ID   CSD_HALSA               Reviewed;         415 AA.
AC   Q9HMM6;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Probable cysteine desulfurase;
DE            EC=2.8.1.7;
GN   Name=csd; OrderedLocusNames=VNG_2471G;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-
CC         alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA-
CC         COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972,
CC         ChEBI:CHEBI:64428; EC=2.8.1.7;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. Csd subfamily. {ECO:0000305}.
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DR   EMBL; AE004437; AAG20545.1; -; Genomic_DNA.
DR   PIR; E84397; E84397.
DR   RefSeq; WP_010903847.1; NC_002607.1.
DR   AlphaFoldDB; Q9HMM6; -.
DR   SMR; Q9HMM6; -.
DR   STRING; 64091.VNG_2471G; -.
DR   PaxDb; Q9HMM6; -.
DR   EnsemblBacteria; AAG20545; AAG20545; VNG_2471G.
DR   GeneID; 5953398; -.
DR   GeneID; 62885598; -.
DR   KEGG; hal:VNG_2471G; -.
DR   PATRIC; fig|64091.14.peg.1913; -.
DR   HOGENOM; CLU_003433_2_5_2; -.
DR   InParanoid; Q9HMM6; -.
DR   OMA; HKLCGPT; -.
DR   OrthoDB; 24071at2157; -.
DR   PhylomeDB; Q9HMM6; -.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0031071; F:cysteine desulfurase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0006534; P:cysteine metabolic process; IEA:InterPro.
DR   CDD; cd06453; SufS_like; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR010970; Cys_dSase_SufS.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   TIGRFAMs; TIGR01979; sufS; 1.
PE   3: Inferred from homology;
KW   Pyridoxal phosphate; Reference proteome; Transferase.
FT   CHAIN           1..415
FT                   /note="Probable cysteine desulfurase"
FT                   /id="PRO_0000150326"
FT   ACT_SITE        372
FT                   /note="Cysteine persulfide intermediate"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         233
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   415 AA;  45113 MW;  49BAFCA5169B5F37 CRC64;
     MEATEQGPID VEAVRADFPI LEREVAGGED LVYLDNAATS HTPEPVVDAI ADYYRRYNSN
     VHRGLHELSQ EASVAYEDAH DKLAAFVGGE DREEMVFTKN TTEAENLVAF AWGLNELGPG
     DEVVLTQMEH HASLVTWQQV ADETGAEVKY IPITDDGHLD MDAAADMITD DTALVNAVHI
     SNTLGTVNPV GELADIAHDH GAYIFVDGAQ AAPTRAVDVQ EIDADFYAFS GHKMLGPTGI
     GCLYGKRHLL AEMEPFLYGG DMIERVSYED ATWNDPPWKF EAGTPVIAQG IALAEAVDYL
     QDIGMDAIRA HEEALTEYAY DQLTMTDDVD VYGPPGDDRG AVVSFNVDGI HAHDLSSILN
     DYGVAIRAGD HCTQPLHDTL GVPASARASF YLYNTRDEVD ALVAAVDEAR QIFAP