ID   CS32_ECOLX              Reviewed;         937 AA.
AC   P15484; P15485; P15486; P15487; P97157; P97158;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   17-JUN-2020, entry version 85.
DE   RecName: Full=Outer membrane usher protein CS3-2;
DE   AltName: Full=CS3 pili synthesis 104 kDa protein;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PB176 / ETEC;
RX   PubMed=2576094; DOI=10.1111/j.1365-2958.1989.tb00154.x;
RA   Jalajakumari M.B., Thomas C.J., Halter R., Manning P.A.;
RT   "Genes for biosynthesis and assembly of CS3 pili of CFA/II enterotoxigenic
RT   Escherichia coli: novel regulation of pilus production by bypassing an
RT   amber codon.";
RL   Mol. Microbiol. 3:1685-1695(1989).
CC   -!- FUNCTION: These proteins are essential for the biogenesis of mature CS3
CC       pili, but not for synthesis of the CS3 pilin subunit.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=5;
CC       Name=104 kDa;
CC         IsoId=P15484-1; Sequence=Displayed;
CC       Name=63 kDa;
CC         IsoId=P15484-2; Sequence=VSP_018728;
CC       Name=48 kDa;
CC         IsoId=P15484-3; Sequence=VSP_018729;
CC       Name=33 kDa;
CC         IsoId=P15484-4; Sequence=VSP_018730;
CC       Name=20 kDa;
CC         IsoId=P15484-5; Sequence=VSP_018731;
CC   -!- PTM: A 97 kDa form of the protein is thought to be due to post-
CC       translational processing of isoform 104 kDa.
CC   -!- MISCELLANEOUS: Suppression of amber codon 754 seems to be amino acid
CC       specific; a supE suppressor (usually Gln) permits function, whereas a
CC       supF suppressor (Tyr) does not.
CC   -!- MISCELLANEOUS: [Isoform 104 kDa]: Requires the suppression or
CC       readthrough of an internal amber codon in position 754.
CC   -!- SIMILARITY: Belongs to the fimbrial export usher family. {ECO:0000305}.
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DR   EMBL; X16944; CAA34816.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X16944; CAA34817.1; -; Genomic_DNA.
DR   EMBL; X16944; CAA34818.1; -; Genomic_DNA.
DR   EMBL; X16944; CAA34819.1; -; Genomic_DNA.
DR   PIR; S78561; S78561.
DR   PRIDE; P15484; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015473; F:fimbrial usher porin activity; IEA:InterPro.
DR   GO; GO:0009297; P:pilus assembly; IEA:InterPro.
DR   Gene3D; 2.60.40.2070; -; 1.
DR   Gene3D; 2.60.40.2610; -; 1.
DR   InterPro; IPR000015; Fimb_usher.
DR   InterPro; IPR018030; Fimbrial_membr_usher_CS.
DR   InterPro; IPR042186; FimD_plug_dom.
DR   InterPro; IPR025949; PapC-like_C.
DR   InterPro; IPR043142; PapC-like_C_sf.
DR   InterPro; IPR025885; PapC_N.
DR   PANTHER; PTHR30451; PTHR30451; 1.
DR   Pfam; PF13953; PapC_C; 1.
DR   Pfam; PF13954; PapC_N; 1.
DR   Pfam; PF00577; Usher; 1.
DR   PROSITE; PS01151; FIMBRIAL_USHER; 1.
PE   3: Inferred from homology;
KW   Alternative initiation; Cell outer membrane; Fimbrium biogenesis; Membrane;
KW   RNA suppression of termination; Transmembrane; Transmembrane beta strand;
KW   Transport.
FT   CHAIN           1..937
FT                   /note="Outer membrane usher protein CS3-2"
FT                   /id="PRO_0000009301"
FT   VAR_SEQ         1..571
FT                   /note="Missing (in isoform 20 kDa)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018731"
FT   VAR_SEQ         1..450
FT                   /note="Missing (in isoform 33 kDa)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018730"
FT   VAR_SEQ         1..316
FT                   /note="Missing (in isoform 48 kDa)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018729"
FT   VAR_SEQ         1..180
FT                   /note="Missing (in isoform 63 kDa)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018728"
SQ   SEQUENCE   937 AA;  104152 MW;  6C53056EE96277B5 CRC64;
     MYFDAGESED FCIQYSVLQD IGVTVSGNQD ECANLDDELN LRTRFDFYSK RMDIFVSPKF
     VPRKKNGLAP IKLWDEGENA LFTSYNFSED YYHFKGDARD SYSQYANIQP RLNIGPWRIR
     TQAIWNKNNN TKGEWSNNYL YAERGLGNIK SRLYIGDGYF PLKNFNSFKF KGGVLKTDEN
     MYPYSEKTYS PIVKGSAKTQ AKVEFFQDGV KIYSSIVPPG DFSISDYILS GSNSDLYVKV
     IEENGSIQEF IVPFTYPAVA VREGFTYYEI AMGETQQSND YFTQLSFTRG LPYDFTVLTS
     LEYSGFYRSL EIGLGKMLGN LGALSLIYGQ SNFSKSDNSK NKKWDIRYNK NIPDLNTYLS
     FSAVSQTRGG YSSLRDALDY EIGEYTFNSK NSYTASINHS LGELGSLNFS GTWRNYWENK
     NQTRSYNLSY STQIFNGKAY LSGSLIRSEL MNFNNKISDT ILNIGVNIPF GLSRGIQSVS
     YNTSSVKGGR STHQLGISGS EFDNKLYWHV NQGYSDNYSN TSMYGYYKAK YAQVNAGYSV
     SERYNHAYGG IEGGILVYDG GIILGRNLGD TMSIIEAPGA ENTKIRGWGS IETDWRGRAF
     IGYLSPYQNN DISLDPSSLP LDSSLDITTN SVIPTTGAIV KTTYNVKKGK KVMLTLKKSN
     GDAVPFGAIV TVMDGDQNTS IVGDNGQLYL GSSMDTGRLK VIWGNGEDKK CVVDYIVGDN
     KNIAGIYIGS AEHVFSSMLL YGKKISFLSA SVWXVIGVVK AFLTTLQSNN EWRTHSEKND
     FSIDFDVGVG RSFAAVGPTK DMSLGANLTS EPTLAIDFTP IENIYVGANY GKDIGTLVFT
     TNDLTDITLM SSRSVVDGRQ TGFFTFMDSS ATYKISTKLG SSNDVNIQEI TQGAKITPVS
     GEKTLPKKFT LKLHAHRSSS TVPDTYTVGL NVTSNVI