ID   3001L_ASFB7             Reviewed;         268 AA.
AC   Q89705;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   11-NOV-2015, sequence version 2.
DT   23-FEB-2022, entry version 62.
DE   RecName: Full=Protein MGF 300-1L;
GN   OrderedLocusNames=BA71V-019; ORFNames=J268L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9049318; DOI=10.1099/0022-1317-76-4-729;
RA   Almazan F., Murguia J.R., Rodriguez J.M., de la Vega I., Vinuela E.;
RT   "A set of African swine fever virus tandem repeats shares similarities with
RT   SAR-like sequences.";
RL   J. Gen. Virol. 76:729-740(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [3]
RP   SEQUENCE REVISION.
RA   Rodriguez J.M., Salas M.L.;
RL   Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
CC   -!- FUNCTION: Plays a role in virus cell tropism, and may be required for
CC       efficient virus replication in macrophages. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- SIMILARITY: Belongs to the asfivirus MGF 300 family. {ECO:0000305}.
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DR   EMBL; U13763; AAC54518.1; -; Genomic_DNA.
DR   EMBL; U18466; AAA65250.2; -; Genomic_DNA.
DR   RefSeq; NP_042714.2; NC_001659.2.
DR   GeneID; 22220404; -.
DR   KEGG; vg:22220404; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Early protein; Glycoprotein; Host membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..268
FT                   /note="Protein MGF 300-1L"
FT                   /id="PRO_0000373226"
FT   TOPO_DOM        1..175
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        194..268
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        227
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        117
FT                   /note="R -> C (in Ref. 1; AAC54518)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        120
FT                   /note="M -> K (in Ref. 1; AAC54518)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   268 AA;  31396 MW;  62298E9E51A57959 CRC64;
     MVSLTTCCLK NIVNQHACVE NTVLLYHLGL RWNCKTLYQC TQCNGVNYTN SHSDQCKNKD
     LFLMKVIVKK NLAVTRTLLS WGASPEYARL FCRNTEEEQA LNVQHVADVS SSKILERLTM
     SYKENDEQLL ITFYLLNLST KFSTNLREQV RFNIVSYIIC DLAIHQTFKT FYAKNYSLST
     LYCIFLAIYY KLYTALRKMV KIYPGLKRFA YLIGFMFDDE TVMETYNSTD DEISECKNRI
     IAIKGYYGNI HCRSDIDHMY AFSQNDYW