CSEPA_MELLP
ID CSEPA_MELLP Reviewed; 96 AA.
AC F4RBY2;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 23.
DE RecName: Full=Candidate secreted effector protein MPL124478 {ECO:0000303|PubMed:21644839};
DE Short=CSEP MPL124478 {ECO:0000303|PubMed:21644839};
DE AltName: Full=Small secreted protein MPL124478 {ECO:0000303|PubMed:21536894};
DE Short=SSP MPL124478 {ECO:0000303|PubMed:21536894};
DE Flags: Precursor;
GN ORFNames=MELLADRAFT_124478, MPL124478;
OS Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) (Poplar leaf
OS rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Melampsoraceae; Melampsora.
OX NCBI_TaxID=747676;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION.
RC STRAIN=98AG31 / pathotype 3-4-7;
RX PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA Grigoriev I.V., Szabo L.J., Martin F.;
RT "Obligate biotrophy features unraveled by the genomic analysis of rust
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
RN [2]
RP INDUCTION.
RX PubMed=21644839; DOI=10.1094/mpmi-01-11-0006;
RA Duplessis S., Hacquard S., Delaruelle C., Tisserant E., Frey P., Martin F.,
RA Kohler A.;
RT "Melampsora larici-populina transcript profiling during germination and
RT timecourse infection of poplar leaves reveals dynamic expression patterns
RT associated with virulence and biotrophy.";
RL Mol. Plant Microbe Interact. 24:808-818(2011).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=25650830; DOI=10.1094/mpmi-01-15-0003-r;
RA Petre B., Saunders D.G., Sklenar J., Lorrain C., Win J., Duplessis S.,
RA Kamoun S.;
RT "Candidate effector proteins of the rust pathogen Melampsora larici-
RT populina target diverse plant cell compartments.";
RL Mol. Plant Microbe Interact. 28:689-700(2015).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF 29-ARG--LYS-38.
RX PubMed=30283062; DOI=10.1038/s41598-018-32825-0;
RA Ahmed M.B., Santos K.C.G.D., Sanchez I.B., Petre B., Lorrain C.,
RA Plourde M.B., Duplessis S., Desgagne-Penix I., Germain H.;
RT "A rust fungal effector binds plant DNA and modulates transcription.";
RL Sci. Rep. 8:14718-14718(2018).
CC -!- FUNCTION: Rust effector delivered into infected tissues to modulate
CC host functions and contribute to pathogen virulence (PubMed:30283062).
CC Binds the TGA1a-binding DNA sequence to repress the expression of genes
CC involved in immune responses such as the defense-related transcription
CC factors WRKY18, WRKY27, WRKY33, MYB51, the defense-related proteins
CC NHL3, RPP8, YLS9, AZI1, CRK11 and the jasmonate pathway and regulation
CC genes JAZ1, ASA1, ASB1. Other genes involved in diverse mechanisms also
CC down-regulated include the chitinase CHI, the brassinosteroid-related
CC genes BAS1, BES1 PAR1, BEE1, the salicylic acid-related gene NPR3, the
CC ethylene-related response genes ARGOS and ARGOS-like (ARL), EBF2, ERF6,
CC ETR2, RTE1, the carbon metabolism-related gene EXO, as well as the
CC red/far red light signalization-related genes FAR1, GA2OX2, PAR1, PIF3,
CC PKS4 (PubMed:30283062). {ECO:0000269|PubMed:30283062}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25650830,
CC ECO:0000269|PubMed:30283062}. Host nucleus
CC {ECO:0000269|PubMed:25650830, ECO:0000269|PubMed:30283062}. Host
CC nucleus, host nucleolus {ECO:0000269|PubMed:25650830,
CC ECO:0000269|PubMed:30283062}.
CC -!- INDUCTION: Expressed during host infection.
CC {ECO:0000269|PubMed:21644839}.
CC -!- DOMAIN: The nucleolar localization signal (residues 29 to 38) is
CC required for host nucleolar accumulation but not for the nuclear
CC localization, nor for the augmented plant susceptibility to pathogens.
CC {ECO:0000269|PubMed:30283062}.
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DR EMBL; GL883095; EGG10259.1; -; Genomic_DNA.
DR RefSeq; XP_007406560.1; XM_007406498.1.
DR AlphaFoldDB; F4RBY2; -.
DR EnsemblFungi; EGG10259; EGG10259; MELLADRAFT_124478.
DR GeneID; 18926773; -.
DR KEGG; mlr:MELLADRAFT_124478; -.
DR VEuPathDB; FungiDB:MELLADRAFT_124478; -.
DR HOGENOM; CLU_183918_0_0_1; -.
DR PHI-base; PHI:8555; -.
DR Proteomes; UP000001072; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Host nucleus; Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..96
FT /note="Candidate secreted effector protein MPL124478"
FT /id="PRO_5003320712"
FT REGION 27..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 29..36
FT /note="Nucleolar localization signal"
FT /evidence="ECO:0000269|PubMed:30283062"
FT MUTAGEN 29..38
FT /note="Missing: Impairs the translocation to he host
FT nucleolus but still localizes to the nucleoplasm."
FT /evidence="ECO:0000269|PubMed:30283062"
SQ SEQUENCE 96 AA; 9731 MW; 636AAFCCF9DB06A9 CRC64;
MMSQRYFKLL VVFLGLFAMI ASIAEGKGRH KNGGGSRKKT NTVNNGGNNG GKPKVPMCGT
TAATCSKGSP SCKGGKPTCG SKGAFNACGG NVKVTC
