ID   CSCL2_ARATH             Reviewed;         785 AA.
AC   Q9SY14; Q9ZT88;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=CSC1-like protein At4g02900;
GN   OrderedLocusNames=At4g02900; ORFNames=T4I9.22, T5J8.22;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=24503647; DOI=10.1038/cr.2014.14;
RA   Hou C., Tian W., Kleist T., He K., Garcia V., Bai F., Hao Y., Luan S.,
RA   Li L.;
RT   "DUF221 proteins are a family of osmosensitive calcium-permeable cation
RT   channels conserved across eukaryotes.";
RL   Cell Res. 24:632-635(2014).
CC   -!- FUNCTION: Acts as an osmosensitive calcium-permeable cation channel.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC79116.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC004044; AAD15333.1; -; Genomic_DNA.
DR   EMBL; AF069442; AAC79116.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161495; CAB77775.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE82248.1; -; Genomic_DNA.
DR   PIR; H85036; H85036.
DR   PIR; T01403; T01403.
DR   RefSeq; NP_192199.1; NM_116524.2.
DR   AlphaFoldDB; Q9SY14; -.
DR   SMR; Q9SY14; -.
DR   STRING; 3702.AT4G02900.1; -.
DR   TCDB; 1.A.17.5.9; the calcium-dependent chloride channel (ca-clc) family.
DR   PaxDb; Q9SY14; -.
DR   PRIDE; Q9SY14; -.
DR   ProteomicsDB; 220495; -.
DR   EnsemblPlants; AT4G02900.1; AT4G02900.1; AT4G02900.
DR   GeneID; 828143; -.
DR   Gramene; AT4G02900.1; AT4G02900.1; AT4G02900.
DR   KEGG; ath:AT4G02900; -.
DR   Araport; AT4G02900; -.
DR   TAIR; locus:2140210; AT4G02900.
DR   eggNOG; KOG1134; Eukaryota.
DR   HOGENOM; CLU_002458_7_1_1; -.
DR   InParanoid; Q9SY14; -.
DR   OrthoDB; 395194at2759; -.
DR   PhylomeDB; Q9SY14; -.
DR   PRO; PR:Q9SY14; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9SY14; baseline and differential.
DR   Genevisible; Q9SY14; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005227; F:calcium activated cation channel activity; IBA:GO_Central.
DR   InterPro; IPR045122; Csc1-like.
DR   InterPro; IPR032880; Csc1_N.
DR   InterPro; IPR027815; PHM7_cyt.
DR   InterPro; IPR003864; RSN1_7TM.
DR   PANTHER; PTHR13018; PTHR13018; 1.
DR   Pfam; PF14703; PHM7_cyt; 1.
DR   Pfam; PF02714; RSN1_7TM; 1.
DR   Pfam; PF13967; RSN1_TM; 1.
PE   3: Inferred from homology;
KW   Calcium; Ion channel; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..785
FT                   /note="CSC1-like protein At4g02900"
FT                   /id="PRO_0000429800"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        156..176
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..445
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        465..485
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        510..530
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        582..602
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        628..648
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        651..671
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          725..761
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        735..761
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   785 AA;  89779 MW;  5D4924DE51072905 CRC64;
     MASVQDIGLS AAINLLSAFA FLFAFAMLRL QPVNDRVYFP KWYLKGIRGS PTRSRGIMTR
     FVNLDWTTYV KFLNWMPAAL QMPEPELIEH AGLDSAVYIR IYLLGLKMFV PITLLAFGVL
     VPVNWTGETL ENIDDLTFSN VDKLSISNVP PGSPRFWAHI TMTYVITFWT CYILYMEYKA
     VANMRLRHLA AESRRPDQLT VLVRNVPPDP DESVNEHVEH FFCVNHPDHY LCHQVVYNAN
     DLAKLVAQRK AMQNWLTYYE NKFERKPSSR PTTKTGYGGF WGTTVDAIDF YTSKMDILAE
     QEAVEREKIM NDPKAIMPAA FVSFRSRWGT AVCAQTQQCH NPTIWLTEWA PEPRDVFWDN
     LAIPYVELSI RRLLTTVALF FLIFCFMIPI AFVQSLANLE GIQKVLPFLK PVIEMKTVKS
     VIQGFLPGIA LKIFLIILPT ILMTMSQIEG YTSLSYLDRR SAEKYFWFII VNVFLGSIIT
     GTAFQQLKSF LEQPPTEIPK TVGVSIPMKA TFFITYIMVD GWAGIAAEIL RVVPLVIFHL
     KNTFLVKTEQ DRQQAMDPGH LDFATSEPRI QFYFLLGLVY AAVAPILLPF IIVFFAFAYV
     VFRHQVINVY DQKYESGARY WPDVHRRLII CLIISQLLMM GLLSTKKFAK VTALLLPQPI
     LTFWFYRYCA GRFESAFSKF PLQEAMVKDT LEKATEPNLN LKEYLKDAYV HPVFKGNDFD
     RPRVVDEEES NPLVRTKRTS QGTTRYNSEA SSSATTTPVA NNDSPRCWGT KIGSFGCVIV
     VSYSC