ID   CSG_METFV               Reviewed;         593 AA.
AC   P27373; E3GXJ6;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Cell surface glycoprotein;
DE   AltName: Full=S-layer protein;
DE   Flags: Precursor;
GN   Name=slgA; OrderedLocusNames=Mfer_0225;
OS   Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanothermaceae; Methanothermus.
OX   NCBI_TaxID=523846;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 23-42.
RC   STRAIN=ATCC 43054 / DSM 2088 / JCM 10308 / V24 S;
RX   PubMed=1712296; DOI=10.1111/j.1432-1033.1991.tb16102.x;
RA   Broeckl G., Behr M., Fabry S., Hensel R., Kaudewitz H., Biendl E.,
RA   Koenig H.;
RT   "Analysis and nucleotide sequence of the genes encoding the surface-layer
RT   glycoproteins of the hyperthermophilic methanogens Methanothermus fervidus
RT   and Methanothermus sociabilis.";
RL   Eur. J. Biochem. 199:147-152(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43054 / DSM 2088 / JCM 10308 / V24 S;
RX   PubMed=21304736; DOI=10.4056/sigs.1283367;
RA   Anderson I., Djao O.D., Misra M., Chertkov O., Nolan M., Lucas S.,
RA   Lapidus A., Del Rio T.G., Tice H., Cheng J.F., Tapia R., Han C.,
RA   Goodwin L., Pitluck S., Liolios K., Ivanova N., Mavromatis K.,
RA   Mikhailova N., Pati A., Brambilla E., Chen A., Palaniappan K., Land M.,
RA   Hauser L., Chang Y.J., Jeffries C.D., Sikorski J., Spring S., Rohde M.,
RA   Eichinger K., Huber H., Wirth R., Goker M., Detter J.C., Woyke T.,
RA   Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P.,
RA   Kyrpides N.C.;
RT   "Complete genome sequence of Methanothermus fervidus type strain (V24S).";
RL   Stand. Genomic Sci. 3:315-324(2010).
RN   [3]
RP   GLYCOSYLATION.
RC   STRAIN=ATCC 43054 / DSM 2088 / JCM 10308 / V24 S;
RX   PubMed=8262914; DOI=10.1016/s0021-9258(19)74185-4;
RA   Karcher U., Schroder H., Haslinger E., Allmaier G., Schreiner R.,
RA   Wieland F., Haselbeck A., Konig H.;
RT   "Primary structure of the heterosaccharide of the surface glycoprotein of
RT   Methanothermus fervidus.";
RL   J. Biol. Chem. 268:26821-26826(1993).
CC   -!- FUNCTION: The S-layer is a paracrystalline mono-layered assembly of
CC       proteins which coat the surface of the cell.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer. Note=This archaea
CC       is covered by an S-layer with hexagonal symmetry.
CC   -!- PTM: N-glycosylated; contains glycans composed of methyl-Man, Man and
CC       GalNAc residues in a molar ratio of 2:3:1.
CC       {ECO:0000269|PubMed:8262914}.
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DR   EMBL; X58297; CAA41230.1; -; Genomic_DNA.
DR   EMBL; CP002278; ADP77028.1; -; Genomic_DNA.
DR   PIR; S16225; S16225.
DR   RefSeq; WP_013413306.1; NC_014658.1.
DR   AlphaFoldDB; P27373; -.
DR   SMR; P27373; -.
DR   iPTMnet; P27373; -.
DR   EnsemblBacteria; ADP77028; ADP77028; Mfer_0225.
DR   GeneID; 9961940; -.
DR   KEGG; mfv:Mfer_0225; -.
DR   HOGENOM; CLU_459779_0_0_2; -.
DR   OrthoDB; 82089at2157; -.
DR   Proteomes; UP000002315; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR006633; Carb-bd_sugar_hydrolysis-dom.
DR   InterPro; IPR007742; NosD_dom.
DR   InterPro; IPR022441; Para_beta_helix_rpt-2.
DR   InterPro; IPR006626; PbH1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF05048; NosD; 2.
DR   SMART; SM00722; CASH; 2.
DR   SMART; SM00710; PbH1; 8.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   TIGRFAMs; TIGR03804; para_beta_helix; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Cell wall biogenesis/degradation; Direct protein sequencing;
KW   Glycoprotein; Reference proteome; S-layer; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:1712296"
FT   CHAIN           23..593
FT                   /note="Cell surface glycoprotein"
FT                   /id="PRO_0000032618"
FT   CARBOHYD        29
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:8262914"
FT   CARBOHYD        58
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        66
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        74
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        114
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        122
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        145
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        148
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        158
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        176
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        208
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        231
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        326
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        336
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        340
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        431
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        471
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        500
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        516
FT                   /note="N-linked (GalNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   593 AA;  65481 MW;  E064669967B7611C CRC64;
     MRKFTLLMLL LIVISMSGIA GAAEVKNLNT SKTFTKIQEA IDDPSTTDGN IIIVGPGNYT
     ENILVNKSLT LKSNGSAIIN AVSSEKSTIT IKANNVWIEG FIIIGGKNGI YMENVTGCTI
     TNNTIQNAFV SGWEYYGGNG ICLVNSTNNT ITNNIIRNNT WNGINVCESK GNIIKNNTIM
     YSGGIGIYVW GFNKFEGNNI IENNRIINAT YGGIYLFRPS NNKICRNYIA NVSSGGGGMS
     GAICIDVSDY NIVKDNIGIN CDGGLFTDGM IGNEITNNIF KNCKVAVSES TYGPASRNNK
     IYGNYFINYE TAISDPKGEL VDNIWNTTEG GNYWSNYTGN NTGDGTGNIP YYYDNKPLVV
     DLAIEDIAAK PSGIEVRVKN LGKADIKKID PLTKLKIKIS CDNDVYETFI DPLSAGESQI
     VRWDKIVPEG NHTIKAEIPY SAEGYLIGTN IRDADISNNV FSKIVQGFVQ NKTFTITLTN
     LGKSTITIKY YISIYTNPVN GTKVSYRELT ITLKPNETKT IELGKYPFKY AVSGTMIVKN
     PSRYRIPLNL RIKYEIEGLN PQMREISKYI APRGEFRYIA RYTGKEEGYA DVW