CSF2_RAT
ID CSF2_RAT Reviewed; 144 AA.
AC P48750;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Granulocyte-macrophage colony-stimulating factor;
DE Short=GM-CSF;
DE AltName: Full=Colony-stimulating factor;
DE Short=CSF;
DE Flags: Precursor;
GN Name=Csf2; Synonyms=Csfgm;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 18-144.
RC STRAIN=Lewis; TISSUE=Splenocyte;
RX PubMed=8225444; DOI=10.1007/bf00171806;
RA Smith L.R., Lundeen K.A., Diveley J.P., Carlo D.J., Brostoff S.W.;
RT "Nucleotide sequence of the Lewis rat granulocyte-macrophage colony
RT stimulating factor.";
RL Immunogenetics 39:80-80(1994).
CC -!- FUNCTION: Cytokine that stimulates the growth and differentiation of
CC hematopoietic precursor cells from various lineages, including
CC granulocytes, macrophages, eosinophils and erythrocytes. {ECO:0000250}.
CC -!- SUBUNIT: Monomer. The signaling GM-CSF receptor complex is a dodecamer
CC of two head-to-head hexamers of two alpha, two beta, and two ligand
CC subunits (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the GM-CSF family. {ECO:0000305}.
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DR EMBL; AABR03075230; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00620; AAA18281.1; -; mRNA.
DR PIR; I46269; I46269.
DR RefSeq; NP_446304.1; NM_053852.1.
DR AlphaFoldDB; P48750; -.
DR SMR; P48750; -.
DR STRING; 10116.ENSRNOP00000034235; -.
DR GlyGen; P48750; 3 sites.
DR PhosphoSitePlus; P48750; -.
DR PaxDb; P48750; -.
DR PRIDE; P48750; -.
DR Ensembl; ENSRNOT00000032333; ENSRNOP00000034235; ENSRNOG00000026805.
DR GeneID; 116630; -.
DR KEGG; rno:116630; -.
DR UCSC; RGD:621065; rat.
DR CTD; 1437; -.
DR RGD; 621065; Csf2.
DR eggNOG; ENOG502TDUI; Eukaryota.
DR GeneTree; ENSGT00390000013425; -.
DR HOGENOM; CLU_152286_0_0_1; -.
DR InParanoid; P48750; -.
DR OMA; VTRPWKH; -.
DR OrthoDB; 1412355at2759; -.
DR PhylomeDB; P48750; -.
DR TreeFam; TF338611; -.
DR Reactome; R-RNO-512988; Interleukin-3, Interleukin-5 and GM-CSF signaling.
DR Reactome; R-RNO-5673001; RAF/MAP kinase cascade.
DR Reactome; R-RNO-912526; Interleukin receptor SHC signaling.
DR PRO; PR:P48750; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000026805; Expressed in lung and 7 other tissues.
DR Genevisible; P48750; RN.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0030526; C:granulocyte macrophage colony-stimulating factor receptor complex; ISO:RGD.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0005125; F:cytokine activity; IDA:RGD.
DR GO; GO:0005129; F:granulocyte macrophage colony-stimulating factor receptor binding; IEA:InterPro.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0097011; P:cellular response to granulocyte macrophage colony-stimulating factor stimulus; ISO:RGD.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0097028; P:dendritic cell differentiation; IDA:RGD.
DR GO; GO:0001892; P:embryonic placenta development; ISO:RGD.
DR GO; GO:0050673; P:epithelial cell proliferation; TAS:RGD.
DR GO; GO:0042045; P:epithelial fluid transport; IDA:RGD.
DR GO; GO:0038157; P:granulocyte-macrophage colony-stimulating factor signaling pathway; ISO:RGD.
DR GO; GO:0001821; P:histamine secretion; IDA:RGD.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0042116; P:macrophage activation; IMP:RGD.
DR GO; GO:0030225; P:macrophage differentiation; ISO:RGD.
DR GO; GO:0030224; P:monocyte differentiation; ISO:RGD.
DR GO; GO:0030099; P:myeloid cell differentiation; IBA:GO_Central.
DR GO; GO:0043011; P:myeloid dendritic cell differentiation; ISO:RGD.
DR GO; GO:2001240; P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0030223; P:neutrophil differentiation; ISO:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:0032747; P:positive regulation of interleukin-23 production; ISO:RGD.
DR GO; GO:0070665; P:positive regulation of leukocyte proliferation; ISO:RGD.
DR GO; GO:0010744; P:positive regulation of macrophage derived foam cell differentiation; ISO:RGD.
DR GO; GO:0045651; P:positive regulation of macrophage differentiation; TAS:RGD.
DR GO; GO:0071803; P:positive regulation of podosome assembly; ISO:RGD.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:RGD.
DR GO; GO:0006468; P:protein phosphorylation; ISO:RGD.
DR GO; GO:0007259; P:receptor signaling pathway via JAK-STAT; ISO:RGD.
DR GO; GO:0042127; P:regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0045187; P:regulation of circadian sleep/wake cycle, sleep; IMP:RGD.
DR GO; GO:0010468; P:regulation of gene expression; ISO:RGD.
DR GO; GO:0034405; P:response to fluid shear stress; IEP:RGD.
DR GO; GO:0034021; P:response to silicon dioxide; IEP:RGD.
DR CDD; cd00040; CSF2; 1.
DR Gene3D; 1.20.1250.10; -; 1.
DR InterPro; IPR009079; 4_helix_cytokine-like_core.
DR InterPro; IPR000773; GM_colony-stim-fac.
DR PANTHER; PTHR10059; PTHR10059; 1.
DR Pfam; PF01109; GM_CSF; 1.
DR PRINTS; PR00693; GMCSFACTOR.
DR SMART; SM00040; CSF2; 1.
DR SUPFAM; SSF47266; SSF47266; 1.
DR PROSITE; PS00702; GM_CSF; 1.
PE 2: Evidence at transcript level;
KW Cytokine; Disulfide bond; Glycoprotein; Growth factor; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000250"
FT CHAIN 18..144
FT /note="Granulocyte-macrophage colony-stimulating factor"
FT /id="PRO_0000144833"
FT CARBOHYD 22
FT /note="O-linked (GalNAc...) serine"
FT /evidence="ECO:0000250"
FT CARBOHYD 27
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000250"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 71..113
FT /evidence="ECO:0000250"
FT DISULFID 105..138
FT /evidence="ECO:0000250"
SQ SEQUENCE 144 AA; 16562 MW; 7385E93CC40836B1 CRC64;
MWLQNLLFLG IVVYSFSAPT RSPNPVTRPW KHVDAIKEAL SLLNDMRALE NEKNEDVDII
SNEFSIQRPT CVQTRLKLYK QGLRGNLTKL NGALTMIASH YQTNCPPTPE TDCEIEVTTF
EDFIKNLKGF LFDIPFDCWK PVQK
