CSC1L_BRAJU
ID CSC1L_BRAJU Reviewed; 723 AA.
AC A9LIW2;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 25-MAY-2022, entry version 22.
DE RecName: Full=CSC1-like protein ERD4;
DE AltName: Full=Protein EARLY-RESPONSIVE TO DEHYDRATION STRESS 4;
GN Name=ERD4;
OS Brassica juncea (Indian mustard) (Sinapis juncea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3707;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. CS52;
RA Archana K., Patade V.Y., Suprasanna P.;
RT "Cloning and characterization of early responsive to dehydration 4 gene
RT from Indian mustard (Brassica juncea).";
RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=22431979; DOI=10.1371/journal.pone.0032658;
RA Rai A., Suprasanna P., D'Souza S.F., Kumar V.;
RT "Membrane topology and predicted RNA-binding function of the 'early
RT responsive to dehydration (ERD4)' plant protein.";
RL PLoS ONE 7:E32658-E32658(2012).
CC -!- FUNCTION: Acts as an osmosensitive calcium-permeable cation channel.
CC {ECO:0000250, ECO:0000269|PubMed:22431979}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000305|PubMed:22431979}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:22431979}.
CC -!- SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to possess 2 RRM (RNA recognition
CC motif) domains and to bind RNA (PubMed:22431979). It seems to be in
CC disagreement with the supposed ion transporter function.
CC {ECO:0000305|PubMed:22431979}.
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DR EMBL; EU126607; ABX56139.2; -; Genomic_DNA.
DR AlphaFoldDB; A9LIW2; -.
DR SMR; A9LIW2; -.
DR GO; GO:0031969; C:chloroplast membrane; TAS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005227; F:calcium activated cation channel activity; IEA:InterPro.
DR InterPro; IPR045122; Csc1-like.
DR InterPro; IPR032880; Csc1_N.
DR InterPro; IPR027815; PHM7_cyt.
DR InterPro; IPR003864; RSN1_7TM.
DR PANTHER; PTHR13018; PTHR13018; 1.
DR Pfam; PF14703; PHM7_cyt; 1.
DR Pfam; PF02714; RSN1_7TM; 1.
DR Pfam; PF13967; RSN1_TM; 1.
PE 3: Inferred from homology;
KW Calcium; Chloroplast; Ion channel; Ion transport; Membrane; Plastid;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..723
FT /note="CSC1-like protein ERD4"
FT /id="PRO_0000429813"
FT TOPO_DOM 1..5
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..90
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..364
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 386..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 438..456
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 478..508
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 509..529
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 530..572
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 573..593
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 594..614
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 615..635
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 636..637
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 638..658
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 659..723
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 723 AA; 81931 MW; 1411FD7763C08C42 CRC64;
MEFASFLVSL GTSAIIFVVL MFLFTWLSRR PGNVPVYYPN RILKGMDPWE GSSLTRNPFA
WIREAFTSTE QDVVKLSGVD TAVYFVFQST VLGIFALSAL LLLPTLLPIA ATDNNLETSR
SATDTTSNGT FSQLDNLSMA NITKSSSRLW AFLGAVYWVS VVTYFMLWKA YKHVAALRAQ
ALMTSEEVLP EQFAILVRDI PSPPNGETQK EFVDSYFRDI YPETFYRSLV VTENSKINKI
WEDLEGYKKK LARAEAAFAA TSNRPTNKTG LLGLVGERVD SIDYYTKLIN ESVAKLEAEQ
RTVLAERQQT AAVVFFTDRV TAALAAQSLH CQMVDKWTVT EAPEPRQLIW ENLKIKFFSR
IVRQYVIYFL VAITILFYMI PIAFVSAITT LANLQKALPF LKPIVDIAFI RTILESYLPQ
IALIVFLAML PKFLMFLSKS EGIPSQSHAI RATSGKYFYF SVLNVFIGVT LAGSLFENLK
ALEEKPNSFI TLLATSLPKS ATFFLTYVAL KFFVGYGLEL SRIIPLIIFH LKKKYLCKTE
AEVKEAWYPG DLSYATRVPS DMLILTITFC YSVIAPLILV FGVIYFGLGW LILRNQALKV
YVPSYESYGR MWPHIHTRIL AALFLFQLVM FGYLGVKIFV WAILLVPLIF ISLIFGYVCR
QKFYGGFEHT ALEVACRELK QRPDLEEVFR AYIPHSLSTH KGDDHQFKGA MSRYQDYAAI
SAA
