ACPT_ECOLI
ID ACPT_ECOLI Reviewed; 195 AA.
AC P37623; Q2M7D8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=4'-phosphopantetheinyl transferase AcpT;
DE EC=2.7.8.7 {ECO:0000305|PubMed:10625633};
GN Name=acpT {ECO:0000303|PubMed:10625633}; Synonyms=yhhU;
GN OrderedLocusNames=b3475, JW3440;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RX PubMed=8939709; DOI=10.1016/s1074-5521(96)90181-7;
RA Lambalot R.H., Gehring A.M., Flugel R.S., Zuber P., LaCelle M.,
RA Marahiel M.A., Reid R., Khosla C., Walsh C.T.;
RT "A new enzyme superfamily -- the phosphopantetheinyl transferases.";
RL Chem. Biol. 3:923-936(1996).
RN [5]
RP FUNCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=10625633; DOI=10.1074/jbc.275.2.959;
RA Flugel R.S., Hwangbo Y., Lambalot R.H., Cronan J.E. Jr., Walsh C.T.;
RT "Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in
RT Escherichia coli.";
RL J. Biol. Chem. 275:959-968(2000).
CC -!- FUNCTION: May be involved in an alternative pathway for
CC phosphopantetheinyl transfer and holo-ACP synthesis in E.coli. The
CC native apo-protein substrate is unknown. Is able to functionally
CC replace AcpS in vivo but only when expressed at high levels.
CC {ECO:0000269|PubMed:10625633, ECO:0000269|PubMed:8939709}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-
CC [ACP]; Xref=Rhea:RHEA:12068, Rhea:RHEA-COMP:9685, Rhea:RHEA-
CC COMP:9690, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58343, ChEBI:CHEBI:64479; EC=2.7.8.7;
CC Evidence={ECO:0000305|PubMed:10625633};
CC -!- SIMILARITY: Belongs to the P-Pant transferase superfamily.
CC Gsp/Sfp/HetI/AcpT family. {ECO:0000305}.
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DR EMBL; U00039; AAB18450.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76500.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77818.1; -; Genomic_DNA.
DR PIR; S47694; S47694.
DR RefSeq; NP_417932.1; NC_000913.3.
DR RefSeq; WP_000285774.1; NZ_SSZK01000008.1.
DR AlphaFoldDB; P37623; -.
DR SMR; P37623; -.
DR BioGRID; 4259300; 100.
DR DIP; DIP-12355N; -.
DR IntAct; P37623; 1.
DR STRING; 511145.b3475; -.
DR jPOST; P37623; -.
DR PaxDb; P37623; -.
DR PRIDE; P37623; -.
DR EnsemblBacteria; AAC76500; AAC76500; b3475.
DR EnsemblBacteria; BAE77818; BAE77818; BAE77818.
DR GeneID; 947979; -.
DR KEGG; ecj:JW3440; -.
DR KEGG; eco:b3475; -.
DR PATRIC; fig|1411691.4.peg.3250; -.
DR EchoBASE; EB2135; -.
DR eggNOG; COG2091; Bacteria.
DR HOGENOM; CLU_119926_0_0_6; -.
DR InParanoid; P37623; -.
DR OMA; WQIVSID; -.
DR PhylomeDB; P37623; -.
DR BioCyc; EcoCyc:EG12221-MON; -.
DR BioCyc; MetaCyc:EG12221-MON; -.
DR PRO; PR:P37623; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008897; F:holo-[acyl-carrier-protein] synthase activity; IDA:EcoliWiki.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IGI:EcoliWiki.
DR GO; GO:0019878; P:lysine biosynthetic process via aminoadipic acid; IBA:GO_Central.
DR GO; GO:0018070; P:peptidyl-serine phosphopantetheinylation; NAS:EcoliWiki.
DR Gene3D; 3.90.470.20; -; 1.
DR InterPro; IPR008278; 4-PPantetheinyl_Trfase_dom.
DR InterPro; IPR037143; 4-PPantetheinyl_Trfase_dom_sf.
DR Pfam; PF01648; ACPS; 1.
DR SUPFAM; SSF56214; SSF56214; 2.
PE 3: Inferred from homology;
KW Reference proteome; Transferase.
FT CHAIN 1..195
FT /note="4'-phosphopantetheinyl transferase AcpT"
FT /id="PRO_0000206081"
SQ SEQUENCE 195 AA; 21768 MW; 29385FDA343B2AB7 CRC64;
MYRIVLGKVS TLSAAPLPPG LREQAPQGPR RERWLAGRAL LSHTLSPLPE IIYGEQGKPA
FAPEMPLWFN LSHSGDDIAL LLSDEGEVGC DIEVIRPRAN WRWLANAVFS LGEHAEMDAV
HPDQQLEMFW RIWTRKEAIV KQRGGSAWQI VSVDSTYHSS LSVSHCQLEN LSLAICTPTP
FTLTADSVQW IDSVN
