CSCLE_ARATH
ID CSCLE_ARATH Reviewed; 817 AA.
AC Q9SZT4;
DT 09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=CSC1-like protein At4g35870;
GN Name=GFS10; OrderedLocusNames=At4g35870; ORFNames=F4B14_140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=17293568; DOI=10.1105/tpc.106.045997;
RA Fuji K., Shimada T., Takahashi H., Tamura K., Koumoto Y., Utsumi S.,
RA Nishizawa K., Maruyama N., Hara-Nishimura I.;
RT "Arabidopsis vacuolar sorting mutants (green fluorescent seed) can be
RT identified efficiently by secretion of vacuole-targeted green fluorescent
RT protein in their seeds.";
RL Plant Cell 19:597-609(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=24503647; DOI=10.1038/cr.2014.14;
RA Hou C., Tian W., Kleist T., He K., Garcia V., Bai F., Hao Y., Luan S.,
RA Li L.;
RT "DUF221 proteins are a family of osmosensitive calcium-permeable cation
RT channels conserved across eukaryotes.";
RL Cell Res. 24:632-635(2014).
CC -!- FUNCTION: Acts as an osmosensitive calcium-permeable cation channel.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Accumulated 12S globulin precursors in seeds.
CC {ECO:0000269|PubMed:17293568}.
CC -!- SIMILARITY: Belongs to the CSC1 (TC 1.A.17) family. {ECO:0000305}.
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DR EMBL; AL031986; CAA21473.1; -; Genomic_DNA.
DR EMBL; AL161588; CAB81496.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86583.1; -; Genomic_DNA.
DR EMBL; AY090251; AAL90913.1; -; mRNA.
DR EMBL; AY096495; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T04697; T04697.
DR RefSeq; NP_195312.1; NM_119753.3.
DR AlphaFoldDB; Q9SZT4; -.
DR STRING; 3702.AT4G35870.1; -.
DR PaxDb; Q9SZT4; -.
DR PRIDE; Q9SZT4; -.
DR ProteomicsDB; 220353; -.
DR EnsemblPlants; AT4G35870.1; AT4G35870.1; AT4G35870.
DR GeneID; 829741; -.
DR Gramene; AT4G35870.1; AT4G35870.1; AT4G35870.
DR KEGG; ath:AT4G35870; -.
DR Araport; AT4G35870; -.
DR TAIR; locus:2125394; AT4G35870.
DR eggNOG; KOG1134; Eukaryota.
DR HOGENOM; CLU_007889_0_0_1; -.
DR InParanoid; Q9SZT4; -.
DR OMA; EYFQHKY; -.
DR OrthoDB; 395194at2759; -.
DR PhylomeDB; Q9SZT4; -.
DR PRO; PR:Q9SZT4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9SZT4; baseline and differential.
DR Genevisible; Q9SZT4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005227; F:calcium activated cation channel activity; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IMP:TAIR.
DR InterPro; IPR045122; Csc1-like.
DR InterPro; IPR032880; Csc1_N.
DR InterPro; IPR027815; PHM7_cyt.
DR InterPro; IPR003864; RSN1_7TM.
DR PANTHER; PTHR13018; PTHR13018; 1.
DR Pfam; PF14703; PHM7_cyt; 1.
DR Pfam; PF02714; RSN1_7TM; 2.
DR Pfam; PF13967; RSN1_TM; 1.
PE 2: Evidence at transcript level;
KW Calcium; Ion channel; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..817
FT /note="CSC1-like protein At4g35870"
FT /id="PRO_0000429812"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 569..589
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 676..696
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 729..749
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 817 AA; 92585 MW; 1BE1DE4956F2145E CRC64;
MNRNFSPAAM PPISSMTIDN SFSPPPSSGD LPEIPDAWYG NIQYLLNISV IGLLCCVSIF
LFVKLRSDHR RMPGPSALFS KLLAVWKATC REIARHCGAD AAQFLLIEGG SFVLLFSIAV
LAVSVMLPLN LYAGTALLSD ELSKTMITHI QKGSALLWLH FVFVVIVVVI SHFGIAAIEA
RLKFTRFRDG NGNISDPNAN STAVFTIMVQ GLPKNLGSDR VEFEDCFRLK YPGKVYKFIV
PMDLCALDDL ATELVRVRDE ITWLVAKMDS RLLPDEYENV GDNGLVFCVC SLWVRVKVLW
SQITERFGFT DDEKLRKLQE LRADLESQLA AYKEGRAQGA GVAFVMFKDV YTANKAVQDF
RNERSRRTGK FFSVTELRLQ RNQWKVDRAP LATDIYWNHL GLTKVALIVR RVIVNTILLL
ILVFFSSPLA LISALVSAGR IFNAEALDSA QYWLTWVQTS GWIGSLIFQF LPNVFIFVSM
YIVIPSALSY LSKFERHLTV SGEQRAALLK MVCFFLVNLI ILKALVESSL ESALLKMSRC
YLDGEDCKRI EEYMSPSFLS RSCVSALAFL ITSTFLGISF DLLAPIPWIK KKIQKFRKND
MLQLVPEQNE EYALENQEPS SNLETPLLPE NMFESPRFGD IEPMSQDLSE YPISRTSPIP
KQKFDFAQYY AFNLTIFALT MIYSSFAPLV VPVGAVYFGY RYIVDKYNFL YVYRVRGFPA
GNEGKLMDTV LCIMRFCVDL YLVSMLLFFS VKGDSTKLQA IFTLGVLVMY KLLPSDTDRY
HPALLRSIQT VDSIIDGPVD YEAYSHPNFD WDTYNNR
