CSF2_MOUSE
ID CSF2_MOUSE Reviewed; 141 AA.
AC P01587;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Granulocyte-macrophage colony-stimulating factor;
DE Short=GM-CSF;
DE AltName: Full=Colony-stimulating factor;
DE Short=CSF;
DE Flags: Precursor;
GN Name=Csf2; Synonyms=Csfgm;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3876930; DOI=10.1002/j.1460-2075.1985.tb03971.x;
RA Miyatake S., Otsuka T., Yokota T., Lee F., Arai K.;
RT "Structure of the chromosomal gene for granulocyte-macrophage colony
RT stimulating factor: comparison of the mouse and human genes.";
RL EMBO J. 4:2561-2568(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3876931; DOI=10.1002/j.1460-2075.1985.tb03972.x;
RA Stanley E.R., Metcalf D., Sobieszczuk P., Gough N.M., Dunn A.R.;
RT "The structure and expression of the murine gene encoding granulocyte-
RT macrophage colony stimulating factor: evidence for utilisation of
RT alternative promoters.";
RL EMBO J. 4:2569-2573(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3902470; DOI=10.1002/j.1460-2075.1985.tb03973.x;
RA Delamarter J.F., Mermod J.-J., Liang C.M., Eliason J.F., Thatcher D.R.;
RT "Recombinant murine GM-CSF from E. coli has biological activity and is
RT neutralized by a specific antiserum.";
RL EMBO J. 4:2575-2581(1985).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RX PubMed=3874057; DOI=10.1002/j.1460-2075.1985.tb03678.x;
RA Gough N.M., Metcalf D., Gough J., Grail D., Dunn A.R.;
RT "Structure and expression of the mRNA for murine granulocyte-macrophage
RT colony stimulating factor.";
RL EMBO J. 4:645-653(1985).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 24-141.
RC TISSUE=Lung;
RX PubMed=6610831; DOI=10.1038/309763a0;
RA Gough N.M., Gough J., Metcalf D., Kelso A., Grail D., Nicola N.A.,
RA Burgess A.W., Dunn A.R.;
RT "Molecular cloning of cDNA encoding a murine haematopoietic growth
RT regulator, granulocyte-macrophage colony stimulating factor.";
RL Nature 309:763-767(1984).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 18-141.
RX PubMed=3898082; DOI=10.1073/pnas.82.18.6250;
RA Cantrell M.A., Anderson D., Cerretti D.P., Price V., McKereghan K.,
RA Tushinski R.J., Mochizuki D.Y., Larsen A., Grabstein S., Gillis S.,
RA Cosman D.;
RT "Cloning, sequence, and expression of a human granulocyte/macrophage
RT colony-stimulating factor.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:6250-6254(1985).
RN [7]
RP PROTEIN SEQUENCE OF 24-57.
RX PubMed=3871523; DOI=10.1073/pnas.82.2.292;
RA Sparrow L.G., Metcalf D., Hunkapiller M.W., Hood L.E., Burgess A.W.;
RT "Purification and partial amino acid sequence of asialo murine granulocyte-
RT macrophage colony stimulating factor.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:292-296(1985).
RN [8]
RP DISULFIDE BONDS.
RX PubMed=3318813; DOI=10.1042/bj2470195;
RA Schrimser J.L., Rose K., Simona M.G., Wingfield P.;
RT "Characterization of human and mouse granulocyte-macrophage-colony-
RT stimulating factors derived from Escherichia coli.";
RL Biochem. J. 247:195-199(1987).
RN [9]
RP MUTAGENESIS.
RX PubMed=2002066; DOI=10.1016/s0021-9258(19)67792-6;
RA Altmann S.W., Johnson G.D., Prystowsky M.B.;
RT "Single proline substitutions in predicted alpha-helices of murine
RT granulocyte-macrophage colony-stimulating factor result in a loss in
RT bioactivity and altered glycosylation.";
RL J. Biol. Chem. 266:5333-5341(1991).
