ID   CSCLB_ARATH             Reviewed;         711 AA.
AC   F4I248; Q9C792;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=CSC1-like protein At1g69450;
GN   OrderedLocusNames=At1g69450; ORFNames=F10D13_27;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-406.
RC   STRAIN=cv. Columbia;
RX   PubMed=14993207; DOI=10.1101/gr.1515604;
RA   Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA   Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA   Weissenbach J., Salanoubat M.;
RT   "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT   combined approach to evaluate and improve Arabidopsis genome annotation.";
RL   Genome Res. 14:406-413(2004).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=24503647; DOI=10.1038/cr.2014.14;
RA   Hou C., Tian W., Kleist T., He K., Garcia V., Bai F., Hao Y., Luan S.,
RA   Li L.;
RT   "DUF221 proteins are a family of osmosensitive calcium-permeable cation
RT   channels conserved across eukaryotes.";
RL   Cell Res. 24:632-635(2014).
CC   -!- FUNCTION: Acts as an osmosensitive calcium-permeable cation channel.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG60099.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC073178; AAG60099.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE34926.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE34927.1; -; Genomic_DNA.
DR   EMBL; BX814798; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_001185356.1; NM_001198427.2.
DR   RefSeq; NP_177104.2; NM_105612.2.
DR   AlphaFoldDB; F4I248; -.
DR   SMR; F4I248; -.
DR   STRING; 3702.AT1G69450.2; -.
DR   PaxDb; F4I248; -.
DR   PRIDE; F4I248; -.
DR   ProteomicsDB; 220493; -.
DR   EnsemblPlants; AT1G69450.1; AT1G69450.1; AT1G69450.
DR   EnsemblPlants; AT1G69450.2; AT1G69450.2; AT1G69450.
DR   GeneID; 843277; -.
DR   Gramene; AT1G69450.1; AT1G69450.1; AT1G69450.
DR   Gramene; AT1G69450.2; AT1G69450.2; AT1G69450.
DR   KEGG; ath:AT1G69450; -.
DR   Araport; AT1G69450; -.
DR   TAIR; locus:2007126; AT1G69450.
DR   eggNOG; KOG1134; Eukaryota.
DR   HOGENOM; CLU_002458_7_1_1; -.
DR   InParanoid; F4I248; -.
DR   OMA; ILDPRVY; -.
DR   PRO; PR:F4I248; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4I248; baseline and differential.
DR   Genevisible; F4I248; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005227; F:calcium activated cation channel activity; IBA:GO_Central.
DR   InterPro; IPR045122; Csc1-like.
DR   InterPro; IPR032880; Csc1_N.
DR   InterPro; IPR027815; PHM7_cyt.
DR   InterPro; IPR003864; RSN1_7TM.
DR   PANTHER; PTHR13018; PTHR13018; 1.
DR   Pfam; PF14703; PHM7_cyt; 1.
DR   Pfam; PF02714; RSN1_7TM; 1.
DR   Pfam; PF13967; RSN1_TM; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Ion channel; Ion transport; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..711
FT                   /note="CSC1-like protein At1g69450"
FT                   /id="PRO_0000429809"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        351..371
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        403..423
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        488..510
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        553..573
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        595..615
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        620..640
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   711 AA;  81790 MW;  ADC70C48A97580B5 CRC64;
     MLLSALLMSV GINSCLCVLL FILYSVLRKQ PRNYEVFLPR RLANGTYKRR RNKVARYIPS
     LKWIWKSWRP TEKELMESSG LDGVVFMRMI TFSLKVFLFA GIIGVFVLLP VNCFGDQLTV
     IDYADWSANS LDLFSVANLK VRSQWLWVHF GAIYLVTVFV CCLLYFEFRY IALKRIEHFY
     SSKPKPEQFT ILVRNIPSSD GSSVSDTVDR FFGENHSSTY FSHVVIHRTS KLRSVVDKAK
     KLYKEVKHKK PVKKTPMRFF SRKDNTEGHY ESVLQEMEQN IRLGQAEVSA PGKEVRAAFV
     SFKSRYGAAT ALHMPQSINP TYWLTEPAPE PHDVHWPFFS ASFMQKWLAK ILVVFACLLL
     TILFLVPVVL VQGLTNLPAL EFMFPFLSLI LSMKVVSQII TGYLPSLILQ TSLKVVPPTM
     EFLSSIQGHI CHSDIQKSAC NKVIWFTIWN VFFATVFSGS AFYKLSVILD PKQIPLKLAV
     AVPAQASFFI AYVVTTGWTD TLTELFRVVP FMVSYIKRSF EPSDENEFVV PPMRYHRDTP
     RVLFFGLLGI TYFFLAPLIL PFILLYFILA YIIYRNQFMN VYAPKFDTGG MFWPMIHYTM
     IFSLVLMQAI AIGLFALKKM ELATYLLVPL PVFTLLFNEF CRKRFMPIFT DYPAEVLTKR
     DKEDRNDPTM PEFYNNLVSA YKDPALLPLR FSGSGSRNDS LTSPLLSFSE V