ID   CSE2_THET8              Reviewed;         169 AA.
AC   Q53VY0;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=CRISPR-associated protein Cse2;
DE   AltName: Full=CRISPR type I-E/ECOLI-associated protein CasB/Cse2;
GN   Name=cse2; OrderedLocusNames=TTHB189;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OG   Plasmid pTT27.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS).
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RX   PubMed=18798563; DOI=10.1002/prot.22224;
RA   Agari Y., Yokoyama S., Kuramitsu S., Shinkai A.;
RT   "X-ray crystal structure of a CRISPR-associated protein, Cse2, from Thermus
RT   thermophilus HB8.";
RL   Proteins 73:1063-1067(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated CasB/Cse2 family.
CC       {ECO:0000305}.
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DR   EMBL; AP008227; BAD71985.1; -; Genomic_DNA.
DR   RefSeq; WP_011229115.1; NC_006462.1.
DR   RefSeq; YP_145428.1; NC_006462.1.
DR   PDB; 2ZCA; X-ray; 1.80 A; A/B=1-169.
DR   PDB; 4H7A; X-ray; 2.60 A; A/B=1-156.
DR   PDBsum; 2ZCA; -.
DR   PDBsum; 4H7A; -.
DR   AlphaFoldDB; Q53VY0; -.
DR   SMR; Q53VY0; -.
DR   MINT; Q53VY0; -.
DR   EnsemblBacteria; BAD71985; BAD71985; BAD71985.
DR   GeneID; 3167892; -.
DR   KEGG; ttj:TTHB189; -.
DR   PATRIC; fig|300852.9.peg.2140; -.
DR   HOGENOM; CLU_081588_3_0_0; -.
DR   OMA; WARAFYR; -.
DR   EvolutionaryTrace; Q53VY0; -.
DR   Proteomes; UP000000532; Plasmid pTT27.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   CDD; cd09670; Cse2_I-E; 1.
DR   Gene3D; 1.10.520.40; -; 1.
DR   InterPro; IPR013382; CRISPR-assoc_prot_Cse2.
DR   InterPro; IPR038287; Cse2_sf.
DR   Pfam; PF09485; CRISPR_Cse2; 1.
DR   TIGRFAMs; TIGR02548; casB_cse2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antiviral defense; Plasmid; Reference proteome.
FT   CHAIN           1..169
FT                   /note="CRISPR-associated protein Cse2"
FT                   /id="PRO_0000418433"
FT   HELIX           3..15
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           20..29
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           30..32
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           39..41
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           42..49
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           56..71
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   TURN            72..74
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           82..90
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           96..104
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   TURN            108..110
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           111..121
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           128..136
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   TURN            137..139
FT                   /evidence="ECO:0007829|PDB:2ZCA"
FT   HELIX           144..155
FT                   /evidence="ECO:0007829|PDB:2ZCA"
SQ   SEQUENCE   169 AA;  19405 MW;  FC9C4C109071B71B CRC64;
     MSPGERFLDW LKRLQGQKAW TAARAAFRRS LAFPPGAYPR AMPYVEPFLA KGDWRQEERE
     AHYLVAALYA LKDGDHQVGR TLARALWEKA QGSASVEKRF LALLEADRDQ IAFRLRQAVA
     LVEGGIDFAR LLDDLLRWFS PERHVQARWA REYYGAGASE EEKKKEVEA