CC -!- FUNCTION: Cytokine that stimulates the growth and differentiation of
CC hematopoietic precursor cells from various lineages, including
CC granulocytes, macrophages, eosinophils and erythrocytes.
CC -!- SUBUNIT: Monomer. The signaling GM-CSF receptor complex is a dodecamer
CC of two head-to-head hexamers of two alpha, two beta, and two ligand
CC subunits (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the GM-CSF family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA26192.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X03020; CAA26821.1; -; Genomic_DNA.
DR EMBL; X03019; CAA26820.1; -; mRNA.
DR EMBL; X02333; CAA26193.1; -; mRNA.
DR EMBL; X02333; CAA26192.1; ALT_INIT; mRNA.
DR EMBL; X05906; CAA29336.1; -; mRNA.
DR EMBL; M11848; AAA37483.1; -; mRNA.
DR CCDS; CCDS24692.1; -.
DR PIR; I48368; FQMSGM.
DR RefSeq; NP_034099.2; NM_009969.4.
DR AlphaFoldDB; P01587; -.
DR SMR; P01587; -.
DR STRING; 10090.ENSMUSP00000019060; -.
DR GlyGen; P01587; 4 sites.
DR PhosphoSitePlus; P01587; -.
DR PaxDb; P01587; -.
DR PRIDE; P01587; -.
DR Antibodypedia; 14363; 1838 antibodies from 49 providers.
DR DNASU; 12981; -.
DR Ensembl; ENSMUST00000019060; ENSMUSP00000019060; ENSMUSG00000018916.
DR GeneID; 12981; -.
DR KEGG; mmu:12981; -.
DR UCSC; uc007ixm.1; mouse.
DR CTD; 1437; -.
DR MGI; MGI:1339752; Csf2.
DR VEuPathDB; HostDB:ENSMUSG00000018916; -.
DR eggNOG; ENOG502TDUI; Eukaryota.
DR GeneTree; ENSGT00390000013425; -.
DR HOGENOM; CLU_152286_0_0_1; -.
DR InParanoid; P01587; -.
DR OMA; VTRPWKH; -.
DR PhylomeDB; P01587; -.
DR TreeFam; TF338611; -.
DR Reactome; R-MMU-512988; Interleukin-3, Interleukin-5 and GM-CSF signaling.
DR Reactome; R-MMU-5673001; RAF/MAP kinase cascade.
DR Reactome; R-MMU-912526; Interleukin receptor SHC signaling.
DR BioGRID-ORCS; 12981; 1 hit in 76 CRISPR screens.
DR ChiTaRS; Csf2; mouse.
DR PRO; PR:P01587; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; P01587; protein.
DR Bgee; ENSMUSG00000018916; Expressed in left lung lobe and 20 other tissues.
DR ExpressionAtlas; P01587; baseline and differential.
DR Genevisible; P01587; MM.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0030526; C:granulocyte macrophage colony-stimulating factor receptor complex; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005125; F:cytokine activity; IDA:MGI.
DR GO; GO:0005129; F:granulocyte macrophage colony-stimulating factor receptor binding; IEA:InterPro.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0008283; P:cell population proliferation; IDA:MGI.
DR GO; GO:0097011; P:cellular response to granulocyte macrophage colony-stimulating factor stimulus; ISO:MGI.
DR GO; GO:0097028; P:dendritic cell differentiation; ISO:MGI.
DR GO; GO:0001892; P:embryonic placenta development; IMP:MGI.
DR GO; GO:0042045; P:epithelial fluid transport; ISO:MGI.
DR GO; GO:0038157; P:granulocyte-macrophage colony-stimulating factor signaling pathway; ISO:MGI.
DR GO; GO:0001821; P:histamine secretion; ISO:MGI.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0042116; P:macrophage activation; ISO:MGI.
