ID   CSA3_SACS2              Reviewed;         237 AA.
AC   Q97Y88;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=CRISPR locus-related putative DNA-binding protein Csa3;
GN   Name=csa3; OrderedLocusNames=SSO1445;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS), AND SUBUNIT.
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=21093452; DOI=10.1016/j.jmb.2010.11.019;
RA   Lintner N.G., Frankel K.A., Tsutakawa S.E., Alsbury D.L., Copie V.,
RA   Young M.J., Tainer J.A., Lawrence C.M.;
RT   "The structure of the CRISPR-associated protein Csa3 provides insight into
RT   the regulation of the CRISPR/Cas system.";
RL   J. Mol. Biol. 405:939-955(2011).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:21093452}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated Csa3 family.
CC       {ECO:0000305}.
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DR   EMBL; AE006641; AAK41678.1; -; Genomic_DNA.
DR   PIR; G90302; G90302.
DR   RefSeq; WP_010923410.1; NC_002754.1.
DR   PDB; 2WTE; X-ray; 1.80 A; A/B=1-237.
DR   PDB; 6W11; X-ray; 2.46 A; A/B=1-212.
DR   PDBsum; 2WTE; -.
DR   PDBsum; 6W11; -.
DR   AlphaFoldDB; Q97Y88; -.
DR   SMR; Q97Y88; -.
DR   STRING; 273057.SSO1445; -.
DR   DNASU; 1454457; -.
DR   EnsemblBacteria; AAK41678; AAK41678; SSO1445.
DR   GeneID; 27427813; -.
DR   KEGG; sso:SSO1445; -.
DR   PATRIC; fig|273057.12.peg.1476; -.
DR   eggNOG; arCOG01446; Archaea.
DR   HOGENOM; CLU_107894_1_0_2; -.
DR   OMA; GFNETYL; -.
DR   EvolutionaryTrace; Q97Y88; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR010163; Csa3.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   TIGRFAMs; TIGR01884; cas_HTH; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antiviral defense; DNA-binding; Reference proteome.
FT   CHAIN           1..237
FT                   /note="CRISPR locus-related putative DNA-binding protein
FT                   Csa3"
FT                   /id="PRO_0000417889"
FT   STRAND          3..6
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           13..21
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          28..37
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           40..56
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          61..66
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           71..82
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          87..92
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          94..96
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           98..110
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          115..120
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          128..132
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           133..140
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           145..157
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           162..169
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           173..185
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          188..192
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   TURN            193..196
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   STRAND          197..200
FT                   /evidence="ECO:0007829|PDB:2WTE"
FT   HELIX           202..210
FT                   /evidence="ECO:0007829|PDB:2WTE"
SQ   SEQUENCE   237 AA;  26816 MW;  BC4DC274587E7CE2 CRC64;
     MKSYFVTMGF NETFLLRLLN ETSAQKEDSL VIVVPSPIVS GTRAAIESLR AQISRLNYPP
     PRIYEIEITD FNLALSKILD IILTLPEPII SDLTMGMRMI NTLILLGIIV SRKRFTVYVR
     DEGGGSRVIS FNDNTIRALM RDYSREEMKL LNVLYETKGT GITELAKMLD KSEKTLINKI
     AELKKFGILT QKGKDRKVEL NELGLNVIKL NKSVIESSKS SEELVKENKG KEVNIPY