; Membrane; Reference proteome; Serine esterase;
KW   Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:2307702"
FT   CHAIN           20..550
FT                   /note="Crystal protein"
FT                   /id="PRO_0000008618"
FT   ACT_SITE        215
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   ACT_SITE        340
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        443
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        156
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        506
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        91..111
FT                   /evidence="ECO:0000250"
FT   DISULFID        267..274
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   550 AA;  61066 MW;  3EE77153B3341000 CRC64;
     MNKIIILLII LLSFDIISAA KKFGRKGIRT LGDNEVLLSD GAIRGTVTDT HRVFYGIPFA
     RPPIDELRYE DPQPPKPWSY VRDGTKQRDQ CIQDCKLGKG SCSEVGTSED CLYLDVFIPR
     TVNPGSKVPV MVFIPGGAFT QGTGSCPLYD GLKFANSSVI VVNVNYRLGV LGFLCTGLLS
     GNFGFLDQVM ALDWVQENIE VFGGDKNQVT IYGESAGAFS VAAHLSSEKS EGKFHRAILS
     STPYTVGLKS QTVARGFAGR FSSKIGCDLE DIDCHRSKSP EEILAIQKEL GLAIGDKILD
     AFTIWSPVVD GINVNEQPLT MIKQGTTHDV PTIIGDNQDE AILFVYMTYK NVVIPSSYRT
     MVHVLFGIAN GNKVLEHYPL PGFLKDSRPI LSKLLTDYLF RCPGRYHVSK SAQANESPIY
     HYQYKQVLSG GHSFEACEGL VCHGTELPMV FNTYESALDL DLEEEEEEFA EQLNNYFVNF
     IKYSNPSHPN GLPTPKVWNP TTKTTNTSLV MKLGFEVKDL ITNDPKCDLF DSLSYNGYTK
     DQNRMRKSKK