DR GO; GO:0030225; P:macrophage differentiation; ISO:MGI.
DR GO; GO:0030224; P:monocyte differentiation; IDA:MGI.
DR GO; GO:0030099; P:myeloid cell differentiation; IBA:GO_Central.
DR GO; GO:0043011; P:myeloid dendritic cell differentiation; IDA:MGI.
DR GO; GO:2001240; P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0030223; P:neutrophil differentiation; IDA:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:MGI.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR GO; GO:0032747; P:positive regulation of interleukin-23 production; ISO:MGI.
DR GO; GO:0070665; P:positive regulation of leukocyte proliferation; ISO:MGI.
DR GO; GO:0010744; P:positive regulation of macrophage derived foam cell differentiation; ISO:MGI.
DR GO; GO:0071803; P:positive regulation of podosome assembly; ISO:MGI.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:MGI.
DR GO; GO:0006468; P:protein phosphorylation; ISO:MGI.
DR GO; GO:0007259; P:receptor signaling pathway via JAK-STAT; ISO:MGI.
DR GO; GO:0042127; P:regulation of cell population proliferation; IGI:MGI.
DR GO; GO:0045187; P:regulation of circadian sleep/wake cycle, sleep; ISO:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IDA:MGI.
DR CDD; cd00040; CSF2; 1.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR000773; GM_colony-stim-fac.
DR PANTHER; PTHR10059; PTHR10059; 1.
DR Pfam; PF01109; GM_CSF; 1.
DR PRINTS; PR00693; GMCSFACTOR.
DR SMART; SM00040; CSF2; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00702; GM_CSF; 1.
PE 1: Evidence at protein level;
KW Cytokine; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Growth factor; Reference proteome; Secreted; Signal.
FT SIGNAL 1..17
FT CHAIN 18..141
FT /note="Granulocyte-macrophage colony-stimulating factor"
FT /id="PRO_0000005866"
FT CARBOHYD 22
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000250"
FT CARBOHYD 27
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) asparagine"
FT DISULFID 68..110
FT /evidence="ECO:0000269|PubMed:3318813"
FT DISULFID 102..135
FT /evidence="ECO:0000269|PubMed:3318813"
FT MUTAGEN 38
FT /note="E->P: Reduction in bioactivity."
FT /evidence="ECO:0000269|PubMed:2002066"
FT MUTAGEN 73
FT /note="L->P: 25-fold reduction in bioactivity."
FT /evidence="ECO:0000269|PubMed:2002066"
FT MUTAGEN 77
FT /note="E->P: 50-fold reduction in bioactivity."
FT /evidence="ECO:0000269|PubMed:2002066"
FT MUTAGEN 80
FT /note="L->P: 450-fold reduction in bioactivity."
FT /evidence="ECO:0000269|PubMed:2002066"
FT MUTAGEN 124
FT /note="L->P: 5500-fold reduction in bioactivity."
FT /evidence="ECO:0000269|PubMed:2002066"
FT CONFLICT 25
FT /note="T -> I (in Ref. 5; CAA29336 and 7; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 114
FT /note="V -> A (in Ref. 6; AAA37483)"
FT /evidence="ECO:0000305"
FT CONFLICT 139
FT /note="G -> S (in Ref. 5; CAA29336)"
FT /evidence="ECO:0000305"
FT CONFLICT 139
FT /note="G -> V (in Ref. 2; CAA26820, 3; no nucleotide entry,
FT 4; CAA26192/CAA26193 and 6; AAA37483)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 141 AA; 16091 MW; 209C7CBB4FF77349 CRC64;
MWLQNLLFLG IVVYSLSAPT RSPITVTRPW KHVEAIKEAL NLLDDMPVTL NEEVEVVSNE
FSFKKLTCVQ TRLKIFEQGL RGNFTKLKGA LNMTASYYQT YCPPTPETDC ETQVTTYADF
IDSLKTFLTD IPFECKKPGQ K
