CSCB_ECOLX
ID CSCB_ECOLX Reviewed; 415 AA.
AC P30000;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Sucrose permease {ECO:0000303|PubMed:7535526};
DE AltName: Full=Sucrose transport protein {ECO:0000305};
GN Name=cscB {ECO:0000303|PubMed:1435727};
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=EC3132;
RX PubMed=1435727; DOI=10.1007/bf00286177;
RA Bockmann J., Heuel H., Lengeler J.W.;
RT "Characterization of a chromosomally encoded, non-PTS metabolic pathway for
RT sucrose utilization in Escherichia coli EC3132.";
RL Mol. Gen. Genet. 235:22-32(1992).
RN [2]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
RX PubMed=7535526; DOI=10.1006/bbrc.1995.1449;
RA Sahin-Toth M., Frillingos S., Lengeler J.W., Kaback H.R.;
RT "Active transport by the CscB permease in Escherichia coli K-12.";
RL Biochem. Biophys. Res. Commun. 208:1116-1123(1995).
RN [3]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=19294451; DOI=10.1007/s00232-009-9161-9;
RA Peng Y., Kumar S., Hernandez R.L., Jones S.E., Cadle K.M., Smith K.P.,
RA Varela M.F.;
RT "Evidence for the transport of maltose by the sucrose permease, CscB, of
RT Escherichia coli.";
RL J. Membr. Biol. 228:79-88(2009).
RN [4]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=22106930; DOI=10.1021/bi201592y;
RA Sugihara J., Smirnova I., Kasho V., Kaback H.R.;
RT "Sugar recognition by CscB and LacY.";
RL Biochemistry 50:11009-11014(2011).
CC -!- FUNCTION: Responsible for transport of sucrose into the cell, with the
CC concomitant import of a proton (symport system) (PubMed:7535526,
CC PubMed:22106930). Can also transport maltose, fructose or lactulose,
CC but not glucose, lactose or melibiose (PubMed:19294451, PubMed:7535526,
CC PubMed:22106930). The substrate specificity is directed toward the
CC fructofuranosyl moiety of the substrate (PubMed:22106930).
CC {ECO:0000269|PubMed:19294451, ECO:0000269|PubMed:22106930,
CC ECO:0000269|PubMed:7535526}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1 mM for sucrose {ECO:0000269|PubMed:7535526};
CC KM=0.37 mM for sucrose {ECO:0000269|PubMed:19294451};
CC KM=0.59 mM for maltose {ECO:0000269|PubMed:19294451};
CC KM=6.7 mM for sucrose {ECO:0000269|PubMed:22106930};
CC KM=36 mM for fructose {ECO:0000269|PubMed:22106930};
CC Vmax=23 nmol/min/mg enzyme with sucrose as substrate
CC {ECO:0000269|PubMed:7535526};
CC Vmax=83 nmol/min/mg enzyme with sucrose as substrate
CC {ECO:0000269|PubMed:19294451};
CC Vmax=111 nmol/min/mg enzyme with maltose as substrate
CC {ECO:0000269|PubMed:19294451};
CC Vmax=130 nmol/min/mg enzyme with sucrose as substrate
CC {ECO:0000269|PubMed:22106930};
CC Vmax=60 nmol/min/mg enzyme with fructose as substrate
CC {ECO:0000269|PubMed:22106930};
CC -!- PATHWAY: Glycan biosynthesis; sucrose metabolism.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:7535526};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Oligosaccharide:H(+) symporter (OHS) (TC 2.A.1.5) family.
CC {ECO:0000305}.
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DR EMBL; X63740; CAA45274.1; -; Genomic_DNA.
DR EMBL; X81461; CAA57217.1; -; Genomic_DNA.
DR PIR; S30107; GRECST.
DR RefSeq; WP_001197021.1; NZ_WVUW01000001.1.
DR AlphaFoldDB; P30000; -.
DR SMR; P30000; -.
DR STRING; 585034.ECIAI1_2426; -.
DR TCDB; 2.A.1.5.3; the major facilitator superfamily (mfs).
DR OrthoDB; 791272at2; -.
DR UniPathway; UPA00238; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IEA:InterPro.
DR GO; GO:0005985; P:sucrose metabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR000576; LacY/RafB_perm_fam.
DR InterPro; IPR018457; LacY/RafB_perm_fam_CS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF01306; LacY_symp; 1.
DR PRINTS; PR00174; LACYSMPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00882; 2A0105; 1.
DR PROSITE; PS00896; LACY_1; 1.
DR PROSITE; PS00897; LACY_2; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..415
FT /note="Sucrose permease"
FT /id="PRO_0000196188"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..48
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..77
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..107
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..147
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 148..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..170
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 171..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 191..220
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..260
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 282..287
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..311
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 333..342
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..377
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..415
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
SQ SEQUENCE 415 AA; 46924 MW; D05BBD305B61AC22 CRC64;
MALNIPFRNA YYRFASSYSF LFFISWSLWW SLYAIWLKGH LGLTGTELGT LYSVNQFTSI
LFMMFYGIVQ DKLGLKKPLI WCMSFILVLT GPFMIYVYEP LLQSNFSVGL ILGALFFGLG
YLAGCGLLDS FTEKMARNFH FEYGTARAWG SFGYAIGAFF AGIFFSISPH INFWLVSLFG
AVFMMINMRF KDKDHQCIAA DAGGVKKEDF IAVFKDRNFW VFVIFIVGTW SFYNIFDQQL
FPVFYAGLFE SHDVGTRLYG YLNSFQVVLE ALCMAIIPFF VNRVGPKNAL LIGVVIMALR
ILSCALFVNP WIISLVKLLH AIEVPLCVIS VFKYSVANFD KRLSSTIFLI GFQIASSLGI
VLLSTPTGIL FDHAGYQTVF FAISGIVCLM LLFGIFFLSK KREQIVMETP VPSAI
