ID   CSA2A_SACS2             Reviewed;         318 AA.
AC   Q97YC7;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=CRISPR-associated protein Cas7/Csa2 1;
DE   AltName: Full=CRISPR-associated aCascade subunit Cas7/Csa2, subtype I-A/Apern 1;
GN   Name=cas7a; OrderedLocusNames=SSO1399;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2]
RP   SUBUNIT.
RC   STRAIN=ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1;
RX   PubMed=21507944; DOI=10.1074/jbc.m111.238485;
RA   Lintner N.G., Kerou M., Brumfield S.K., Graham S., Liu H., Naismith J.H.,
RA   Sdano M., Peng N., She Q., Copie V., Young M.J., White M.F., Lawrence C.M.;
RT   "Structural and functional characterization of an archaeal clustered
RT   regularly interspaced short palindromic repeat (CRISPR)-associated complex
RT   for antiviral defense (CASCADE).";
RL   J. Biol. Chem. 286:21643-21656(2011).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the aCascade ribonucleoprotein complex, minimally
CC       composed of Csa2 and Cas5a, which binds crRNA. Other possible
CC       components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5
CC       (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes
CC       been seen weakly associated. Csa2 is probably the major RNA-binding
CC       subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous
CC       to crRNA, probably forming an R-loop. Purified aCascade forms a
CC       filament about 6 nm in width. {ECO:0000269|PubMed:21507944}.
CC   -!- MISCELLANEOUS: The aCascade complex was purified from strain P1.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas7/Cst2/DevR
CC       family. Subtype I-a/Apern subfamily. {ECO:0000305}.
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DR   EMBL; AE006641; AAK41634.1; -; Genomic_DNA.
DR   PIR; C99297; C99297.
DR   AlphaFoldDB; Q97YC7; -.
DR   SMR; Q97YC7; -.
DR   STRING; 273057.SSO1399; -.
DR   EnsemblBacteria; AAK41634; AAK41634; SSO1399.
DR   KEGG; sso:SSO1399; -.
DR   PATRIC; fig|273057.12.peg.1415; -.
DR   eggNOG; arCOG03617; Archaea.
DR   HOGENOM; CLU_054331_0_0_2; -.
DR   InParanoid; Q97YC7; -.
DR   OMA; NYARHRT; -.
DR   PhylomeDB; Q97YC7; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR002764; Cas7/Cst2/DevR_sub_I-a/Apern.
DR   InterPro; IPR010154; CRISPR-assoc_Cas7/Cst2/DevR.
DR   Pfam; PF01905; DevR; 1.
DR   TIGRFAMs; TIGR01875; cas_MJ0381; 1.
DR   TIGRFAMs; TIGR02583; DevR_archaea; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Reference proteome.
FT   CHAIN           1..318
FT                   /note="CRISPR-associated protein Cas7/Csa2 1"
FT                   /id="PRO_0000417886"
SQ   SEQUENCE   318 AA;  35852 MW;  6A6C3E459D62BBBD CRC64;
     MQLEVNNMWI SFSVRYLVNV EDLNNVESAG NYVRHRRAPL VFKDKDSYTV TYVPAVSGEM
     IAHGYQMNLV ELALQRNLPV DSLAKQGILI KRGSDDKVHE GTKCTDEKGS DYELCVINED
     IVEDVAGFMN PNKLVKRTSN VAFSYMVPAI DAVKASTISS QFHVRYANKE LMDKYKNENI
     QSLYNIETAS ASYVLTGYLN VNSVGKTQNY PVKEVDKKKD REKAALDALM LTLTQFLFGA
     KLTRFKPIVE IEALFVSASE KPFNLPPVTG DIKKYIDLVN STTDSFAKIL NIKRPVVKYY
     LKEEKGNVNT PIDAFTVM