CSC1_MOUSE
ID CSC1_MOUSE Reviewed; 802 AA.
AC Q8CBX0;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Calcium permeable stress-gated cation channel 1;
DE AltName: Full=Transmembrane protein 63C;
GN Name=Tmem63c; Synonyms=CSC1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Diencephalon;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75 AND SER-78, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=27045885; DOI=10.1002/cbf.3185;
RA Zhao X., Yan X., Liu Y., Zhang P., Ni X.;
RT "Co-expression of mouse TMEM63A, TMEM63B and TMEM63C confers
RT hyperosmolarity activated ion currents in HEK293 cells.";
RL Cell Biochem. Funct. 34:238-241(2016).
CC -!- FUNCTION: Acts as an osmosensitive calcium-permeable cation channel
CC (PubMed:27045885). Required for the functional integrity of the kidney
CC glomerular filtration barrier (By similarity).
CC {ECO:0000250|UniProtKB:D3ZNF5, ECO:0000269|PubMed:27045885}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:27045885};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. {ECO:0000305}.
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DR EMBL; AK034410; BAC28698.1; -; mRNA.
DR EMBL; BC056936; AAH56936.1; -; mRNA.
DR EMBL; BC057088; AAH57088.1; -; mRNA.
DR CCDS; CCDS26069.1; -.
DR RefSeq; NP_766171.1; NM_172583.2.
DR RefSeq; XP_006515779.1; XM_006515716.3.
DR RefSeq; XP_006515780.1; XM_006515717.3.
DR RefSeq; XP_006515781.1; XM_006515718.3.
DR RefSeq; XP_006515783.1; XM_006515720.2.
DR RefSeq; XP_011242383.1; XM_011244081.2.
DR RefSeq; XP_011242384.1; XM_011244082.2.
DR RefSeq; XP_011242385.1; XM_011244083.2.
DR RefSeq; XP_011242386.1; XM_011244084.2.
DR RefSeq; XP_011242387.1; XM_011244085.2.
DR RefSeq; XP_011242388.1; XM_011244086.2.
DR AlphaFoldDB; Q8CBX0; -.
DR SMR; Q8CBX0; -.
DR STRING; 10090.ENSMUSP00000119872; -.
DR iPTMnet; Q8CBX0; -.
DR PhosphoSitePlus; Q8CBX0; -.
DR SwissPalm; Q8CBX0; -.
DR PaxDb; Q8CBX0; -.
DR PRIDE; Q8CBX0; -.
DR ProteomicsDB; 285340; -.
DR Antibodypedia; 25977; 25 antibodies from 14 providers.
DR DNASU; 217733; -.
DR Ensembl; ENSMUST00000110187; ENSMUSP00000105816; ENSMUSG00000034145.
DR Ensembl; ENSMUST00000131878; ENSMUSP00000117023; ENSMUSG00000034145.
DR Ensembl; ENSMUST00000146292; ENSMUSP00000119872; ENSMUSG00000034145.
DR GeneID; 217733; -.
DR KEGG; mmu:217733; -.
DR UCSC; uc007oig.1; mouse.
DR CTD; 57156; -.
DR MGI; MGI:2444386; Tmem63c.
DR VEuPathDB; HostDB:ENSMUSG00000034145; -.
DR eggNOG; KOG1134; Eukaryota.
DR GeneTree; ENSGT00940000159072; -.
DR HOGENOM; CLU_015647_0_0_1; -.
DR InParanoid; Q8CBX0; -.
DR OMA; QGIGMFP; -.
DR OrthoDB; 395194at2759; -.
DR PhylomeDB; Q8CBX0; -.
DR TreeFam; TF324300; -.
DR BioGRID-ORCS; 217733; 1 hit in 74 CRISPR screens.
DR ChiTaRS; Tmem63c; mouse.
DR PRO; PR:Q8CBX0; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q8CBX0; protein.
DR Bgee; ENSMUSG00000034145; Expressed in dentate gyrus of hippocampal formation granule cell and 49 other tissues.
DR ExpressionAtlas; Q8CBX0; baseline and differential.
DR Genevisible; Q8CBX0; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0005227; F:calcium activated cation channel activity; ISO:MGI.
DR GO; GO:1990760; F:osmolarity-sensing cation channel activity; IDA:UniProtKB.
DR GO; GO:0006812; P:cation transport; ISO:MGI.
DR GO; GO:0003094; P:glomerular filtration; ISS:UniProtKB.
DR InterPro; IPR045122; Csc1-like.
DR InterPro; IPR032880; Csc1_N.
DR InterPro; IPR027815; PHM7_cyt.
DR InterPro; IPR003864; RSN1_7TM.
DR PANTHER; PTHR13018; PTHR13018; 1.
DR Pfam; PF14703; PHM7_cyt; 1.
DR Pfam; PF02714; RSN1_7TM; 1.
DR Pfam; PF13967; RSN1_TM; 1.
PE 1: Evidence at protein level;
KW Calcium; Cell membrane; Ion channel; Ion transport; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..802
FT /note="Calcium permeable stress-gated cation channel 1"
FT /id="PRO_0000280731"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..514
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 540..560
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 604..624
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 655..675
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 685..705
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 753..785
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 753..772
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 78
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 802 AA; 93011 MW; 861020EE3B05EA5A CRC64;
MSAFPDSMDQ KFHNMTVNEC FQSRSTVLQG QPFGGIPTVL VLNIILWVFV VLLYSFLRKA
AWDYGRLALL IHNDSLTSLI YGEQSEKSSP SEVSLEAERR DRGFSSWFFN SLTMRDRDLI
NKCGDDARIY ITFQYHLIIF VLILCIPSLG IILPVNYIGT VLDWNSHFGR TTIVNVSTES
KFLWLHSLFA FLYFLINLAF MGHHCLGFVP KKSLHFTRTL MITYVPTEIQ DPEIISKHFH
EAYPGCVVTR VHFCYDVRNL IDLDDQRRHA MRGRLYYTAK AKKTGKVMIK THPCSRLCFC
KCWTCFKEVD AEQYYSELEE QLTDEFNAEL NRVQLKRLDL IFVTFQDART VRRIYDDYKY
IHCGRHPKQS SVTTIVKNYH WRVAHAPHPK DIIWKHLSIR RFSWWTRFIA INTFLFFLFF
FLTTPAIIIN TIDIYNVTRP IEKLQSPIVT QFFPSVLLWA FTVTMPLLVY LSAFLEAHWT
RSSQNLIIVH KCYIFLVFMV VILPSMGLTS LHVFLRWLFD IYYLEHATIR FQCVFLPDNG
AFFINYVITA ALLGTGMELM RLGSLCTYCT RLFLSKSEPE RVHIRKNQAT DFQFGREYAW
MLNVFSVVMA YSITCPIIVP FGLLYLCMKH ITDRYNMYYS YAPTKLNAQI HMAAVYQAIF
APLLGLFWML FFSILRVGSL HSITLFSMSS LIISVVIAFS GVFLGKLRIA QRYEQPEEET
ETVFDVEPSS TTSTPTSLLY VATVLQEPEL NLTPASSPAR HTYGTINSQP EEGEEESGLR
GFARELDSAQ FQEGLEMEGQ SH
