2SS_SOYBN
ID 2SS_SOYBN Reviewed; 158 AA.
AC P19594; Q53WV6;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=2S seed storage albumin protein {ECO:0000305};
DE AltName: Full=2S albumin {ECO:0000303|Ref.2};
DE AltName: Full=GM2S-1 {ECO:0000303|Ref.2};
DE AltName: Full=Napin-type 2S albumin 3 {ECO:0000303|Ref.1};
DE Contains:
DE RecName: Full=2S albumin small chain {ECO:0000303|Ref.2};
DE AltName: Full=Aspartic acid-rich peptide;
DE AltName: Full=Lunasin {ECO:0000303|PubMed:10331812};
DE Contains:
DE RecName: Full=2S albumin large chain {ECO:0000303|Ref.2};
DE AltName: Full=8 kDa methionine-rich protein;
DE Short=8 kDa MRP;
DE Flags: Precursor;
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Pioneer 9341; TISSUE=Seed;
RA Jung R., Hastings C., Coughlan S.J., Hu W.-N.;
RT "sulfur- and lysine-rich napin-type 2S albumins from soybean seed.";
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Hodgson 78; TISSUE=Cotyledon;
RA Galvez A.F., Revilleza M.J.R., de Lumen B.O.;
RT "A novel methionine-rich protein from soybean cotyledon: cloning and
RT characterization of cDNA.";
RL (er) Plant Gene Register PGR97-103(1997).
RN [3]
RP PROTEIN SEQUENCE OF 22-64.
RC TISSUE=Seed;
RX PubMed=3611081; DOI=10.1016/s0021-9258(18)60989-5;
RA Odani S., Koide T., Ono T.;
RT "Amino acid sequence of a soybean (Glycine max) seed polypeptide having a
RT poly(L-aspartic acid) structure.";
RL J. Biol. Chem. 262:10502-10505(1987).
RN [4]
RP PROTEIN SEQUENCE OF 82-96.
RX AGRICOLA=IND20620639; DOI=10.1021/jf960063u;
RA Revilleza M.J., Galvez A.F., Krenz D.C., de Lumen B.O.;
RT "An 8 kDa methionine-rich protein (MRP) from soybean (Glycine max)
RT cotyledon: identification, purification and N-terminal sequence.";
RL J. Agric. Food Chem. 44:2930-2935(1996).
RN [5]
RP FUNCTION (2S ALBUMIN SMALL CHAIN), AND DOMAIN (2S ALBUMIN SMALL CHAIN).
RX PubMed=10331812; DOI=10.1038/8676;
RA Galvez A.F., de Lumen B.O.;
RT "A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of
RT mammalian cells.";
RL Nat. Biotechnol. 17:495-500(1999).
CC -!- FUNCTION: This is a 2S seed storage protein. {ECO:0000305|Ref.1}.
CC -!- FUNCTION: [2S albumin small chain]: Binds to mammalian chromatin,
CC preventing the normal formation of the kinetochore complex in the
CC centromere and leading to the disruption of mitosis (PubMed:10331812).
CC {ECO:0000269|PubMed:10331812}.
CC -!- SUBUNIT: The protein consists of two chains linked by 2 disulfide
CC bonds.
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons (Ref.2). Maximal expression
CC in parenchyma cells undergoing DNA endoreduplication and cell expansion
CC but not in actively dividing cells of the cotyledon (PubMed:10331812).
CC {ECO:0000269|Ref.2, ECO:0000303|PubMed:10331812}.
CC -!- DEVELOPMENTAL STAGE: Expressed from 3 weeks after flowering until the
CC early desiccation stage, 7 weeks after flowering. Not detected in
CC mature seeds. This temporal expression coincides with the initiation of
CC mitotic arrest and DNA endoreduplication in developing soybean
CC cotyledon. {ECO:0000269|Ref.2}.
CC -!- DOMAIN: [2S albumin small chain]: The poly-Asp C-terminal domain (56-
CC 64) of the small chain is necessary for its histone binding and
CC antimitotic effect. {ECO:0000269|PubMed:10331812}.
CC -!- MISCELLANEOUS: [2S albumin small chain]: The antimitotic effect leads
CC to a cytotoxic response in mammalian cells.
CC {ECO:0000269|PubMed:10331812}.
CC -!- SIMILARITY: Belongs to the 2S seed storage albumins family.
CC {ECO:0000305}.
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DR EMBL; U71195; AAD00178.1; -; mRNA.
DR EMBL; AF005030; AAB71140.1; -; mRNA.
DR PIR; T05710; T05710.
DR RefSeq; NP_001238443.1; NM_001251514.1.
DR AlphaFoldDB; P19594; -.
DR SMR; P19594; -.
DR STRING; 3847.GLYMA13G36400.1; -.
DR Allergome; 11407; Gly m 8.0101.
DR Allergome; 1189; Gly m 8.
DR PRIDE; P19594; -.
DR EnsemblPlants; KRH22246; KRH22246; GLYMA_13G288100.
DR GeneID; 548076; -.
DR Gramene; KRH22246; KRH22246; GLYMA_13G288100.
DR KEGG; gmx:548076; -.
DR OMA; PCEKHIM; -.
DR OrthoDB; 1646659at2759; -.
DR Proteomes; UP000008827; Chromosome 13.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR DisProt; DP01154; -.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000617; Napin/2SS/CON.
DR PANTHER; PTHR35496; PTHR35496; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Reference proteome;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:3611081"
FT CHAIN 22..64
FT /note="2S albumin small chain"
FT /id="PRO_0000032158"
FT PROPEP 65..81
FT /evidence="ECO:0000269|Ref.4"
FT /id="PRO_0000032159"
FT CHAIN 82..158
FT /note="2S albumin large chain"
FT /id="PRO_0000032160"
FT MOTIF 54..56
FT /note="Cell attachment site"
FT /evidence="ECO:0000255"
SQ SEQUENCE 158 AA; 18460 MW; C1F42723B0F62D81 CRC64;
MTKFTILLIS LLFCIAHTCS ASKWQHQQDS CRKQLQGVNL TPCEKHIMEK IQGRGDDDDD
DDDDNHILRT MRGRINYIRR NEGKDEDEEE EGHMQKCCTE MSELRSPKCQ CKALQKIMEN
QSEELEEKQK KKMEKELINL ATMCRFGPMI QCDLSSDD
