CS3HD_SACS2
ID CS3HD_SACS2 Reviewed; 239 AA.
AC Q97WW0;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=CRISPR-associated endonuclease Cas3-HD;
DE EC=3.1.-.-;
GN Name=cas3; Synonyms=cas3''; OrderedLocusNames=SSO2001;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [2]
RP FUNCTION AS AN ENDONUCLEASE, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, AND
RP MUTAGENESIS OF ASP-63; GLU-92 AND SER-95.
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=19174159; DOI=10.1016/j.febslet.2009.01.024;
RA Han D., Krauss G.;
RT "Characterization of the endonuclease SSO2001 from Sulfolobus solfataricus
RT P2.";
RL FEBS Lett. 583:771-776(2009).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3
CC plus Cascade participate in CRISPR interference, the third stage of
CC CRISPR immunity. Acts as a dsDNA and dsRNA endonuclease (in a fusion
CC with Alicyclus caldarius esterase to increase solubility).
CC {ECO:0000269|PubMed:19174159}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000269|PubMed:19174159};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 3 and 7-8. Expressed as a fusion protein to increase
CC stability. {ECO:0000269|PubMed:19174159};
CC Temperature dependence:
CC Optimum temperature is 50 degrees Celsius. Expressed as a fusion
CC protein to increase stability. {ECO:0000269|PubMed:19174159};
CC -!- DOMAIN: Proteins of this family have an N-terminal nuclease domain and
CC a C-terminal helicase/ATPase domain. In some CRISPR/Cas systems the
CC domains are swapped, in others they are encoded separately.
CC -!- SIMILARITY: Belongs to the CRISPR-associated nuclease Cas3-HD family.
CC {ECO:0000305}.
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DR EMBL; AE006641; AAK42190.1; -; Genomic_DNA.
DR PIR; G90366; G90366.
DR RefSeq; WP_010923717.1; NC_002754.1.
DR AlphaFoldDB; Q97WW0; -.
DR SMR; Q97WW0; -.
DR STRING; 273057.SSO2001; -.
DR DNASU; 1453517; -.
DR EnsemblBacteria; AAK42190; AAK42190; SSO2001.
DR GeneID; 27428326; -.
DR KEGG; sso:SSO2001; -.
DR PATRIC; fig|273057.12.peg.2077; -.
DR eggNOG; arCOG01442; Archaea.
DR HOGENOM; CLU_1163752_0_0_2; -.
DR InParanoid; Q97WW0; -.
DR OMA; DYTFDDY; -.
DR BRENDA; 3.1.99.B5; 6163.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3210.30; -; 1.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR TIGRFAMs; TIGR01596; cas3_HD; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW Nuclease; Reference proteome.
FT CHAIN 1..239
FT /note="CRISPR-associated endonuclease Cas3-HD"
FT /id="PRO_0000417608"
FT DOMAIN 5..195
FT /note="HD Cas3-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00974"
FT BINDING 63
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 92
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255"
FT MUTAGEN 63
FT /note="D->A: Significant loss of endonuclease activity.
FT Complete loss of activity; when associated with A-92."
FT /evidence="ECO:0000269|PubMed:19174159"
FT MUTAGEN 92
FT /note="E->A: Some loss of endonuclease activity. Complete
FT loss of activity; when associated with A-63."
FT /evidence="ECO:0000269|PubMed:19174159"
FT MUTAGEN 95
FT /note="S->A: No effect."
FT /evidence="ECO:0000269|PubMed:19174159"
SQ SEQUENCE 239 AA; 27515 MW; 1BE0167CDE783D42 CRC64;
MIKPCAYEKQ GLIDHAIGSY RVLDGKISES YYKIISRRLE RYGIVLDLNG VKEMVKDVVV
LHDMGKAGEY YQNQFDDNCN PLKSNFSFIY HELGSALFFY YDYEPIDVEK AEEVKSLLTL
AVLNHLNAIR VISDYLVNKF PDNFDERMIK LNKYGSIMLQ NLRGVISKSL KVRDYTFDDY
HDMLYAFSKK SDKYLKLYNL FLAPIMLGDN LDSSLVRNNG SKTGFVRILE GELNGGSTL
