ID   CS13A_PALPW             Reviewed;        1154 AA.
AC   E4T0I2;
DT   22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas13a {ECO:0000303|PubMed:28475872};
DE            Short=EndoRNase;
DE            EC=3.1.-.-;
DE   AltName: Full=PprCas13a {ECO:0000303|PubMed:28475872};
GN   Name=cas13a {ECO:0000303|PubMed:28475872}; OrderedLocusNames=Palpr_0179;
OS   Paludibacter propionicigenes (strain DSM 17365 / JCM 13257 / WB4).
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Paludibacteraceae;
OC   Paludibacter.
OX   NCBI_TaxID=694427;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17365 / JCM 13257 / WB4;
RX   PubMed=21475585; DOI=10.4056/sigs.1503846;
RA   Gronow S., Munk C., Lapidus A., Nolan M., Lucas S., Hammon N.,
RA   Deshpande S., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S.,
RA   Liolios K., Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA   Brambilla E., Rohde M., Goker M., Detter J.C., Woyke T., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Paludibacter propionicigenes type strain
RT   (WB4).";
RL   Stand. Genomic Sci. 4:36-44(2011).
RN   [2]
RP   FUNCTION IN CRRNA PROCESSING, FUNCTION IN TARGET SSRNA CLEAVAGE, FUNCTION
RP   AS AN ENDORIBONUCLEASE, AND ACTIVITY REGULATION.
RX   PubMed=28475872; DOI=10.1016/j.molcel.2017.04.008;
RA   East-Seletsky A., O'Connell M.R., Burstein D., Knott G.J., Doudna J.A.;
RT   "RNA targeting by functionally orthogonal type VI-A CRISPR-Cas enzymes.";
RL   Mol. Cell 66:373-383(2017).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. Unlike
CC       many single-component effectors, this CRISPR-Cas system targets RNA
CC       (PubMed:28475872). CRISPR clusters are transcribed from pre-CRISPR RNA
CC       (crRNA) and processed into crRNA by this protein (PubMed:28475872).
CC       Cleaves linear target ssRNA in a pre-crRNA-dependent fashion,
CC       preferentially before U residues (PubMed:28475872). Binding a viable
CC       target RNA target activates this protein for non-specific RNA
CC       degradation in vitro (called collateral RNA degradation), which is
CC       fairly sensitive as it requires picomolar levels of viable target RNA
CC       (PubMed:28475872). {ECO:0000269|PubMed:28475872}.
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250|UniProtKB:C7NBY4};
CC       Note=Pre-crRNA processing is metal independent, while crRNA-guided
CC       target RNA cleavage is dependent on divalent metal.
CC       {ECO:0000250|UniProtKB:C7NBY4};
CC   -!- ACTIVITY REGULATION: Target RNA acts as an activator for non-specific
CC       ssRNA degradation (PubMed:28475872). {ECO:0000269|PubMed:28475872}.
CC   -!- DOMAIN: The target ssRNase active sites are probably within the 2 HEPN-
CC       like folds, and the 2 folds interact in vivo.
CC       {ECO:0000250|UniProtKB:C7NBY4}.
CC   -!- MISCELLANEOUS: Part of a type VI-A uridine-prefering CRISPR-Cas system.
CC       {ECO:0000305|PubMed:28475872}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas13a
CC       family. {ECO:0000305}.
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DR   EMBL; CP002345; ADQ78341.1; -; Genomic_DNA.
DR   RefSeq; WP_013443710.1; NC_014734.1.
DR   AlphaFoldDB; E4T0I2; -.
DR   SMR; E4T0I2; -.
DR   EnsemblBacteria; ADQ78341; ADQ78341; Palpr_0179.
DR   KEGG; ppn:Palpr_0179; -.
DR   eggNOG; ENOG5032JQS; Bacteria.
DR   HOGENOM; CLU_280435_0_0_10; -.
DR   OMA; EETRINF; -.
DR   OrthoDB; 1059045at2; -.
DR   Proteomes; UP000008718; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   Repeat; RNA-binding.
FT   CHAIN           1..1154
FT                   /note="CRISPR-associated endoribonuclease Cas13a"
FT                   /id="PRO_0000442271"
FT   REGION          330..466
FT                   /note="HEPN-like fold 1"
FT                   /evidence="ECO:0000250|UniProtKB:C7NBY4"
FT   REGION          923..1154
FT                   /note="HEPN-like fold 2"
FT                   /evidence="ECO:0000250|UniProtKB:C7NBY4"
SQ   SEQUENCE   1154 AA;  134252 MW;  94330DB7DE13E52D CRC64;
     MRVSKVKVKD GGKDKMVLVH RKTTGAQLVY SGQPVSNETS NILPEKKRQS FDLSTLNKTI
     IKFDTAKKQK LNVDQYKIVE KIFKYPKQEL PKQIKAEEIL PFLNHKFQEP VKYWKNGKEE
     SFNLTLLIVE AVQAQDKRKL QPYYDWKTWY IQTKSDLLKK SIENNRIDLT ENLSKRKKAL
     LAWETEFTAS GSIDLTHYHK VYMTDVLCKM LQDVKPLTDD KGKINTNAYH RGLKKALQNH
     QPAIFGTREV PNEANRADNQ LSIYHLEVVK YLEHYFPIKT SKRRNTADDI AHYLKAQTLK
     TTIEKQLVNA IRANIIQQGK TNHHELKADT TSNDLIRIKT NEAFVLNLTG TCAFAANNIR
     NMVDNEQTND ILGKGDFIKS LLKDNTNSQL YSFFFGEGLS TNKAEKETQL WGIRGAVQQI
     RNNVNHYKKD ALKTVFNISN FENPTITDPK QQTNYADTIY KARFINELEK IPEAFAQQLK
     TGGAVSYYTI ENLKSLLTTF QFSLCRSTIP FAPGFKKVFN GGINYQNAKQ DESFYELMLE
     QYLRKENFAE ESYNARYFML KLIYNNLFLP GFTTDRKAFA DSVGFVQMQN KKQAEKVNPR
     KKEAYAFEAV RPMTAADSIA DYMAYVQSEL MQEQNKKEEK VAEETRINFE KFVLQVFIKG
     FDSFLRAKEF DFVQMPQPQL TATASNQQKA DKLNQLEASI TADCKLTPQY AKADDATHIA
     FYVFCKLLDA AHLSNLRNEL IKFRESVNEF KFHHLLEIIE ICLLSADVVP TDYRDLYSSE
     ADCLARLRPF IEQGADITNW SDLFVQSDKH SPVIHANIEL SVKYGTTKLL EQIINKDTQF
     KTTEANFTAW NTAQKSIEQL IKQREDHHEQ WVKAKNADDK EKQERKREKS NFAQKFIEKH
     GDDYLDICDY INTYNWLDNK MHFVHLNRLH GLTIELLGRM AGFVALFDRD FQFFDEQQIA
     DEFKLHGFVN LHSIDKKLNE VPTKKIKEIY DIRNKIIQIN GNKINESVRA NLIQFISSKR
     NYYNNAFLHV SNDEIKEKQM YDIRNHIAHF NYLTKDAADF SLIDLINELR ELLHYDRKLK
     NAVSKAFIDL FDKHGMILKL KLNADHKLKV ESLEPKKIYH LGSSAKDKPE YQYCTNQVMM
     AYCNMCRSLL EMKK