ID   CSEPC_MELLP             Reviewed;          72 AA.
AC   F4RC55;
DT   08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Candidate secreted effector protein MPL124499 {ECO:0000303|PubMed:22046958};
DE            Short=CSEP MPL124499 {ECO:0000303|PubMed:22046958};
DE   AltName: Full=Small secreted protein MPL124499 {ECO:0000303|PubMed:21536894};
DE            Short=SSP MPL124499 {ECO:0000303|PubMed:21536894};
DE   Flags: Precursor;
GN   ORFNames=MELLADRAFT_124499, MPL124499;
OS   Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) (Poplar leaf
OS   rust fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC   Pucciniomycetes; Pucciniales; Melampsoraceae; Melampsora.
OX   NCBI_TaxID=747676;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION.
RC   STRAIN=98AG31 / pathotype 3-4-7;
RX   PubMed=21536894; DOI=10.1073/pnas.1019315108;
RA   Duplessis S., Cuomo C.A., Lin Y.-C., Aerts A., Tisserant E.,
RA   Veneault-Fourrey C., Joly D.L., Hacquard S., Amselem J., Cantarel B.L.,
RA   Chiu R., Coutinho P.M., Feau N., Field M., Frey P., Gelhaye E.,
RA   Goldberg J., Grabherr M.G., Kodira C.D., Kohler A., Kuees U.,
RA   Lindquist E.A., Lucas S.M., Mago R., Mauceli E., Morin E., Murat C.,
RA   Pangilinan J.L., Park R., Pearson M., Quesneville H., Rouhier N.,
RA   Sakthikumar S., Salamov A.A., Schmutz J., Selles B., Shapiro H.,
RA   Tanguay P., Tuskan G.A., Henrissat B., Van de Peer Y., Rouze P.,
RA   Ellis J.G., Dodds P.N., Schein J.E., Zhong S., Hamelin R.C.,
RA   Grigoriev I.V., Szabo L.J., Martin F.;
RT   "Obligate biotrophy features unraveled by the genomic analysis of rust
RT   fungi.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:9166-9171(2011).
RN   [2]
RP   INDUCTION.
RX   PubMed=20615251; DOI=10.1186/1471-2164-11-422;
RA   Joly D.L., Feau N., Tanguay P., Hamelin R.C.;
RT   "Comparative analysis of secreted protein evolution using expressed
RT   sequence tags from four poplar leaf rusts (Melampsora spp.).";
RL   BMC Genomics 11:422-422(2010).
RN   [3]
RP   INDUCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=22046958; DOI=10.1094/mpmi-09-11-0238;
RA   Hacquard S., Joly D.L., Lin Y.C., Tisserant E., Feau N., Delaruelle C.,
RA   Legue V., Kohler A., Tanguay P., Petre B., Frey P., Van de Peer Y.,
RA   Rouze P., Martin F., Hamelin R.C., Duplessis S.;
RT   "A comprehensive analysis of genes encoding small secreted proteins
RT   identifies candidate effectors in Melampsora larici-populina (poplar leaf
RT   rust).";
RL   Mol. Plant Microbe Interact. 25:279-293(2012).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=27868319; DOI=10.1111/mpp.12514;
RA   Germain H., Joly D.L., Mireault C., Plourde M.B., Letanneur C., Stewart D.,
RA   Morency M.J., Petre B., Duplessis S., Seguin A.;
RT   "Infection assays in Arabidopsis reveal candidate effectors from the poplar
RT   rust fungus that promote susceptibility to bacteria and oomycete
RT   pathogens.";
RL   Mol. Plant Pathol. 19:191-200(2018).
CC   -!- FUNCTION: Rust effector delivered into infected tissues to modulate
CC       host functions and contribute to pathogen virulence (PubMed:27868319).
CC       Enhances leaf colonization by the bacteria Pseudomonas syringae and the
CC       oomycete Hyaloperonospora arabidopsidis pathogens in an Arabidopsis
CC       thaliana infection model (PubMed:27868319).
CC       {ECO:0000269|PubMed:27868319}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22046958}. Host cell
CC       {ECO:0000269|PubMed:22046958}. Host cytoplasm
CC       {ECO:0000269|PubMed:27868319}. Host nucleus
CC       {ECO:0000269|PubMed:27868319}.
CC   -!- INDUCTION: Expression is induced during host infection.
CC       {ECO:0000269|PubMed:20615251, ECO:0000269|PubMed:22046958}.
CC   -!- SIMILARITY: Belongs to the CPGH1 family. {ECO:0000305}.
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DR   EMBL; GL883095; EGG10221.1; -; Genomic_DNA.
DR   RefSeq; XP_007406522.1; XM_007406460.1.
DR   AlphaFoldDB; F4RC55; -.
DR   EnsemblFungi; EGG10221; EGG10221; MELLADRAFT_124499.
DR   GeneID; 18926786; -.
DR   KEGG; mlr:MELLADRAFT_124499; -.
DR   VEuPathDB; FungiDB:MELLADRAFT_124499; -.
DR   HOGENOM; CLU_198490_1_0_1; -.
DR   Proteomes; UP000001072; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Host nucleus; Reference proteome; Secreted; Signal;
KW   Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..72
FT                   /note="Candidate secreted effector protein MPL124499"
FT                   /id="PRO_5003320713"
SQ   SEQUENCE   72 AA;  7747 MW;  DD572BBD2A07DA73 CRC64;
     MKLSIFAAIF MAFVSLNQVF GSMTHSVKSK EAIISADESL VKPIDAGQAQ PEGCPVCARL
     GVLCPYHYRA DS