ID   CSD_PSEAE               Reviewed;         401 AA.
AC   Q9HXX3;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Probable cysteine desulfurase;
DE            EC=2.8.1.7;
GN   Name=csd; OrderedLocusNames=PA3667;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the removal of elemental sulfur and selenium atoms
CC       from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to
CC       produce L-alanine. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-
CC         alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA-
CC         COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972,
CC         ChEBI:CHEBI:64428; EC=2.8.1.7;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. Csd subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004091; AAG07055.1; -; Genomic_DNA.
DR   PIR; F83187; F83187.
DR   RefSeq; NP_252357.1; NC_002516.2.
DR   RefSeq; WP_003098634.1; NZ_QZGE01000001.1.
DR   AlphaFoldDB; Q9HXX3; -.
DR   SMR; Q9HXX3; -.
DR   STRING; 287.DR97_4210; -.
DR   PaxDb; Q9HXX3; -.
DR   PRIDE; Q9HXX3; -.
DR   EnsemblBacteria; AAG07055; AAG07055; PA3667.
DR   GeneID; 880538; -.
DR   KEGG; pae:PA3667; -.
DR   PATRIC; fig|208964.12.peg.3836; -.
DR   PseudoCAP; PA3667; -.
DR   HOGENOM; CLU_003433_2_5_6; -.
DR   InParanoid; Q9HXX3; -.
DR   OMA; PVCLRYG; -.
DR   PhylomeDB; Q9HXX3; -.
DR   BioCyc; PAER208964:G1FZ6-3737-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0031071; F:cysteine desulfurase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR   GO; GO:0006534; P:cysteine metabolic process; IEA:InterPro.
DR   CDD; cd06453; SufS_like; 1.
DR   Gene3D; 3.40.640.10; -; 1.
DR   Gene3D; 3.90.1150.10; -; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR020578; Aminotrans_V_PyrdxlP_BS.
DR   InterPro; IPR010970; Cys_dSase_SufS.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
DR   PROSITE; PS00595; AA_TRANSFER_CLASS_5; 1.
PE   3: Inferred from homology;
KW   Pyridoxal phosphate; Reference proteome; Transferase.
FT   CHAIN           1..401
FT                   /note="Probable cysteine desulfurase"
FT                   /id="PRO_0000150307"
FT   MOD_RES         223
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   401 AA;  43274 MW;  AB944EFD7E64699B CRC64;
     MSLPSPWRAD FPAFSAFAAE GLTYLDSAAT AQKPQAVLDA LNGYYLGGAA NVHRAQHVPG
     ERATRAFEAA RSRVAHWLHA GNPAEVLFTR GTTEAINLVA YGLERHFRPG DELLVSALEH
     HANLLPWQQL ALRRGLVLRV LPLDERGVID LEQARHIIGE RTRLLAISQL SNVLGTWQPV
     VELIQLARER GAWTLVDGAQ GSVHGRHDLP GLGCDFYAFS GHKLYGPDGI GVLWGRPQAL
     EQLAHWQFGG EMVRHTGFHE ASFHAAPLGF EAGTPAVSAA IGLGATIDWL ATLDEAEVAA
     HEGALHARLL AGLLARDGVS LLGEPQAALA SFCVDGVHVA DLAHLLGEQG IAVRAGHHCA
     MPLLQRLEVP GALRVSLGLY NDADDLERFF LALDRSLELL R